Citrate carrier (P0AE74)

Identification
Name:Citrate carrier
Synonyms:
  • Citrate transporter
  • Citrate/succinate antiporter
Gene Name:citT
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00094Citric acidMetaboCard
ECMDB00254Succinic acidMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
cation transport
establishment of localization
ion transport
monovalent inorganic cation transport
sodium ion transport
transmembrane transport
transport
Gene Properties
Blattner:b0612
Gene OrientationCounterclockwise
Centisome Percentage:13.89
Left Sequence End644340
Right Sequence End645803
Gene Sequence:
>1464 bp
ATGTCTTTAGCAAAAGATAATATATGGAAACTATTGGCCCCACTGGTGGTGATGGGTGTC
ATGTTTCTTATCCCTGTCCCCGACGGTATGCCGCCGCAGGCATGGCATTACTTCGCTGTG
TTTGTGGCAATGATTGTCGGCATGATCCTCGAGCCAATTCCGGCAACAGCGATCAGTTTT
ATTGCGGTTACTATTTGCGTTATTGGCAGTAATTACCTGCTCTTTGATGCCAAAGAATTA
GCTGACCCAGCGTTTAATGCGCAAAAACAGGCGCTGAAATGGGGCCTGGCTGGTTTTTCC
AGCACCACGGTATGGCTGGTATTTGGCGCATTTATTTTTGCATTAGGGTATGAAGTTTCC
GGGTTAGGTCGTCGCATTGCCCTTTTCCTGGTGAAATTCATGGGCAAACGCACGCTGACG
TTGGGTTATGCGATTGTCATTATCGACATTCTGCTGGCACCGTTTACACCTTCCAACACC
GCGCGTACCGGGGGTACGGTTTTTCCGGTCATTAAAAACCTGCCGCCGTTGTTTAAATCA
TTCCCGAACGATCCGTCCGCGCGTCGTATTGGCGGCTATTTGATGTGGATGATGGTCATT
AGTACCAGTCTGAGTTCGTCCATGTTTGTCACCGGTGCGGCACCAAACGTGCTGGGTCTG
GAGTTCGTCAGCAAAATTGCCGGTATCCAGATTAGCTGGTTGCAGTGGTTCCTCTGCTTC
CTGCCGGTTGGGGTTATCTTGCTTATCATTGCGCCGTGGCTTTCCTACGTGCTGTACAAA
CCGGAAATCACACACAGTGAAGAAGTGGCAACCTGGGCGGGTGATGAACTGAAAACCATG
GGTGCGCTGACACGCAGAGAGTGGACACTGATTGGCCTTGTATTGCTCAGCTTAGGTTTG
TGGGTATTTGGCAGTGAAGTCATTAATGCTACTGCGGTTGGTCTGCTGGCAGTTTCGCTA
ATGCTGGCCCTGCACGTTGTGCCTTGGAAAGACATTACCCGCTATAACAGCGCATGGAAC
ACACTGGTCAACCTGGCAACTCTGGTTGTGATGGCTAACGGCCTGACTCGTTCTGGTTTT
ATTGACTGGTTCGCCGGTACCATGAGTACGCACCTGGAAGGATTCTCACCAAACGCAACG
GTGATTGTACTGGTTCTGGTGTTCTACTTTGCACACTACCTGTTTGCCAGCCTGTCTGCG
CACACCGCAACCATGCTGCCGGTTATTCTGGCCGTCGGTAAAGGTATTCCGGGCGTACCA
ATGGAACAACTGTGTATCCTGCTGGTGCTGTCTATCGGTATCATGGGCTGTCTGACGCCG
TATGCAACCGGTCCTGGGGTGATTATTTACGGCTGTGGCTATGTGAAATCAAAAGATTAC
TGGCGTCTTGGCGCAATCTTCGGGGTGATTTACATCTCTATGTTGCTGTTGGTTGGCTGG
CCGATTCTCGCCATGTGGAACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:487
Protein Molecular Weight:53092
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 11-31
  • 60-80
  • 95-115
  • 138-158
  • 190-210
  • 214-234
  • 237-257
  • 288-308
  • 309-329
  • 345-365
  • 379-399
  • 401-421
  • 424-444
  • 463-483
Protein Sequence:
>Citrate carrier
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
IDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN
References
External Links:
ResourceLink
Uniprot ID:P0AE74
Uniprot Name:CITT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062225
Ecogene ID:EG13538
Ecocyc:EG13538
ColiBase:b0612
Kegg Gene:b0612
CCDB:CITT_ECOLI
BacMap:16128595
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pos, K. M., Dimroth, P., Bott, M. (1998). "The Escherichia coli citrate carrier CitT: a member of a novel eubacterial transporter family related to the 2-oxoglutarate/malate translocator from spinach chloroplasts." J Bacteriol 180:4160-4165. Pubmed: 9696764