ECMDB: Acetolactate synthase isozyme 1 small subunit (P0ADF8)

Identification

Name:

Acetolactate synthase isozyme 1 small subunit

Synonyms:

Acetohydroxy-acid synthase I small subunit
AHAS-I
ALS-I

Gene Name:

ilvN

Enzyme Class:

2.2.1.6

Biological Properties

General Function:

Involved in amino acid binding

Specific Function:

2 pyruvate = 2-acetolactate + CO(2)

Cellular Location:

Not Available

SMPDB Pathways:

2-Oxopent-4-enoate metabolism PW001890
2-Oxopent-4-enoate metabolism 2 PW002035
Pantothenate and CoA biosynthesis PW000828
Valine Biosynthesis PW000812
isoleucine biosynthesis PW000818

KEGG Pathways:

Butanoate metabolism ec00650
C5-Branched dibasic acid metabolism ec00660
Metabolic pathways eco01100
Pantothenate and CoA biosynthesis ec00770
Valine, leucine and isoleucine biosynthesis ec00290

KEGG Reactions:

1.0

→

1.0

1.02-Ketobutyric acid + 1.0Hydrogen ion + 1.0Pyruvic acid → 1.02-Aceto-2-hydroxy-butyrate + 1.0Carbon dioxide
ReactionCard

1.0

2.0

→

1.0

1.0Hydrogen ion + 2.0Pyruvic acid → 1.0(S)-2-Acetolactate + 1.0Carbon dioxide
ReactionCard

1.0

↔

2.0

1.02-Acetolactate + 1.0Carbon dioxide ↔ 2.0Pyruvic acid
ReactionCard

1.0

↔

2.0

1.0(S)-2-Acetolactate + 1.0Carbon dioxide ↔ 2.0Pyruvic acid
ReactionCard

2.0

→

1.0

2.0Pyruvic acid → 1.02-Acetolactate + 1.0Carbon dioxide
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Pyruvic acid → 1.0Carbon dioxide + 1.02-Acetolactate
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.02-Ketobutyric acid + 1.0Hydrogen ion + 1.0Pyruvic acid → 1.02-Aceto-2-hydroxy-butyrate + 1.0Carbon dioxide
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB06855	(S)-2-Acetolactate	MetaboCard
ECMDB04001	2-Aceto-2-hydroxy-butyrate	MetaboCard
ECMDB04002	2-Acetolactate	MetaboCard
ECMDB00005	2-Ketobutyric acid	MetaboCard
ECMDB04030	Carbon dioxide	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00243	Pyruvic acid	MetaboCard
ECMDB01372	Thiamine pyrophosphate	MetaboCard

GO Classification:

Function
acetolactate synthase activity
amino acid binding
binding
carboxylic acid binding
catalytic activity
transferase activity
transferase activity, transferring aldehyde or ketonic groups
Process
branched chain family amino acid biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process

Gene Properties

Blattner:

b3670

Gene Orientation

Counterclockwise

Centisome Percentage:

82.95

Left Sequence End

3848825

Right Sequence End

3849115

Gene Sequence:

>291 bp
ATGGCTGATTTGAGAAAAGCAGCGCGTGGTCGGGAATGCCAGGTAAGAATCCCTGGCGTA
TGTAATGGCAACCCTGAAACGTCTGTACTGGCACATATCCGGCTGACTGGATTGTGCGGC
ACCGGTACGAAACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAA
ATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGT
ATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGA

Protein Properties

Pfam Domain Function:

ACT (PF01842 )

Protein Residues:

Protein Molecular Weight:

11106

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Acetolactate synthase isozyme 1 small subunit
MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPIQDSDKSHIWLLVNDDQ
RLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ

References

External Links:

Resource	Link
Uniprot ID:	P0ADF8
Uniprot Name:	ILVN_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674684
Ecogene ID:	EG10502
Ecocyc:	EG10502
ColiBase:	b3670
Kegg Gene:	b3670
EchoBASE ID:	EB0497
CCDB:	ILVN_ECOLI
BacMap:	16131540

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
Friden, P., Donegan, J., Mullen, J., Tsui, P., Freundlich, M., Eoyang, L., Weber, R., Silverman, P. M. (1985). "The ilvB locus of Escherichia coli K-12 is an operon encoding both subunits of acetohydroxyacid synthase I." Nucleic Acids Res 13:3979-3993. Pubmed: 2989781
Friedrich, M. J., Kadner, R. J. (1987). "Nucleotide sequence of the uhp region of Escherichia coli." J Bacteriol 169:3556-3563. Pubmed: 3301805
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Wek, R. C., Hauser, C. A., Hatfield, G. W. (1985). "The nucleotide sequence of the ilvBN operon of Escherichia coli: sequence homologies of the acetohydroxy acid synthase isozymes." Nucleic Acids Res 13:3995-4010. Pubmed: 2989782