| Identification |
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| Name: | Protein traI |
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| Synonyms: | |
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| Gene Name: | traI |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Replication, recombination and repair |
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| Specific Function: | TraI has been identified as DNA helicase I and it also has an additional activity of site-specific nicking at oriT. DNA helicase I is a potent DNA-dependent ATPase |
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| Cellular Location: | Cytoplasmic |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Complex Reactions: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| adenyl nucleotide binding | | adenyl ribonucleotide binding | | ATP binding | | binding | | catalytic activity | | DNA binding | | DNA helicase activity | | helicase activity | | hydrolase activity | | hydrolase activity, acting on acid anhydrides | | hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides | | nucleic acid binding | | nucleoside binding | | nucleoside-triphosphatase activity | | purine nucleoside binding | | pyrophosphatase activity | | Process |
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| conjugation | | multi-organism process |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >5271 bp
ATGATGAGTATTGCGCAGGTCAGATCGGCCGGAAGTGCCGGGAACTATTATACCGACAAG
GATAATTACTATGTGCTGGGCAGCATGGGAGAACGCTGGGCCGGCAGGGGGGCTGAACAG
CTGGGGCTGCAGGGCAGTGTCGATAAGGATGTTTTTACCCGTCTTCTGGAGGGCAGGCTG
CCGGACGGAGCGGATCTAAGCCGCATGCAGGATGGCAGTAACAGGCATCGTCCCGGCTAC
GATCTGACCTTCTCCGCCCCCAAAAGTGTCTCCATGATGGCCATGTTAGGTGGCGATAAG
CGCCTGATTGATGCACATAATCAGGCCGTGGATTTTGCTGTCCGTCAGGTGGAGGCGCTG
GCCTCCACACGGGTGATGACGGACGGACAGTCAGAAACGGTGCTGACCGGTAATCTGGTG
ATGGCACTGTTTAACCACGACACCAGTCGCGATCAGGAACCACAGTTACACACGCATGCG
GTGGTGGCTAATGTCACGCAGCATAATGGCGAATGGAAGACACTGAGCAGTGACAAAGTG
GGGAAAACGGGGTTCATTGAGAATGTGTACGCTAATCAGATTGCCTTTGGCAGGCTCTAC
CGGGAAAAACTGAAAGAGCAGGTTGAGGCGCTGGGCTATGAAACTGAAGTGGTTGGTAAG
CACGGTATGTGGGAAATGCCGGGTGTACCGGTGGAGGCCTTTTCCGGACGCTCACAGACT
ATCCGGGAGGCCGTCGGGGAAGACGCCTCGCTGAAATCCCGGGATGTGGCGGCGCTGGAT
ACGCGTAAATCCAAACAGCATGTCGATCCGGAGATCAAAATGGCCGAGTGGATGCAGACG
CTGAAGGAAACCGGGTTCGACATCCGGGCATATCGTGACGCGGCGGATCAGCGTGCGGAT
CTCCGCACGCTGACGCCCGGGCCTGCTTCACAGGACGGGCCGGATGTGCAGCAGGCGGTG
ACACAGGCGATTGCCGGATTAAGTGAACGAAAAGTGCAGTTCACGTACACGGACGTACTG
GCCAGAACGGTCGGCATACTGCCGCCGGAAAATGGTGTGATTGAACGGGCACGCGCCGGT
ATCGATGAGGCCATCAGCCGTGAGCAGCTTATCCCCCTCGACCGTGAGAAGGGGCTGTTC
ACGTCCGGGATTCATGTGCTCGATGAGCTGTCAGTCCGGGCACTCAGTCGTGACATCATG
AAACAGAACCGGGTGACCGTACATCCGGAGAAAAGTGTTCCCCGGACGGCCGGTTACAGC
GATGCCGTCAGCGTGCTGGCACAGGATCGCCCGTCGCTGGCCATTGTGTCCGGGCAGGGC
GGTGCAGCCGGGCAGCGTGAGCGGGTGGCTGAACTGGTCATGATGGCCCGGGAGCAGGGG
CGGGAGGTGCAGATTATCGCTGCTGACCGTCGCTCGCAGATGAACATGAAGCAGGATGAA
CGGTTGTCCGGTGAGCTGATAACCGGACGTCGTCAGCTGCTGGAAGGCATGGCCTTCACG
