ECMDB

Identification

Name:

Sodium/glutamate symport carrier protein

Synonyms:

Glutamate permease

Gene Name:

gltS

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in glutamate:sodium symporter activity

Specific Function:

Catalyzes the sodium-dependent uptake of extracellular glutamate

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

D-Glutamine and D-glutamate metabolism PW000769
Glutathione metabolism PW000833
L-glutamate metabolism PW000789
Porphyrin metabolism PW000936
glutathione metabolism II PW001927
glutathione metabolism III PW002018
inner membrane transport PW000786
peptidoglycan biosynthesis I PW000906
peptidoglycan biosynthesis I 2 PW002062

KEGG Pathways:

D-Glutamine and D-glutamate metabolism ec00471
Glutathione metabolism ec00480

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590

Metabolites:

ECMDB ID	Name	View
ECMDB03339	D-Glutamic acid	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB02908	Sodium	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
glutamate:sodium symporter activity
organic acid transmembrane transporter activity
organic acid:sodium symporter activity
sodium:amino acid symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
L-glutamate transport
organic anion transport
transport

Gene Properties

Blattner:

b3653

Gene Orientation

Counterclockwise

Centisome Percentage:

82.45

Left Sequence End

3825483

Right Sequence End

3826688

Gene Sequence:

>1206 bp
ATGCGTGAAGCCTTTATTTGTGACGGAATTCGTACGCCAATTGGTCGCTACGGCGGGGCA
TTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGA
AACCCGCGTCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCG
GGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGT
GTTTCCGGCACAACCATTAACCGCTTGTGTGGTTCCGGGCTGGACGCACTGGGGTTTGCC
GCACGGGCGATTAAAGCGGGCGATGGCGATTTGCTGATCGCCGGTGGCGTGGAGTCAATG
TCACGGGCACCGTTTGTTATGGGCAAGGCAGCCAGTGCATTTTCTCGTCAGGCTGAGATG
TTCGATACCACTATTGGCTGGCGATTTGTGAACCCGCTCATGGCTCAGCAATTTGGAACT
GACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGAT
CAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATT
CTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATA
CAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCA
CCATTTCGTGCCAATGGGGTGATTACCGCAGGCAATGCTTCCGGGGTGAATGACGGAGCC
GCTGCGTTGATTATTGCCAGTGAACAGATGGCAGCAGCGCAAGGACTGACACCGCGGGCG
CGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTG
CCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATT
GAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCT
GATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGA
ATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGT
TACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGT
GTTTGA

Protein Properties

Pfam Domain Function:

Glt_symporter (PF03616 )

Protein Residues:

401

Protein Molecular Weight:

42425

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

3-21
36-54
69-87
91-109
112-132
160-178
214-240
246-267
276-294
301-325
336-357
371-398

Protein Sequence:

>Sodium/glutamate symport carrier protein
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLVLKKSMGWEV
NFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLG
LDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEVAMACATFGLVLGGLIGGPVA
RYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
TAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELA
SLALPMLAILVVQTIFMALYAIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
TERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFAG

References

External Links:

Resource	Link
Uniprot ID:	P0AER8
Uniprot Name:	GLTS_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1742272
Ecogene ID:	EG10406
Ecocyc:	EG10406
ColiBase:	b3653
Kegg Gene:	b3653
EchoBASE ID:	EB0401
CCDB:	GLTS_ECOLI
BacMap:	16131524

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Deguchi, Y., Yamato, I., Anraku, Y. (1990). "Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B." J Biol Chem 265:21704-21708. Pubmed: 2254324
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kalman, M., Gentry, D. R., Cashel, M. (1991). "Characterization of the Escherichia coli K12 gltS glutamate permease gene." Mol Gen Genet 225:379-386. Pubmed: 2017136
Kalman, M., Murphy, H., Cashel, M. (1992). "The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12." Gene 110:95-99. Pubmed: 1544582

Sodium/glutamate symport carrier protein (P0AER8)