ECMDB: Aromatic amino acid transport protein AroP (P15993)

Identification

Name:

Aromatic amino acid transport protein AroP

Synonyms:

General aromatic amino acid permease

Gene Name:

aroP

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in transport

Specific Function:

Permease that is involved in the transport across the cytoplasmic membrane of the aromatic amino acids (phenylalanine, tyrosine, and tryptophan)

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Phenylalanine metabolism PW000921
inner membrane transport PW000786
tRNA charging PW000799

KEGG Pathways:

Phenylalanine metabolism ec00360

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590

Metabolites:

ECMDB ID	Name	View
ECMDB20144	D-Phenylalanine	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00177	L-Histidine	MetaboCard
ECMDB00159	L-Phenylalanine	MetaboCard
ECMDB00929	L-Tryptophan	MetaboCard
ECMDB00158	L-Tyrosine	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
active transmembrane transporter activity
amine transmembrane transporter activity
amino acid transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
establishment of localization
transmembrane transport
transport

Gene Properties

Blattner:

b0112

Gene Orientation

Counterclockwise

Centisome Percentage:

2.59

Left Sequence End

120178

Right Sequence End

121551

Gene Sequence:

>1374 bp
ATGATGGAAGGTCAACAGCACGGCGAGCAGCTAAAGCGCGGCCTTAAAAACCGCCATATT
CAGCTTATCGCGCTGGGTGGCGCGATAGGGACCGGGTTATTCCTGGGTAGCGCCTCCGTA
ATACAGTCCGCAGGGCCAGGGATTATCCTGGGTTACGCCATTGCTGGTTTTATCGCCTTT
CTGATCATGCGTCAGCTGGGTGAAATGGTGGTCGAAGAACCTGTCGCAGGCTCCTTTAGC
CACTTTGCTTATAAATACTGGGGCAGTTTTGCCGGTTTCGCCTCTGGCTGGAACTACTGG
GTACTGTACGTTTTAGTTGCCATGGCTGAGCTGACTGCCGTGGGTAAATACATTCAGTTC
TGGTATCCGGAAATCCCCACCTGGGTTTCTGCCGCCGTATTCTTTGTGGTGATTAACGCC
ATCAACCTGACCAACGTTAAAGTGTTTGGCGAGATGGAGTTCTGGTTTGCCATTATCAAA
GTTATCGCGGTGGTAGCGATGATCATCTTCGGCGGCTGGCTGCTATTCAGTGGCAACGGC
GGCCCGCAGGCGACCGTTAGCAACCTGTGGGATCAGGGTGGTTTCCTGCCGCACGGCTTC
ACCGGGCTGGTGATGATGATGGCGATTATCATGTTCTCGTTCGGTGGTCTGGAACTGGTG
GGGATCACCGCAGCAGAAGCTGATAACCCGGAGCAAAGTATACCGAAAGCAACTAACCAG
GTTATCTACCGCATCCTGATTTTCTATATTGGTTCGTTAGCCGTTCTGCTCTCACTGATG
CCGTGGACCCGCGTTACCGCCGATACCAGTCCGTTTGTGCTGATCTTCCACGAGTTAGGC
GATACCTTTGTGGCGAATGCGCTGAACATCGTGGTACTGACTGCGGCGCTCTCCGTGTAC
AACAGCTGCGTATATTGCAACAGCCGTATGCTGTTTGGTCTGGCACAACAGGGTAATGCG
CCAAAAGCGCTGGCGTCTGTCGATAAACGTGGTGTACCAGTAAATACCATTCTGGTGTCT
GCACTGGTAACGGCGTTGTGCGTACTGATTAACTACCTTGCCCCAGAGTCCGCTTTCGGA
CTGTTAATGGCGCTGGTGGTATCTGCACTGGTAATCAACTGGGCGATGATTAGCCTGGCG
CATATGAAATTCCGTCGCGCCAAGCAGGAACAAGGCGTGGTAACTCGCTTCCCTGCTCTG
CTTTATCCGCTGGGTAACTGGATCTGCCTGCTGTTTATGGCGGCGGTACTGGTGATTATG
CTGATGACCCCAGGAATGGCGATTTCGGTATACCTGATCCCGGTATGGCTGATCGTGTTA
GGTATCGGCTATCTGTTTAAAGAGAAAACCGCCAAAGCCGTAAAAGCGCATTAA

Protein Properties

Pfam Domain Function:

AA_permease (PF00324 )

Protein Residues:

457

Protein Molecular Weight:

49690

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

21-41
43-63
87-107
118-138
156-176
202-222
241-261
272-292
334-354
359-379
401-421
426-446

Protein Sequence:

>Aromatic amino acid transport protein AroP
MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAGPGIILGYAIAGFIAF
LIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLYVLVAMAELTAVGKYIQF
WYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKVIAVVAMIIFGGWLLFSGNG
GPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLELVGITAAEADNPEQSIPKATNQ
VIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFHELGDTFVANALNIVVLTAALSVY
NSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVNTILVSALVTALCVLINYLAPESAFG
LLMALVVSALVINWAMISLAHMKFRRAKQEQGVVTRFPALLYPLGNWICLLFMAAVLVIM
LMTPGMAISVYLIPVWLIVLGIGYLFKEKTAKAVKAH

References

External Links:

Resource	Link
Uniprot ID:	P15993
Uniprot Name:	AROP_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674334
Ecogene ID:	EG10084
Ecocyc:	EG10084
ColiBase:	b0112
Kegg Gene:	b0112
EchoBASE ID:	EB0082
CCDB:	AROP_ECOLI
BacMap:	16128105

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Cosgriff, A. J., Pittard, A. J. (1997). "A topological model for the general aromatic amino acid permease, AroP, of Escherichia coli." J Bacteriol 179:3317-3323. Pubmed: 9150230
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Honore, N., Cole, S. T. (1990). "Nucleotide sequence of the aroP gene encoding the general aromatic amino acid transport protein of Escherichia coli K-12: homology with yeast transport proteins." Nucleic Acids Res 18:653. Pubmed: 2408019