CCGGGCAGTACTGTTATCGTTGACCAGGGCGAAAAACTCTCCCTGAAAGAGACGTTAACC
CTGCTGGACGGTGCCGCACGTCATAACGTACAGGTCCTGATAACCGACAGCGGGCAGCGA
ACCGGTACAGGCAGTGCACTGATGGCCATGAAGGATGCCGGGGTGAACACATATCGCTGG
CAGGGGGGAGAACAGCGACCGGCCACCATCATCAGTGAACCGGACCGTAATGTCCGCTAT
GCCCGGCTGGCAGGAGATTTTGCGGCCAGCGTGAAAGCCGGAGAAGAGAGCGTGGCACAG
GTCAGCGGGGTACGGGAACAGGCCATACTGACACAGGCCATTCGCAGTGAGCTGAAAACA
CAGGGCGTGCTCGGACTCCCGGAGGTGACCATGACTGCCCTTTCACCGGTCTGGCTGGAC
AGCCGGAGCCGTTATCTGCGGGATATGTACCGTCCGGGGATGGTGATGGAGCAGTGGAAC
CCGGAGACACGCAGTCATGACCGCTATGTTATCGACCGGGTGACGGCGCAGAGTCACAGC
CTGACCCTGCGGGATGCGCAGGGTGAAACGCAGGTGGTGCGTATTTCCTCCCTGGACAGC
AGCTGGTCGCTGTTCCGGCCGGAAAAAATGCCGGTGGCAGACGGCGAGCGACTGAGGGTG
ACAGGGAAAATTCCCGGACTCCGCGTCTCCGGCGGTGACCGCCTGCAGGTGGCATCCGTC
AGTGAAGATGCGATGACGGTTGTTGTGCCGGGACGGGCTGAACCGGCCACCCTGCCTGTG
AGCGATTCACCGTTCACGGCACTGAAGCTGGAGAACGGCTGGGTGGAAACGCCCGGGCAT
TCCGTCAGCGACAGTGCGACGGTTTTTGCCTCCGTCACACAGATGGCAATGGACAATGCC
ACCCTGAACGGTCTGGCCCGCAGTGGTCGTGATGTCCGGCTGTATTCCTCACTGGATGAA
ACCCGTACTGCGGAAAAACTTGCCCGCCATCCGTCCTTTACGGTGGTTTCTGAGCAGATA
AAGACGCGGGCCGGTGAGACATCGCTGGAAACGGCTATCAGTCATCAGAAGTCCGCACTT
CACACGCCGGCACAGCAGGCCATTCATCTTGCCCTTCCGGTGGTGGAAAGTAAAAAACTG
GCCTTCAGCATGGTGGACCTGCTGACAGAGGCAAAGTCGTTTGCTGCAGAAGGAACCGGT
TTTACTGAACTGGGAGGGGAAATCAATGCGCAGATAAAACGGGGTGATTTACTGTATGTG
GATGTGGCAAAAGGCTATGGCACAGGTCTGCTGGTTTCCCGTGCGTCGTATGAGGCAGAA
AAGAGCATTCTTCGCCATATTCTCGAAGGTAAGGAGGCGGTCATGCCGCTGATGGAGAGA
GTACCTGGCGAACTCATGGAGAAACTGACATCAGGACAGCGTGCCGCCACCCGCATGATA
CTGGAAACGTCCGACCGTTTCACGGTGGTACAGGGCTATGCCGGTGTGGGTAAGACCACA
CAGTTCCGGGCGGTGATGTCAGCCGTGAACATGCTGCCGGAGAGTGAGCGTCCCCGAGTC
GTGGGGCTGGGTCCCACACACCGTGCGGTCGGGGAGATGCGCAGCGCCGGCGTGGATGCG
CAGACACTGGCGTCCTTTCTGCATGACACGCAGCTGCAGCAGCGCAGCGGAGAAACGCCG
GATTTCAGCAACACGCTGTTCCTGCTCGATGAGAGCTCAATGGTGGGCAATACCGACATG
GCACGGGCATACGCCCTGATTGCGGCCGGTGGCGGTAGGGCGGTGGCCAGTGGTGACACG
GACCAGCTGCAGGCCATCGCGCCCGGTCAGCCTTTCCGTCTCCAGCAGACGCGCAGTGCT
GCCGATGTGGCCATCATGAAGGAGATTGTGCGTCAGACGCCGGAACTGCGGGAGGCGGTA
TACAGCCTGATTAACCGGGATGTGGAAAGGGCACTGTCCGGGCTTGAGAGTGTGAAACCG
TCTCAGGTGCCACGTCAGGAGGGCGCATGGGCACCGGAGCACTCCGTGACGGAGTTCAGT
CACAGCCAGGAAGCGAAACTGGCAGAAGCGCAGCAGAAGGCGATGCTGAAAGGCGAGGCT
TTTCCGGATGTCCCCATGACACTGTATGAAGCCATTGTCCGCGACTATACCGGCAGAACA
CCGGAAGCACGGGAGCAGACGCTGATTGTCACGCACCTGAATGAGGACCGGCGCGTACTG
AACAGCATGATTCATGATGTACGGGAAAAGGCCGGTGAGCTGGGGAAAGAGCAGGTCATG
GTGCCTGTCCTGAACACAGCGAATATACGTGACGGGGAGCTGCGTCGTCTCTCCACCTGG
GAGACACATCGGGACGCACTTGTCCTGGTGGATAATGTGTATCACCGGATTGCCGGTATC
AGTAAGGATGACGGGCTGATAACCCTGCAGGATGCGGAAGGTAACACGCGGTTGATTTCT
CCCCGGGAGGCGGTGGCTGAAGGTGTCACACTGTACACCCCGGACACCATCAGGGTGGGG
ACCGGTGACCGGATGCGCTTCACGAAGAGTGACCGGGAGCGCGGTTATGTGGCCAACAGC
GTCTGGACGGTGACAGCAGTTTCCGGTGACAGTGTCACGCTGTCGGACGGACAGCAGACC
CGGGAGATTCGCCCCGGCCAGGAGCAGGCAGAGCAACATATTGACCTGGCCTATGCCATC
ACCGCTCACGGTGCGCAGGGGGCAAGTGAAACCTTTGCCATTGCGCTTGAAGGAACGGAA
GGTAACCGGAAACTGATGGCCGGCTTTGAGTCAGCCTACGTGGCCCTGTCGCGTATGAAG
CAGCATGTGCAGGTGTACACCGATAACCGTCAGGGCTGGACGGATGCCATTAACAATGCC
GTACAGAAAGGAACGGCCCACGATGTATTTGAGCCGAAACCGGACCGGGAGGTCATGAAT
