Shikimate dehydrogenase (P15770)

Identification
Name:Shikimate dehydrogenase
Synonyms:Not Available
Gene Name:aroE
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Dehydro-shikimate + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0NADP + 1.0Shikimic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Shikimic acid + 1.0NADP ↔ 1.03-Dehydro-shikimate + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Dehydro-shikimate + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0NADP + 1.0Shikimic acid
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADP + 1.0Shikimic acid ← 1.0Hydrogen ion + 1.0NADPH + 1.03-Dehydro-shikimate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB040483-Dehydro-shikimateMetaboCard
ECMDB02260Gallic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB03070Shikimic acidMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
NADP or NADPH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
shikimate 5-dehydrogenase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b3281
Gene OrientationCounterclockwise
Centisome Percentage:73.89
Left Sequence End3428042
Right Sequence End3428860
Gene Sequence:
>819 bp
ATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCCGACCATCATTTA
GGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT
GCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG
TATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGT
GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCC
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG
CAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTC
GGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATC
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTC
GAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTA
GGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGATGAACGATCGCGAAATGTTA
GTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC
CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCAC
TGGCTGGACTATCCACATCTACCTTATTCCCCCGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:272
Protein Molecular Weight:29413
Protein Theoretical pI:5
PDB File:1NYT
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Shikimate dehydrogenase
METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFINTLNAFFSAGGKGANV
TVPFKEEAFARADELTERAALAGAVNTLMRLEDGRLLGDNTDGVGLLSDLERLSFIRPGL
RILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAKLFAHTGSIQALSMDELEGHE
FDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKGKTPFLAWCEQRGSKRNADGLGM
LVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA
References
External Links:
ResourceLink
Uniprot ID:P15770
Uniprot Name:AROE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674586
PDB ID:1NYT
Ecogene ID:EG10077
Ecocyc:EG10077
ColiBase:b3281
Kegg Gene:b3281
EchoBASE ID:EB0075
CCDB:AROE_ECOLI
BacMap:16131162
General Reference:
  • Anton, I. A., Coggins, J. R. (1988). "Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase." Biochem J 249:319-326. Pubmed: 3277621
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Maclean, J., Campbell, S. A., Pollock, K., Chackrewarthy, S., Coggins, J. R., Lapthorn, A. J. (2000). "Crystallization and preliminary X-ray analysis of shikimate dehydrogenase from Escherichia coli." Acta Crystallogr D Biol Crystallogr 56:512-515. Pubmed: 10739937
  • Michel, G., Roszak, A. W., Sauve, V., Maclean, J., Matte, A., Coggins, J. R., Cygler, M., Lapthorn, A. J. (2003). "Structures of shikimate dehydrogenase AroE and its Paralog YdiB. A common structural framework for different activities." J Biol Chem 278:19463-19472. Pubmed: 12637497