ECMDB: Hydrogenase-4 component E (P0AEW1)

Identification

Name:

Hydrogenase-4 component E

Synonyms:

Not Available

Gene Name:

hyfE

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in oxidoreductase activity, acting on NADH or NADPH

Specific Function:

Specific function unknown

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0

→

1.0

1.0Formic acid + 1.0Hydrogen ion → 1.0Carbon dioxide + 1.0Hydrogen (gas)
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB04030	Carbon dioxide	MetaboCard
ECMDB00142	Formic acid	MetaboCard
ECMDB01362	Hydrogen (gas)	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on NADH or NADPH
Process
ATP synthesis coupled electron transport
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
oxidation reduction
respiratory electron transport chain

Gene Properties

Blattner:

b2485

Gene Orientation

Clockwise

Centisome Percentage:

56.13

Left Sequence End

2604284

Right Sequence End

2604934

Gene Sequence:

>651 bp
ATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATT
TATAAGATGGTGAGCGGGGACAAGATGGACGTGAAGATGAATATCTTTCAACGCTTGTGG
GAGACGTTACGCCATCTGTTCTGGTCAGATAAACAGACTGAGGCTTATAAACTTCTGTTC
AATTTCGTGAATAACCAGACTGGCAACATCAACGCCTCAGAATACTTTACTGGGGCTATC
AACGAGAATGAGAGAGAAAAGTTTATCAATAGCCTGGAATTATTCAATAAACTTAAAACA
TGCGCAAAAAATCCGGATGAGTTGGTCGCAAAGGGCAATATGCGCTGGGTCGCCCAGACC
TTCGGGGATATCGAGTTAAGTGTCACTTTTTTCATTGAAAAGAATAAGATATGTACTCAG
ACGTTGCAGCTGCATAAGGGCCAAGGTAACTTGGGCGTTGATCTTAGAAAGGCTTACCTT
CCCGGCGTTGACATGAGGGATTGTTACCTTGGTAAAAAAACAATGAAAGGTAGCAATGAT
ATCCTTTATGAGAGACCTGGGTGGAATGCTAACCTGGGCGTGCTACCCCGGACGGTGCTA
CCCCGGACGGTGCTAACCCGGACGGTGCTAACCTGGACGGTGCTACCGTGA

Protein Properties

Pfam Domain Function:

Oxidored_q2 (PF00420 )

Protein Residues:

216

Protein Molecular Weight:

23361

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

4-24
39-59
60-80
93-113
123-143
151-171
176-196

Protein Sequence:

>Hydrogenase-4 component E
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIW
SASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPM
ATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENGSHLVLALLAWRAPELVEIG
IATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG

References

External Links:

Resource	Link
Uniprot ID:	P0AEW1
Uniprot Name:	HYFE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674305
Ecogene ID:	EG14213
Ecocyc:	EG14213
ColiBase:	b2485
Kegg Gene:	b2485
EchoBASE ID:	EB3965
CCDB:	HYFE_ECOLI
BacMap:	16130410

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837