Arginyl-tRNA synthetase (P11875)

Identification
Name:Arginyl-tRNA synthetase
Synonyms:
  • Arginine--tRNA ligase
  • ArgRS
Gene Name:argS
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Arg)+1.0tRNA(Arg) ↔ 1.0Thumb+1.0L-Arginyl-tRNA(Arg)+1.0Thumb+1.0L-Arginyl-tRNA(Arg)
1.0L-Arginine + 1.0Adenosine triphosphate + 1.0tRNA(Arg) + 1.0tRNA(Arg) ↔ 1.0Adenosine monophosphate + 1.0L-Arginyl-tRNA(Arg) + 1.0Pyrophosphate + 1.0L-Arginyl-tRNA(Arg)
ReactionCard
1.0Thumb+1.0Thumb+1.0tRNA(Arg) ↔ 1.0Thumb+1.0Thumb+1.0L-Arginyl-tRNA(Arg)
1.0Adenosine triphosphate + 1.0L-Arginine + 1.0tRNA(Arg) ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-Arginyl-tRNA(Arg)
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0tRNA(Arg)+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb+1.0L-arginyl-tRNA(Arg)
1.0L-Arginine + 1.0tRNA(Arg) + 1.0Adenosine triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.0Adenosine monophosphate + 1.0L-arginyl-tRNA(Arg)
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Arg) → 1.0Thumb+1.0Thumb+1.0L-arginyl-tRNA(Arg)
1.0Adenosine triphosphate + 1.0L-Arginine + 1.0tRNA(Arg) → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-arginyl-tRNA(Arg)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00517L-ArginineMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
arginine-tRNA ligase activity
ATP binding
binding
catalytic activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
arginyl-tRNA aminoacylation
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Blattner:b1876
Gene OrientationClockwise
Centisome Percentage:42.20
Left Sequence End1958086
Right Sequence End1959819
Gene Sequence:
>1734 bp
GTGAATATTCAGGCTCTTCTCTCAGAAAAAGTCCGTCAGGCCATGATTGCGGCAGGCGCG
CCTGCGGATTGCGAACCGCAGGTTCGTCAGTCAGCAAAAGTTCAGTTCGGCGACTATCAG
GCTAACGGCATGATGGCAGTTGCTAAAAAACTGGGTATGGCACCGCGACAATTAGCAGAG
CAGGTGCTGACTCATCTGGATCTTAACGGTATCGCCAGCAAAGTTGAGATCGCCGGTCCA
GGCTTTATCAACATTTTCCTTGATCCGGCATTCCTGGCTGAACATGTTCAGCAGGCGCTG
GCGTCCGATCGTCTCGGTGTTGCTACGCCAGAAAAACAGACCATTGTGGTTGACTACTCT
GCGCCAAACGTGGCGAAAGAGATGCATGTCGGTCACCTGCGCTCTACCATTATTGGTGAC
GCAGCAGTGCGTACTCTGGAGTTCCTCGGTCACAAAGTGATTCGCGCAAACCACGTCGGC
GACTGGGGCACTCAGTTCGGTATGCTGATTGCATGGCTGGAAAAGCAGCAGCAGGAAAAC
GCCGGTGAAATGGAGCTGGCTGACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTAC
GATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGT
GACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAG
ATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTC
TACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCAAAGCCAAAGGTCTGGCAGTAGAA
AGCGAAGGGGCGACCGTCGTATTCCTTGATGAGTTTAAAAACAAGGAAGGCGAACCGATG
GGCGTGATCATTCAGAAGAAAGATGGCGGCTATCTCTACACCACCACTGATATCGCCTGT
GCGAAATATCGTTATGAAACACTGCATGCCGATCGCGTGCTGTATTACATCGACTCCCGT
CAGCATCAACACCTGATGCAGGCATGGGCGATCGTCCGTAAAGCAGGCTATGTACCGGAA
TCCGTACCGCTGGAACACCACATGTTCGGCATGATGCTGGGTAAAGACGGCAAACCGTTC
AAAACCCGCGCGGGTGGTACAGTGAAACTGGCCGATCTGCTGGATGAAGCCCTGGAACGT
GCACGCCGTCTGGTGGCAGAAAAGAACCCGGATATGCCAGCCGACGAGCTGGAAAAACTG
GCTAACGCGGTTGGTATTGGTGCGGTGAAATATGCGGATCTCTCCAAAAACCGCACCACG
GACTACATCTTCGACTGGGACAACATGCTGGCGTTTGAGGGTAATACCGCGCCATACATG
CAGTATGCATACACGCGTGTATTGTCCGTGTTCCGTAAAGCAGAAATTGACGAAGAGCAA
CTGGCTGCAGCTCCGGTTATCATCCGTGAAGATCGTGAAGCGCAACTGGCAGCTCGCCTG
CTGCAGTTTGAAGAAACCCTCACCGTGGTTGCCCGTGAAGGCACGCCGCATGTAATGTGT
GCTTACCTGTACGATCTGGCCGGTCTGTTCTCTGGCTTCTACGAGCACTGCCCGATCCTC
AGCGCAGAAAACGAAGAAGTGCGTAACAGCCGTCTAAAACTGGCACAACTGACGGCGAAG
ACGCTGAAGCTGGGTCTGGATACGCTGGGTATTGAGACTGTAGAGCGTATGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:577
Protein Molecular Weight:64682
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Arginyl-tRNA synthetase
MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGMMAVAKKLGMAPRQLAE
QVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALASDRLGVATPEKQTIVVDYS
APNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVGDWGTQFGMLIAWLEKQQQEN
AGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQSGDEYFREMWRKLVDITMTQNQ
ITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKGLAVESEGATVVFLDEFKNKEGEPM
GVIIQKKDGGYLYTTTDIACAKYRYETLHADRVLYYIDSRQHQHLMQAWAIVRKAGYVPE
SVPLEHHMFGMMLGKDGKPFKTRAGGTVKLADLLDEALERARRLVAEKNPDMPADELEKL
ANAVGIGAVKYADLSKNRTTDYIFDWDNMLAFEGNTAPYMQYAYTRVLSVFRKAEIDEEQ
LAAAPVIIREDREAQLAARLLQFEETLTVVAREGTPHVMCAYLYDLAGLFSGFYEHCPIL
SAENEEVRNSRLKLAQLTAKTLKLGLDTLGIETVERM
References
External Links:
ResourceLink
Uniprot ID:P11875
Uniprot Name:SYR_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736522
Ecogene ID:EG10071
Ecocyc:EG10071
ColiBase:b1876
Kegg Gene:b1876
EchoBASE ID:EB0069
CCDB:SYR_ECOLI
BacMap:16129828
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Eriani, G., Dirheimer, G., Gangloff, J. (1989). "Isolation and characterization of the gene coding for Escherichia coli arginyl-tRNA synthetase." Nucleic Acids Res 17:5725-5736. Pubmed: 2668891
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040