Phosphoribosylaminoimidazole carboxylase catalytic subunit (P0AG18)

Identification
Name:Phosphoribosylaminoimidazole carboxylase catalytic subunit
Synonyms:
  • AIR carboxylase
  • AIRC
Gene Name:purE
Enzyme Class:
Biological Properties
General Function:Involved in 5-(carboxyamino)imidazole ribonucleotide mutase activity
Specific Function:This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition
Cellular Location:Not Available
SMPDB Pathways:
  • purine nucleotides de novo biosynthesis PW000910
  • purine nucleotides de novo biosynthesis 1435709748 PW000960
  • purine nucleotides de novo biosynthesis 2 PW002033
KEGG Pathways:
KEGG Reactions:
1.05-Carboxyamino-1-(5-phospho-D-ribosyl)imidazole+1.0Thumb ↔ 1.0Thumb
1.05-Carboxyamino-1-(5-phospho-D-ribosyl)imidazole + 1.05-carboxyamino-1-(5-phospho-D-ribosyl)imidazole ↔ 1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate ↔ 1.05-Aminoimidazole ribonucleotide + 1.0Carbon dioxide
ReactionCard
SMPDB Reactions:
1.0N5-Carboxyaminoimidazole ribonucleotide+1.0Thumb → 1.0Thumb
1.0N5-Carboxyaminoimidazole ribonucleotide + 1.0N5-Carboxyaminoimidazole ribonucleotide → 1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
ReactionCard
1.0Thumb → 1.0Thumb
1.05-Phosphoribosyl-5-carboxyaminoimidazole → 1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
ReactionCard
Complex Reactions:
1.0Thumb ↔ 1.0Thumb
1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate ↔ 1.05-Phosphoribosyl-5-carboxyaminoimidazole
ReactionCard
1.0Thumb → 1.0Thumb
1.05-carboxyamino-1-(5-phospho-D-ribosyl)imidazole → 1.05-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB062735-Amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylateMetaboCard
ECMDB230205-carboxyamino-1-(5-phospho-D-ribosyl)imidazoleMetaboCard
ECMDB211785-Phosphoribosyl-5-carboxyaminoimidazoleMetaboCard
ECMDB01317N5-Carboxyaminoimidazole ribonucleotideMetaboCard
GO Classification:
Function
carbon-carbon lyase activity
carboxy-lyase activity
catalytic activity
lyase activity
phosphoribosylaminoimidazole carboxylase activity
Process
'de novo' IMP biosynthetic process
cellular nitrogen compound metabolic process
IMP biosynthetic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
purine nucleoside monophosphate biosynthetic process
purine nucleotide biosynthetic process
purine nucleotide metabolic process
purine ribonucleoside monophosphate biosynthetic process
Gene Properties
Blattner:b0523
Gene OrientationCounterclockwise
Centisome Percentage:11.89
Left Sequence End551814
Right Sequence End552323
Gene Sequence:
>510 bp
ATGTCTTCCCGCAATAATCCGGCGCGTGTCGCCATCGTGATGGGGTCCAAAAGCGACTGG
GCTACCATGCAGTTCGCCGCCGAAATCTTCGAAATCCTGAATGTCCCGCACCACGTTGAA
GTGGTTTCTGCTCACCGCACCCCCGATAAACTGTTCAGCTTCGCCGAAAGCGCCGAAGAG
AACGGTTATCAGGTGATTATTGCGGGCGCAGGCGGCGCAGCGCATCTGCCAGGCATGATT
GCCGCCAAAACGCTGGTGCCGGTGCTGGGCGTGCCAGTACAGAGCGCCGCACTGAGCGGT
GTCGATAGCCTCTACTCCATCGTACAAATGCCGCGCGGCATTCCGGTGGGTACGCTGGCG
ATTGGTAAAGCTGGCGCGGCAAACGCGGCGTTACTGGCAGCACAAATTCTTGCGACTCAT
GATAAAGAACTGCACCAGCGTCTGAATGACTGGCGCAAAGCCCAGACCGACGAAGTGCTG
GAAAACCCGGACCCGCGAGGTGCGGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:169
Protein Molecular Weight:17780
Protein Theoretical pI:7
PDB File:1QCZ
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphoribosylaminoimidazole carboxylase catalytic subunit
MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSAHRTPDKLFSFAESAEE
NGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVDSLYSIVQMPRGIPVGTLA
IGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVLENPDPRGAA
References
External Links:
ResourceLink
Uniprot ID:P0AG18
Uniprot Name:PUR6_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674660
PDB ID:1QCZ
Ecogene ID:EG10793
Ecocyc:EG10793
ColiBase:b0523
Kegg Gene:b0523
EchoBASE ID:EB0786
CCDB:PUR6_ECOLI
BacMap:16128507
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mathews, I. I., Kappock, T. J., Stubbe, J., Ealick, S. E. (1999). "Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway." Structure 7:1395-1406. Pubmed: 10574791
  • Meyer, E., Leonard, N. J., Bhat, B., Stubbe, J., Smith, J. M. (1992). "Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway." Biochemistry 31:5022-5032. Pubmed: 1534690
  • Tiedeman, A. A., Keyhani, J., Kamholz, J., Daum, H. A. 3rd, Gots, J. S., Smith, J. M. (1989). "Nucleotide sequence analysis of the purEK operon encoding 5'-phosphoribosyl-5-aminoimidazole carboxylase of Escherichia coli K-12." J Bacteriol 171:205-212. Pubmed: 2464576
  • Watanabe, W., Sampei, G., Aiba, A., Mizobuchi, K. (1989). "Identification and sequence analysis of Escherichia coli purE and purK genes encoding 5'-phosphoribosyl-5-amino-4-imidazole carboxylase for de novo purine biosynthesis." J Bacteriol 171:198-204. Pubmed: 2644189