Trk system potassium uptake protein trkA (P0AGI8)

Identification
Name:Trk system potassium uptake protein trkA
Synonyms:
  • K(+)-uptake protein trkA
Gene Name:trkA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in cation transmembrane transporter activity
Specific Function:Part of the constitutive potassium transport systems trkG and trkH. May regulate the transport activity of trkG and trkH systems. Binds to NAD+ and NADH
Cellular Location:Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04139PotassiumMetaboCard
GO Classification:
Function
binding
catalytic activity
cation transmembrane transporter activity
ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
metabolic process
monovalent inorganic cation transport
potassium ion transport
transport
Gene Properties
Blattner:b3290
Gene OrientationClockwise
Centisome Percentage:74.03
Left Sequence End3434540
Right Sequence End3435916
Gene Sequence:
>1377 bp
GTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCAT
CGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCGCAATTGGTACTGGT
CTGTTTCTTGGCATTGGCCCGGCGATTCAGATGGCGGGTCCGGCTGTATTGCTGGGCTAC
GGCGTCGCCGGGATCATCGCTTTCCTGATTATGCGCCAGCTTGGCGAAATGGTGGTTGAG
GAGCCGGTATCCGGTTCATTTGCCCACTTTGCCTATAAATACTGGGGACCGTTTGCGGGC
TTCCTCTCTGGCTGGAACTACTGGGTAATGTTCGTGCTGGTGGGAATGGCAGAGCTGACC
GCTGCGGGCATCTATATGCAGTACTGGTTCCCGGATGTTCCAACGTGGATTTGGGCTGCC
GCCTTCTTTATTATCATCAACGCCGTTAACCTGGTGAACGTGCGCTTATATGGCGAAACC
GAGTTCTGGTTTGCGTTGATTAAAGTGCTGGCAATCATCGGTATGATCGGCTTTGGCCTG
TGGCTGCTGTTTTCTGGTCACGGCGGCGAGAAAGCCAGTATCGACAACCTCTGGCGCTAC
GGTGGTTTCTTCGCCACCGGCTGGAATGGGCTGATTTTGTCGCTGGCGGTAATTATGTTC
TCCTTCGGCGGTCTGGAGCTGATTGGGATTACTGCCGCTGAAGCGCGCGATCCGGAAAAA
AGCATTCCAAAAGCGGTAAATCAGGTGGTGTATCGCATCCTGCTGTTTTACATCGGTTCA
CTGGTGGTTTTACTGGCGCTCTATCCGTGGGTGGAAGTGAAATCCAACAGTAGCCCGTTT
GTGATGATTTTCCATAATCTCGACAGCAACGTGGTAGCTTCTGCGCTGAACTTCGTCATT
CTGGTAGCATCGCTGTCAGTGTATAACAGCGGGGTTTACTCTAACAGCCGCATGCTGTTT
GGCCTTTCTGTGCAGGGTAATGCGCCGAAGTTTTTGACTCGCGTCAGCCGTCGCGGTGTG
CCGATTAACTCGCTGATGCTTTCCGGAGCGATCACTTCGCTGGTGGTGTTAATCAACTAT
CTGCTGCCGCAAAAAGCGTTTGGTCTGCTGATGGCGCTGGTGGTAGCAACGCTGCTGTTG
AACTGGATTATGATCTGTCTGGCGCATCTGCGTTTTCGTGCAGCGATGCGACGTCAGGGG
CGTGAAACACAGTTTAAGGCGCTGCTCTATCCGTTCGGCAACTATCTCTGCATTGCCTTC
CTCGGCATGATTTTGCTGCTGATGTGCACGATGGATGATATGCGCTTGTCAGCGATCCTG
CTGCCGGTGTGGATTGTATTCCTGTTTATGGCATTTAAAACGCTGCGTCGGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:458
Protein Molecular Weight:50367
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Trk system potassium uptake protein trkA
MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFDLRVVQGHGSHPRVLRE
AGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSPDYVRDADKLFHSDAVPID
HLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKAYYGGPLIGNALSTMREHMPH
IDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHIRAVMSELQRLEKPYKRIMLVGG
GNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQNTIVFFGDASDQELLAEEHIDQVDL
FIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRAYVDLVQGSVIDIAISPQQATISALLS
HVRKADIVGVSSLRRGVAEAIEAVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVM
IANDNLRIEQGDHVIMFLTDKKFITDVERLFQPSPFFL
References
External Links:
ResourceLink
Uniprot ID:P0AGI8
Uniprot Name:TRKA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651239
Ecogene ID:EG11019
Ecocyc:EG11019
ColiBase:b3290
Kegg Gene:b3290
EchoBASE ID:EB1012
CCDB:TRKA_ECOLI
BacMap:16131169
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bossemeyer, D., Borchard, A., Dosch, D. C., Helmer, G. C., Epstein, W., Booth, I. R., Bakker, E. P. (1989). "K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane." J Biol Chem 264:16403-16410. Pubmed: 2674131
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mazel, D., Coic, E., Blanchard, S., Saurin, W., Marliere, P. (1997). "A survey of polypeptide deformylase function throughout the eubacterial lineage." J Mol Biol 266:939-949. Pubmed: 9086272
  • Meinnel, T., Blanquet, S. (1994). "Characterization of the Thermus thermophilus locus encoding peptide deformylase and methionyl-tRNA(fMet) formyltransferase." J Bacteriol 176:7387-7390. Pubmed: 7961514
  • Schlosser, A., Hamann, A., Bossemeyer, D., Schneider, E., Bakker, E. P. (1993). "NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Mol Microbiol 9:533-543. Pubmed: 8412700