Sensor protein zraS (P14377)

Identification
Name:Sensor protein zraS
Synonyms:Not Available
Gene Name:zraS
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Component
cell part
membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
molecular transducer activity
nucleoside binding
protein histidine kinase activity
protein kinase activity
purine nucleoside binding
signal transducer activity
transferase activity
transferase activity, transferring phosphorus-containing groups
two-component sensor activity
Process
biological regulation
cellular metabolic process
metabolic process
peptidyl-histidine phosphorylation
phosphate metabolic process
phosphorus metabolic process
phosphorylation
protein amino acid phosphorylation
regulation of biological process
regulation of cellular process
signal transduction
Gene Properties
Blattner:b4003
Gene OrientationClockwise
Centisome Percentage:90.52
Left Sequence End4199949
Right Sequence End4201346
Gene Sequence:
>1398 bp
ATGAAGCGACTTAAAAATGAACTCAATGCGCTGGTGAATCGGGGTGTCGACAGACATCTG
CGCCTCGCTGTAACCGGACTTAGCCGCAGCGGCAAAACAGCGTTTATCACCGCGATGGTT
AATCAGTTGCTTAATATTCACGCCGGAGCACGTTTGCCGCTGTTAAGTGCGGTGCGTGAA
GAGCGCCTGCTGGGCGTGAAACGCATTCCCCAGCGTGACTTTGGCATTCCGCGCTTCACC
TATGACGAAGGGCTGGCGCAGTTATATGGCGATCCACCCGCCTGGCCAACGCCAACGCGC
GGCGTCAGTGAAATCCGCCTGGCGCTACGTTTTAAATCGAATGATTCGCTGCTACGCCAC
TTTAAGGATACCTCCACGCTGTATCTGGAAATTGTGGATTATCCCGGCGAATGGTTGCTC
GACCTGCCGATGCTGGCGCAGGACTATTTAAGCTGGTCGCGCCAGATGACGGGCTTACTC
AATGGTCAGCGCGGAGAATGGTCGGCGAAATGGCGAATGATGAGCGAAGGGCTGGACCCG
CTAGCGCCTGCCGACGAAAACCGGCTGGCGGACATTGCCGCCGCGTGGACCGATTATCTC
CACCACTGTAAAGAGCAGGGGCTGCACTTTATTCAGCCTGGGCGCTTTGTCTTGCCGGGA
GATATGGCAGGTGCGCCCGCGCTGCAATTCTTCCCGTGGCCGGATGTCGATACCTGGGGC
GAGTCCAAACTGGCGCAGGCCGATAAGCATACCAATGCCGGAATGCTGCGCGAGCGCTTT
AATTATTACTGCGAGAAGGTGGTGAAGGGGTTCTATAAGAATCATTTTCTGCGCTTTGAC
CGCCAGATTGTGCTGGTGGATTGCCTGCAACCTCTCAACAGTGGGCCACAGGCATTTAAT
GATATGCGTCTGGCACTGACGCAGCTGATGCAAAGTTTTCACTACGGGCAGCGTACCCTG
TTCCGGCGTTTGTTTTCGCCGGTTATCGATAAGCTATTGTTTGCTGCCACTAAAGCGGAC
CATGTGACCATCGATCAGCACGCTAATATGGTTTCATTACTGCAACAACTGATTCAGGAT
GCCTGGCAAAATGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTT
CAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGT
AATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTG
CCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTG
ATGGATGTCGACAAACCACTACCGCATATTCGTCTTGATGCTGCGCTGGAATTTTTAATA
GGAGATAAATTGCGATGA
Protein Properties
Pfam Domain Function:
Protein Residues:465
Protein Molecular Weight:51031
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 15-35
  • 202-222
Protein Sequence:
>Sensor protein zraS
MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRASEADRQALLEKGNVLIRAL
ESGSRVGMGMRMHHVQQQALLEEMAGQPGVLWFAVTDAQGIIILHSDPDKVGRALYSPDE
MQKLKPEENSRWRLLGKTETTPALEVYRLFQPMSAPWRHGMHNMPRCNGKAVPQVDAQQA
IFIAVDASDLVATQSGEKRNTLIILFALATVLLASVLSFFWYRRYLRSRQLLQDEMKRKE
KLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQLAQVMAKEADRLNRVVSE
LLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRFTANDTLPEIQADPDRLTQV
LLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKGIAADQLDAIFTPYFTTKAEGT
GLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVNITRKDPQG
References
External Links:
ResourceLink
Uniprot ID:P14377
Uniprot Name:ZRAS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674904
Ecogene ID:EG10008
Ecocyc:EG10008
ColiBase:b4003
Kegg Gene:b4003
EchoBASE ID:EB0008
CCDB:ZRAS_ECOLI
BacMap:16131833
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Leonhartsberger, S., Huber, A., Lottspeich, F., Bock, A. (2001). "The hydH/G Genes from Escherichia coli code for a zinc and lead responsive two-component regulatory system." J Mol Biol 307:93-105. Pubmed: 11243806
  • Stoker, K., Reijnders, W. N., Oltmann, L. F., Stouthamer, A. H. (1989). "Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12." J Bacteriol 171:4448-4456. Pubmed: 2666400