GCAGAGCGGCTGTTCAGTACGGCGCGGGAACTGCGGGACGTGGCGGCAGGGCGTGCTGTT
CTCCGTCAGGCGGGGCTGGCCGGGGGAGACAGTCCGGCACGGTTTATTGCACCGGGGCGC
AAATATCCGCAGCCGTATGTGGCACTGCCGGCGTTTGACCGTAACGGCAAGTCAGCAGGT
ATCTGGCTGAACCCACTGACCACGGATGACGGAAACGGGCTGCGGGGATTCAGTGGTGAA
GGACGGGTGAAAGGCAGCGGGGATGCGCAGTTCGTGGCCCTGCAGGGCAGCCGTAACGGA
GAGAGCCTGCTGGCTGATAATATGCAGGATGGTGTCCGGATTGCCCGTGATAATCCTGAC
AGCGGTGTGGTGGTGAGAATCGCCGGTGAAGGTCGTCCGTGGAATCCCGGTGCCATCACC
GGTGGTCGCGTGTGGGGGGATATCCCGGACAACAGTGTCCAGCCGGGAGCCGGAAATGGC
GAACCGGTCACGGCAGAGGTGCTGGCACAGCGGCAGGCTGAAGAGGCCATCCGTCGTGAA
ACGGAACGCCGCGCAGATGAAATTGTCCGTAAAATGGCAGAGAACAAACCTGACCTGCCG
GATGGCAAAACAGAGCAGGCTGTCAGGGAGATTGCCGGGCAGGAGCGTGACCGGGCTGCC
ATAACTGAACGGGAAGCCGCGCTGCCGGAGGGTGTACTGCGTGAACCTCAACGGGTGCGG
GAAGCTGTCAGGGAAATTGCCCGGGAAAATCTGCTGCAGGAGCGACTTCAGCAGATGGAG
CGGGATATGGTCCGCGACCTGCAGAAAGAGAAAACCCTGGGTGGAGACTGA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 1756 |
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| Protein Molecular Weight: | 192015 |
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| Protein Theoretical pI: | 6 |
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| PDB File: | 1P4D |
| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >Protein traI
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAADQRAD
LRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNMKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQAIRSELKTQGVLGLPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKSFAAEGTG
FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVMPLMER
VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
REIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK
QHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQERLQQME
RDMVRDLQKEKTLGGD |
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| References |
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| External Links: | |
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| General Reference: | - Bradshaw, H. D. Jr, Traxler, B. A., Minkley, E. G. Jr, Nester, E. W., Gordon, M. P. (1990). "Nucleotide sequence of the traI (helicase I) gene from the sex factor F." J Bacteriol 172:4127-4131. Pubmed: 2163400
- Frost, L. S., Ippen-Ihler, K., Skurray, R. A. (1994). "Analysis of the sequence and gene products of the transfer region of the F sex factor." Microbiol Rev 58:162-210. Pubmed: 7915817
- Jalajakumari, M. B., Manning, P. A. (1989). "Nucleotide sequence of the traD region in the Escherichia coli F sex factor." Gene 81:195-202. Pubmed: 2680768
- Penfold, S. S., Simon, J., Frost, L. S. (1996). "Regulation of the expression of the traM gene of the F sex factor of Escherichia coli." Mol Microbiol 20:549-558. Pubmed: 8736534
- Yoshioka, Y., Fujita, Y., Ohtsubo, E. (1990). "Nucleotide sequence of the promoter-distal region of the tra operon of plasmid R100, including traI (DNA helicase I) and traD genes." J Mol Biol 214:39-53. Pubmed: 2164585
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