Sodium/glutamate symport carrier protein (P0AER8)

Identification
Name:Sodium/glutamate symport carrier protein
Synonyms:
  • Glutamate permease
Gene Name:gltS
Enzyme Class:Not Available
Biological Properties
General Function:Involved in glutamate:sodium symporter activity
Specific Function:Catalyzes the sodium-dependent uptake of extracellular glutamate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB03339D-Glutamic acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB02908SodiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
glutamate:sodium symporter activity
organic acid transmembrane transporter activity
organic acid:sodium symporter activity
sodium:amino acid symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
ion transport
L-glutamate transport
organic anion transport
transport
Gene Properties
Blattner:b3653
Gene OrientationCounterclockwise
Centisome Percentage:82.45
Left Sequence End3825483
Right Sequence End3826688
Gene Sequence:
>1206 bp
ATGCGTGAAGCCTTTATTTGTGACGGAATTCGTACGCCAATTGGTCGCTACGGCGGGGCA
TTATCAAGTGTTCGGGCTGATGATCTGGCTGCTATCCCTTTGCGGGAACTGCTGGTGCGA
AACCCGCGTCTCGATGCGGAGTGTATCGATGATGTGATCCTCGGCTGTGCTAATCAGGCG
GGAGAAGATAACCGTAACGTAGCCCGGATGGCGACTTTACTGGCGGGGCTGCCGCAGAGT
GTTTCCGGCACAACCATTAACCGCTTGTGTGGTTCCGGGCTGGACGCACTGGGGTTTGCC
GCACGGGCGATTAAAGCGGGCGATGGCGATTTGCTGATCGCCGGTGGCGTGGAGTCAATG
TCACGGGCACCGTTTGTTATGGGCAAGGCAGCCAGTGCATTTTCTCGTCAGGCTGAGATG
TTCGATACCACTATTGGCTGGCGATTTGTGAACCCGCTCATGGCTCAGCAATTTGGAACT
GACAGCATGCCGGAAACGGCAGAGAATGTAGCTGAACTGTTAAAAATCTCACGAGAAGAT
CAAGATAGTTTTGCGCTACGCAGTCAGCAACGTACGGCAAAAGCGCAATCCTCAGGCATT
CTGGCTGAGGAGATTGTTCCGGTTGTGTTGAAAAACAAGAAAGGTGTTGTAACAGAAATA
CAACATGATGAGCATCTGCGCCCGGAAACGACGCTGGAACAGTTACGTGGGTTAAAAGCA
CCATTTCGTGCCAATGGGGTGATTACCGCAGGCAATGCTTCCGGGGTGAATGACGGAGCC
GCTGCGTTGATTATTGCCAGTGAACAGATGGCAGCAGCGCAAGGACTGACACCGCGGGCG
CGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTG
CCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATT
GAACTGAACGAAGCGTTCGCGGCCCAGGCGTTGGGTGTACTACGCGAATTGGGGCTGCCT
GATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGA
ATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGT
TACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGT
GTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:401
Protein Molecular Weight:42425
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 3-21
  • 36-54
  • 69-87
  • 91-109
  • 112-132
  • 160-178
  • 214-240
  • 246-267
  • 276-294
  • 301-325
  • 336-357
  • 371-398
Protein Sequence:
>Sodium/glutamate symport carrier protein
MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLLVALALLVLKKSMGWEV
NFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVVGLLVMQNAIGIGMASLLG
LDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEVAMACATFGLVLGGLIGGPVA
RYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLIETIALIAICLTVGKIVAQLLAG
TAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVSVLGNVSLSLFLAMALMGLKLWELA
SLALPMLAILVVQTIFMALYAIFVTWRMMGKNYDAAVLAAGHCGFGLGATPTAIANMQAI
TERFGPSHMAFLVVPMVGAFFIDIVNALVIKLYLMLPIFAG
References
External Links:
ResourceLink
Uniprot ID:P0AER8
Uniprot Name:GLTS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742272
Ecogene ID:EG10406
Ecocyc:EG10406
ColiBase:b3653
Kegg Gene:b3653
EchoBASE ID:EB0401
CCDB:GLTS_ECOLI
BacMap:16131524
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Deguchi, Y., Yamato, I., Anraku, Y. (1990). "Nucleotide sequence of gltS, the Na+/glutamate symport carrier gene of Escherichia coli B." J Biol Chem 265:21704-21708. Pubmed: 2254324
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kalman, M., Gentry, D. R., Cashel, M. (1991). "Characterization of the Escherichia coli K12 gltS glutamate permease gene." Mol Gen Genet 225:379-386. Pubmed: 2017136
  • Kalman, M., Murphy, H., Cashel, M. (1992). "The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12." Gene 110:95-99. Pubmed: 1544582