1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (P13016)

Identification
Name:1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Synonyms:
  • N-acetylmuramoyl-L-alanine amidase
Gene Name:ampD
Enzyme Class:
Biological Properties
General Function:Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function:Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety
Cellular Location:Cytoplasm
SMPDB Pathways:
  • 1,6-anhydro-<i>N</i>-acetylmuramic acid recycling PW002064
KEGG Pathways:Not Available
SMPDB Reactions:
1.0GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala+1.0Thumb → 1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate+1.0L-Ala-gamma-D-Glu-DAP-D-Ala
1.0GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala + 1.0Water → 1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + 1.0L-Ala-gamma-D-Glu-DAP-D-Ala
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptide + 1.0Water → 1.0L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptide + 1.0Water → 1.0L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + 1.0N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acid
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.01,6-Anhydrous-N-Acetylmuramyl-tetrapeptide + 1.0Water → 1.0L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanine + 1.01,6-Anhydro-N-acetylmuramate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.01,6-Anhydrous-N-Acetylmuramyl-tripeptide + 1.0Water → 1.0L-alanine-D-glutamate-meso-2,6-diaminoheptanedioate + 1.01,6-Anhydro-N-acetylmuramate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB205191,6-Anhydro-N-acetylmuramateMetaboCard
ECMDB211111,6-Anhydrous-N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB211121,6-Anhydrous-N-Acetylmuramyl-tripeptideMetaboCard
ECMDB21240L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
ECMDB21239L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
ECMDB21248N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
ECMDB21249N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
ECMDB21250N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b0110
Gene OrientationClockwise
Centisome Percentage:2.56
Left Sequence End118733
Right Sequence End119284
Gene Sequence:
>552 bp
ATGTTGTTAGAACAGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTCACCACATTACGAT
TGCCGCCCGGATGACGAAACACCCACCCTGCTGGTGGTGCACAATATTAGCCTGCCGCCA
GGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTATTGATCCGCAGGCA
CATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGT
GATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCT
CAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACC
GATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTG
ATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGAT
CGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAG
GAGACAACATGA
Protein Properties
Pfam Domain Function:
Protein Residues:183
Protein Molecular Weight:20536
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQA
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGT
DTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPDRKTDPGPAFDWARFRVLVSK
ETT
References
External Links:
ResourceLink
Uniprot ID:P13016
Uniprot Name:AMPD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21238958
Ecogene ID:EG10041
Ecocyc:EG10041
ColiBase:b0110
Kegg Gene:b0110
EchoBASE ID:EB0039
CCDB:AMPD_ECOLI
BacMap:16128103
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Honore, N., Nicolas, M. H., Cole, S. T. (1989). "Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer." Mol Microbiol 3:1121-1130. Pubmed: 2607970
  • Lindquist, S., Galleni, M., Lindberg, F., Normark, S. (1989). "Signalling proteins in enterobacterial AmpC beta-lactamase regulation." Mol Microbiol 3:1091-1102. Pubmed: 2691840
  • Whitchurch, C. B., Mattick, J. S. (1994). "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria." Gene 150:9-15. Pubmed: 7959070