Identification
Name:Formate hydrogenlyase subunit 3
Synonyms:
  • FHL subunit 3
  • Hydrogenase-3 component C
Gene Name:hycC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in NADH dehydrogenase (ubiquinone) activity
Specific Function:Specific function unknown
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+2.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
catalytic activity
NADH dehydrogenase (quinone) activity
NADH dehydrogenase (ubiquinone) activity
NADH dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain
Gene Properties
Blattner:b2723
Gene OrientationCounterclockwise
Centisome Percentage:61.33
Left Sequence End2845437
Right Sequence End2847263
Gene Sequence:
>1827 bp
ATGCATTATATGAAGTGGATTTATCCACGCCGCTTACGCAATCAAATGATCCTGATGGCA
ATCCTGATGGTCATTGTCCCAACGCTTACTATTGGTTATATCGTAGAAACGGAAGGACGT
TCAGCAGTCTTATCTGAAAAAGAGAAAAAACTTTCTGCCGTGGTCAACCTGCTTAATCAG
GCACTAGGCGATCGCTATGATCTCTACATCGACTTACCACGTGAGGAGCGTATCCGCGCA
TTAAATGCAGAACTTGCCCCCATTACCGAAAATATCACTCACGCCTTCCCTGGCATCGGT
GCTGGTTATTACAACAAAATGCTGGATGCGATAATCACCTACGCGCCTTCAGCGCTATAT
CAGAATAATGTCGGCGTTACCATTGCCGCAGATCACCCTGGTCGCGAAGTGATGCGTACA
AATACCCCTTTAGTTTATTCAGGCAGGCAGGTGCGCGGCGATATTTTGAATTCAATGCTC
CCCATTGAGCGTAATGGTGAAATCCTCGGCTATATCTGGGCCAATGAATTAACCGAAGAT
ATTCGCCGCCAGGCCTGGAAAATGGATGTGAGGATTATCATTGTGCTCACCGCTGGTTTG
CTGATAAGCCTGCTGTTGATTGTCCTTTTCTCCCGTCGCCTGAGCGCCAATATTGATATC
ATCACCGATGGCCTCTCGACTCTGGCACAAAATATTCCCACTCGATTACCACAATTGCCC
GGTGAAATGGGGCAAATCAGTCAGAGTGTTAATAACCTCGCCCAGGCACTGCGTGAAACG
CGGACACTTAACGATCTGATTATTGAAAACGCTGCCGATGGCGTCATTGCCATTGACCGC
CAGGGTGATGTAACCACCATGAACCCAGCAGCAGAAGTTATCACTGGCTATCAACGCCAT
GAACTGGTAGGGCAGCCTTACTCCATGTTGTTCGACAATACTCAGTTCTACAGTCCAGTA
CTGGATACGCTGGAACATGGCACCGAACATGTGGCGCTGGAGATCAGTTTTCCAGGTCGT
GACCGCACCATTGAACTCAGTGTCACTACCAGTCGTATTCATAACACGCACGGTGAAATG
ATAGGTGCTTTGGTGATTTTCTCTGATTTAACTGCCCGCAAAGAAACCCAGCGCCGCATG
GCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTA
CGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGAC
CCAATACATCAGGAATATCTGTCCGTAGTACTCAAAGAAATCGATTCAATTAACAAAGTT
ATTCAGCAATTGCTCGAATTTTCACGTCCACGCCACAGTCAATGGCAACAAGTCAGCCTC
AATGCATTGGTTGAAGAAACTCTGGTACTGGTACAAACCGCCGGCGTACAAGCGCGGGTC
GACTTCATAAGCGAACTGGATAATGAATTAAGCCCGATTAACGCCGATCGTGAACTGCTC
AAACAGGTACTACTGAATATCCTGATCAATGCCGTCCAGGCTATCAGCGCACGAGGGAAA
ATTCGCATTCAAACCTGGCAATACAGCGACTCACAACAGGCCATTTCGATAGAGGACAAC
GGCTGTGGCATTGATCTCTCGCTGCAAAAAAAGATCTTCGATCCCTTTTTCACCACCAAA
GCCTCAGGAACCGGGCTTGGTCTGGCGTTAAGTCAACGCATCATTAATGCCCATCAGGGT
GATATTCGCGTCGCCAGTTTGCCGGGCTACGGCGCAACCTTCACGCTTATTTTACCGATC
AACCCGCAGGGAAATCAGACTGTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:608
Protein Molecular Weight:64076
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 10-26
  • 44-67
  • 76-93
  • 116-140
  • 153-173
  • 197-218
  • 229-251
  • 258-280
  • 296-312
  • 416-440
  • 453-476
  • 502-521
Protein Sequence:
>Formate hydrogenlyase subunit 3
MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVGV
SGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAAAVC
AVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRLGTLLLAIACWLLWQRYGTLD
LRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALFSTVVMKI
GLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALVEHNIQRLLAYHTLENIGIIL
LGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGG
IGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGL
AITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLL
PMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYD
HEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLV
VIIVSRGA
References
External Links:
ResourceLink
Uniprot ID:P16429
Uniprot Name:HYCC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736874
Ecogene ID:EG10476
Ecocyc:EG10476
ColiBase:b2723
Kegg Gene:b2723
EchoBASE ID:EB0471
CCDB:HYCC_ECOLI
BacMap:16130630
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bohm, R., Sauter, M., Bock, A. (1990). "Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Mol Microbiol 4:231-243. Pubmed: 2187144
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553