Succinyl-diaminopimelate desuccinylase (P0AED7)

Identification
Name:Succinyl-diaminopimelate desuccinylase
Synonyms:
  • SDAP desuccinylase
  • N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase
Gene Name:dapE
Enzyme Class:
Biological Properties
General Function:Involved in metallopeptidase activity
Specific Function:Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Water + 1.0N-Succinyl-L,L-2,6-diaminopimelate ↔ 1.0Diaminopimelic acid + 1.0Succinic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Succinyl-L,L-2,6-diaminopimelate + 1.0Water → 1.0Succinic acid + 1.0Diaminopimelic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Water + 1.0N-Succinyl-L,L-2,6-diaminopimelate ↔ 1.0Diaminopimelic acid + 1.0Succinic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01370Diaminopimelic acidMetaboCard
ECMDB12267N-Succinyl-L,L-2,6-diaminopimelateMetaboCard
ECMDB00254Succinic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
metallopeptidase activity
peptidase activity
peptidase activity, acting on L-amino acid peptides
protein binding
succinyl-diaminopimelate desuccinylase activity
Process
aspartate family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
lysine biosynthetic process
lysine biosynthetic process via diaminopimelate
lysine metabolic process
macromolecule metabolic process
metabolic process
protein metabolic process
proteolysis
Gene Properties
Blattner:b2472
Gene OrientationClockwise
Centisome Percentage:55.81
Left Sequence End2589629
Right Sequence End2590756
Gene Sequence:
>1128 bp
ATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGAT
CGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGG
ATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCAC
CTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATG
CAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGAT
ATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATT
TTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATG
ATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAG
ATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAAT
GCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAA
GGTCTGGTAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCG
AAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCG
CGTTACCTGATGGGCGTTGGTAAACCAGAAGACCTGGTTGAAGGCGTACGTCGTGGTATC
GATATGTTTGACTGCGTAATGCCAACCCGCAACGCCCGAAATGGTCATTTGTTCGTGACC
GATGGCGTGGTGAAAATCCGCAATGCGAAGTATAAGAGCGATACTGGCCCACTCGATCCT
GAGTGTGATTGCTACACCTGTCGCAATTATTCACGCGCTTACTTGCATCATCTTGACCGT
TGCAACGAAATATTAGGCGCGCGACTCAACACCATTCATAACCTTCGTTACTACCAGCGT
TTGATGGCGGGTTTACGCAAGGCTATTGAAGAGGGTAAATTAGAGAGCTTCGTAACTGAT
TTTTACCAGCGTCAGGGGCGAGAAGTACCACCTTTGAACGTTGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:375
Protein Molecular Weight:41269
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Succinyl-diaminopimelate desuccinylase
MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMDFADTQNFWAWRGQGET
LAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKGSLAAMVVAAERFVAQHPN
HTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLVGEPSSIEVVGDVVKNGRRGS
LTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIEWDQGNEFFPATSMQIANIQAGT
GSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLEKHQLRYTVDWWLSGQPFLTARGKL
VDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMGAQVVELGPVNATIHKINECVNAADLQ
LLARMYQRIMEQLVA
References
External Links:
ResourceLink
Uniprot ID:P0AED7
Uniprot Name:DAPE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674546
Ecogene ID:EG10208
Ecocyc:EG10208
ColiBase:b2472
Kegg Gene:b2472
EchoBASE ID:EB0204
CCDB:DAPE_ECOLI
BacMap:16130397
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bouvier, J., Richaud, C., Higgins, W., Bogler, O., Stragier, P. (1992). "Cloning, characterization, and expression of the dapE gene of Escherichia coli." J Bacteriol 174:5265-5271. Pubmed: 1644752
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lin, Y. K., Myhrman, R., Schrag, M. L., Gelb, M. H. (1988). "Bacterial N-succinyl-L-diaminopimelic acid desuccinylase. Purification, partial characterization, and substrate specificity." J Biol Chem 263:1622-1627. Pubmed: 3276674
  • Wu, B., Georgopoulos, C., Ang, D. (1992). "The essential Escherichia coli msgB gene, a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpE, is identical to dapE." J Bacteriol 174:5258-5264. Pubmed: 1644751
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837