Phosphogluconate dehydratase (P0ADF6)

Identification
Name:Phosphogluconate dehydratase
Synonyms:
  • 6-phosphogluconate dehydratase
Gene Name:edd
Enzyme Class:
Biological Properties
General Function:Involved in phosphogluconate dehydratase activity
Specific Function:6-phospho-D-gluconate = 2-dehydro-3-deoxy-6- phospho-D-gluconate + H(2)O
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.06-Phosphogluconic acid ↔ 1.02-Keto-3-deoxy-6-phosphogluconic acid + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.06-Phosphogluconic acid → 1.02-Keto-3-deoxy-6-phosphogluconic acid + 1.0Water
ReactionCard
SMPDB Reactions:
1.0gluconate 6-phosphate → 1.0Thumb+1.0Thumb
1.0gluconate 6-phosphate → 1.0Water + 1.02-Keto-3-deoxy-6-phosphogluconic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.06-Phosphogluconic acid ↔ 1.02-Keto-3-deoxy-6-phosphogluconic acid + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.06-Phosphogluconic acid → 1.02-Keto-3-deoxy-6-phosphogluconic acid + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB013762-Keto-3-deoxy-6-phosphogluconic acidMetaboCard
ECMDB013166-Phosphogluconic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
phosphogluconate dehydratase activity
Process
carbohydrate metabolic process
cellular carbohydrate metabolic process
Entner-Doudoroff pathway
metabolic process
primary metabolic process
Gene Properties
Blattner:b1851
Gene OrientationCounterclockwise
Centisome Percentage:41.62
Left Sequence End1930817
Right Sequence End1932628
Gene Sequence:
>1812 bp
ATGAATCCACAATTGTTACGCGTAACAAATCGAATCATTGAACGTTCGCGCGAGACTCGC
TCTGCTTATCTCGCCCGGATAGAACAAGCGAAAACTTCGACCGTTCATCGTTCGCAGTTG
GCATGCGGTAACCTGGCACACGGTTTCGCTGCCTGCCAGCCAGAAGACAAAGCCTCTTTG
AAAAGCATGTTGCGTAACAATATCGCCATCATCACCTCCTATAACGACATGCTCTCCGCG
CACCAGCCTTATGAACACTATCCAGAAATCATTCGTAAAGCCCTGCATGAAGCGAATGCG
GTTGGTCAGGTTGCGGGCGGTGTTCCGGCGATGTGTGATGGTGTCACCCAGGGGCAGGAT
GGAATGGAATTGTCGCTGCTAAGCCGCGAAGTGATAGCGATGTCTGCGGCGGTGGGGCTG
TCCCATAACATGTTTGATGGTGCTCTGTTCCTCGGTGTGTGCGACAAGATTGTCCCGGGT
CTGACGATGGCAGCCCTGTCGTTTGGTCATTTGCCTGCGGTGTTTGTGCCGTCTGGACCG
ATGGCAAGCGGTTTGCCAAATAAAGAAAAAGTGCGTATTCGCCAGCTTTATGCCGAAGGT
AAAGTGGACCGCATGGCCTTACTGGAGTCAGAAGCCGCGTCTTACCATGCGCCGGGAACA
TGTACTTTCTACGGTACTGCCAACACCAACCAGATGGTGGTGGAGTTTATGGGGATGCAG
TTGCCAGGCTCTTCTTTTGTTCATCCGGATTCTCCGCTGCGCGATGCTTTGACCGCCGCA
GCTGCGCGTCAGGTTACACGCATGACCGGTAATGGTAATGAATGGATGCCGATCGGTAAG
ATGATCGATGAGAAAGTGGTGGTGAACGGTATCGTTGCACTGCTGGCGACCGGTGGTTCC
ACTAACCACACCATGCACCTGGTGGCGATGGCGCGCGCGGCCGGTATTCAGATTAACTGG
GATGACTTCTCTGACCTTTCTGATGTTGTACCGCTGATGGCACGTCTCTACCCGAACGGT
CCGGCCGATATTAACCACTTCCAGGCGGCAGGTGGCGTACCGGTTCTGGTGCGTGAACTG
CTCAAAGCAGGCCTGCTGCATGAAGATGTCAATACGGTGGCAGGTTTTGGTCTGTCTCGT
TATACCCTTGAACCATGGCTGAATAATGGTGAACTGGACTGGCGGGAAGGGGCGGAAAAA
TCACTCGACAGCAATGTGATCGCTTCCTTCGAACAACCTTTCTCTCATCATGGTGGGACA
AAAGTGTTAAGCGGTAACCTGGGCCGTGCGGTTATGAAAACCTCTGCCGTGCCGGTTGAG
AACCAGGTGATTGAAGCGCCAGCGGTTGTTTTTGAAAGCCAGCATGACGTTATGCCGGCC
TTTGAAGCGGGTTTGCTGGACCGCGATTGTGTCGTTGTTGTCCGTCATCAGGGGCCAAAA
GCGAACGGAATGCCAGAATTACATAAACTCATGCCGCCACTTGGTGTATTATTGGACCGG
TGTTTCAAAATTGCGTTAGTTACCGATGGACGACTCTCCGGCGCTTCAGGTAAAGTGCCG
TCAGCTATCCACGTAACACCAGAAGCCTACGATGGCGGGCTGCTGGCAAAAGTGCGCGAC
GGGGACATCATTCGTGTGAATGGACAGACAGGCGAACTGACGCTGCTGGTAGACGAAGCG
GAACTGGCTGCTCGCGAACCGCACATTCCTGACCTGAGCGCGTCACGCGTGGGAACAGGA
CGTGAATTATTCAGCGCCTTGCGTGAAAAACTGTCCGGTGCCGAACAGGGCGCAACCTGT
ATCACTTTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:603
Protein Molecular Weight:64639
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphogluconate dehydratase
MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGNLAHGFAACQPEDKASL
KSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQD
GMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGP
MASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQ
LPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGS
TNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVREL
LKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGT
KVLSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPK
ANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRD
GDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGATC
ITF
References
External Links:
ResourceLink
Uniprot ID:P0ADF6
Uniprot Name:EDD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736494
Ecogene ID:EG10257
Ecocyc:EG10257
ColiBase:b1851
Kegg Gene:b1851
EchoBASE ID:EB0253
CCDB:EDD_ECOLI
BacMap:16129804
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, A. T., Pearson, B. M., Dickinson, J. R., Lancashire, W. E. (1993). "Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway." Gene 130:155-156. Pubmed: 8344525
  • Egan, S. E., Fliege, R., Tong, S., Shibata, A., Wolf, R. E. Jr, Conway, T. (1992). "Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon." J Bacteriol 174:4638-4646. Pubmed: 1624451
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040