Aerobic glycerol-3-phosphate dehydrogenase (P13035)

Identification
Name:Aerobic glycerol-3-phosphate dehydrogenase
Synonyms:Not Available
Gene Name:glpD
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0FAD ↔ 1.0Dihydroxyacetone phosphate + 1.0FADH2
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Quinone ↔ 1.0Dihydroxyacetone phosphate + 1.0Hydroquinone
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Glycerol 3-phosphate + 1.0Ubiquinone-8 → 1.0Dihydroxyacetone phosphate + 1.0Ubiquinol-8
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01473Dihydroxyacetone phosphateMetaboCard
ECMDB01248FADMetaboCard
ECMDB01197FADH2MetaboCard
ECMDB00126Glycerol 3-phosphateMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
glycerol-3-phosphate dehydrogenase complex
macromolecular complex
protein complex
Function
catalytic activity
glycerol-3-phosphate dehydrogenase activity
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, quinone or similar compound as acceptor
Process
alcohol metabolic process
alditol metabolic process
glycerol metabolic process
glycerol-3-phosphate metabolic process
metabolic process
oxidation reduction
polyol metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3426
Gene OrientationClockwise
Centisome Percentage:76.73
Left Sequence End3560036
Right Sequence End3561541
Gene Sequence:
>1506 bp
ATGGAAACCAAAGATCTGATTGTGATAGGGGGCGGCATCAATGGTGCTGGTATCGCGGCA
GACGCCGCTGGACGCGGTTTATCCGTGCTGATGCTGGAGGCGCAGGATCTCGCTTGCGCG
ACCTCTTCCGCCAGTTCAAAACTCATTCACGGTGGCCTGCGCTACCTTGAGCACTATGAA
TTCCGCCTGGTCAGCGAGGCGCTGGCTGAACGTGAAGTGCTGCTGAAAATGGCCCCGCAT
ATCGCCTTCCCGATGCGTTTTCGCCTGCCACATCGTCCGCATCTGCGCCCGGCGTGGATG
ATTCGCATTGGTCTGTTTATGTACGATCATCTGGGTAAACGCACCAGCTTGCCGGGATCA
ACTGGTTTGCGTTTTGGCGCAAATTCAGTGTTAAAACCGGAAATTAAGCGCGGATTCGAA
TATTCTGACTGTTGGGTAGACGACGCCCGTCTGGTACTCGCCAACGCCCAGATGGTGGTG
CGTAAAGGCGGCGAAGTGCTTACTCGGACTCGCGCCACCTCTGCTCGCCGCGAAAACGGC
CTGTGGATTGTGGAAGCGGAAGATATCGATACCGGCAAAAAATATAGCTGGCAAGCGCGC
GGCTTGGTTAACGCCACCGGCCCGTGGGTGAAACAGTTCTTCGACGACGGGATGCATCTG
CCTTCGCCTTATGGCATTCGCCTGATCAAAGGCAGCCATATTGTGGTGCCGCGCGTGCAT
ACCCAGAAGCAAGCCTACATTCTGCAAAACGAAGATAAACGTATTGTGTTCGTGATCCCG
TGGATGGACGAGTTTTCCATCATCGGCACTACCGATGTCGAGTACAAAGGCGATCCGAAA
GCGGTGAAGATTGAAGAGAGTGAAATCAATTACCTGCTGAATGTGTATAACACGCACTTT
AAAAAGCAGTTAAGCCGTGACGATATCGTCTGGACCTACTCCGGTGTGCGTCCGCTGTGT
GATGATGAGTCCGACTCGCCGCAGGCTATTACCCGTGATTACACCCTTGATATTCATGAT
GAAAATGGCAAAGCACCGCTGCTGTCGGTATTCGGCGGTAAGCTGACCACCTACCGAAAA
CTGGCGGAACATGCGCTGGAAAAACTAACGCCGTATTATCAGGGTATTGGCCCGGCATGG
ACGAAAGAGAGTGTGCTACCGGGTGGCGCCATTGAAGGCGACCGCGACGATTATGCCGCT
CGCCTGCGCCGCCGCTATCCGTTCCTGACTGAATCGCTGGCGCGTCATTACGCTCGCACT
TACGGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAA
GATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTC
CGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGAT
CAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCG
TCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:501
Protein Molecular Weight:56750
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Aerobic glycerol-3-phosphate dehydrogenase
METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSASSKLIHGGLRYLEHYE
FRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIRIGLFMYDHLGKRTSLPGS
TGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVVRKGGEVLTRTRATSARRENG
LWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDGMHLPSPYGIRLIKGSHIVVPRVH
TQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYKGDPKAVKIEESEINYLLNVYNTHF
KKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDYTLDIHDENGKAPLLSVFGGKLTTYRK
LAEHALEKLTPYYQGIGPAWTKESVLPGGAIEGDRDDYAARLRRRYPFLTESLARHYART
YGSNSELLLGNAGTVSDLGEDFGHEFYEAELKYLVDHEWVRRADDALWRRTKQGMWLNAD
QQSRVSQWLVEYTQQRLSLAS
References
External Links:
ResourceLink
Uniprot ID:P13035
Uniprot Name:GLPD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676616
Ecogene ID:EG10394
Ecocyc:EG10394
ColiBase:b3426
Kegg Gene:b3426
EchoBASE ID:EB0389
CCDB:GLPD_ECOLI
BacMap:16131300
General Reference:
  • Austin, D., Larson, T. J. (1991). "Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12." J Bacteriol 173:101-107. Pubmed: 1987111
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Choi, Y. L., Kawase, S., Nishida, T., Sakai, H., Komano, T., Kawamukai, M., Utsumi, R., Kohara, Y., Akiyama, K. (1988). "Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12." Nucleic Acids Res 16:7732. Pubmed: 3045764
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ye, S. Z., Larson, T. J. (1988). "Structures of the promoter and operator of the glpD gene encoding aerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12." J Bacteriol 170:4209-4215. Pubmed: 3045087