ECMDB: Ethanolamine ammonia-lyase heavy chain (P0AEJ6)

Identification

Name:

Ethanolamine ammonia-lyase heavy chain

Synonyms:

Ethanolamine ammonia-lyase large subunit

Gene Name:

eutB

Enzyme Class:

4.3.1.7

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Ethanolamine = acetaldehyde + NH(3)

Cellular Location:

Not Available

SMPDB Pathways:

ethanolamine PW002094

KEGG Pathways:

Glycerophospholipid metabolism ec00564

KEGG Reactions:

1.0

↔

1.0

1.0Ethanolamine ↔ 1.0Acetaldehyde + 1.0Ammonia
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Ethanolamine → 1.0Ammonium + 1.0Acetaldehyde
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Ethanolamine ↔ 1.0Acetaldehyde + 1.0Ammonia
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Ethanolamine → 1.0Acetaldehyde + 1.0Ammonium
ReactionCard

1.0

→

1.0

1.0Ethanolamine → 1.0Acetaldehyde + 1.0Ammonia
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00990	Acetaldehyde	MetaboCard
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB00149	Ethanolamine	MetaboCard

GO Classification:

Function
ammonia-lyase activity
carbon-nitrogen lyase activity
catalytic activity
ethanolamine ammonia-lyase activity
lyase activity
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process

Gene Properties

Blattner:

b2441

Gene Orientation

Counterclockwise

Centisome Percentage:

55.08

Left Sequence End

2555340

Right Sequence End

2556701

Gene Sequence:

>1362 bp
ATGTCTTTTGATTTAATCATTAAAAACGGCACCGTTATTTTAGAAAACGAAGCTCGCGTT
GTAGATATCGCCGTTAAAGGCGGAAAAATTGCTGCTATCGGTCAGGATCTGGGCGATGCA
AAAGAAGTTATGGATGCGTCTGGTCTGGTGGTTTCGCCGGGCATGGTTGATGCGCACACC
CATATTTCTGAACCGGGTCGTAGCCACTGGGAAGGTTATGAAACCGGTACTCGCGCAGCG
GCAAAAGGTGGTATCACCACCATGATCGAAATGCCGCTCAACCAGCTGCCTGCAACGGTT
GACCGCGCTTCAATTGAACTGAAGTTCGATGCCGCTAAAGGCAAGCTGACTATTGATGCG
GCACAACTCGGTGGCCTGGTGTCTTACAACATCGACCGTCTGCATGAGCTGGATGAAGTG
GGCGTTGTCGGCTTCAAATGCTTCGTTGCGACCTGTGGCGATCGCGGTATCGACAACGAC
TTCCGTGATGTAAACGACTGGCAGTTCTTCAAAGGTGCGCAGAAGCTGGGCGAACTGGGT
CAGCCGGTGCTGGTGCACTGCGAAAACGCGCTGATTTGTGACGAACTGGGCGAAGAAGCG
AAGCGTGAAGGTCGCGTAACCGCTCATGACTATGTGGCTTCGCGTCCGGTATTTACCGAA
GTGGAAGCAATTCGCCGCGTACTGTATCTGGCGAAAGTTGCTGGTTGCCGTCTGCACGTT
TGCCACGTCAGCAGCCCGGAAGGTGTTGAGGAAGTGACTCGTGCACGTCAGGAAGGTCAG
GACGTTACTTGTGAATCCTGCCCGCATTACTTTGTACTGGATACCGATCAGTTCGAAGAA
ATCGGTACTCTGGCGAAGTGTTCACCGCCGATCCGCGATCTGGAAAACCAGAAAGGCATG
TGGGAAAAACTGTTTAACGGTGAAATCGACTGCCTGGTTTCCGACCACTCTCCATGCCCG
CCGGAAATGAAAGCCGGTAACATCATGAAAGCATGGGGCGGTATCGCCGGTCTGCAAAGC
TGCATGGACGTGATGTTCGATGAAGCGGTACAGAAACGCGGTATGTCTCTGCCAATGTTC
GGCAAATTAATGGCGACTAACGCAGCAGATATTTTCGGTCTGCAGCAAAAAGGCCGTATC
GCCCCAGGAAAAGATGCCGACTTCGTCTTCATTCAGCCGAATAGCAGCTATGTTCTTACC
AATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCG
CGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCT
GTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAA

Protein Properties

Pfam Domain Function:

EutB (PF06751 )

Protein Residues:

453

Protein Molecular Weight:

49403

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Ethanolamine ammonia-lyase heavy chain
MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQERVAAKQVLSEMTVADIR
NNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSE
VVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEG
LSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAP
GGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANF
GADQVTMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMG
CDCCYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLN
LRPSPEFERWLESMGIMANGRLTKRAGDPSLFF

References

External Links:

Resource	Link
Uniprot ID:	P0AEJ6
Uniprot Name:	EUTB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674650
Ecogene ID:	EG50006
Ecocyc:	EG50006
ColiBase:	b2441
Kegg Gene:	b2441
EchoBASE ID:	EB4299
CCDB:	EUTB_ECOLI
BacMap:	90111437

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Faust, L. R., Connor, J. A., Roof, D. M., Hoch, J. A., Babior, B. M. (1990). "Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium." J Biol Chem 265:12462-12466. Pubmed: 2197274
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837