Low-affinity gluconate transporter (P0AC96)

Identification
Name:Low-affinity gluconate transporter
Synonyms:
  • Gluconate permease
  • Gnt-I system
Gene Name:gntU
Enzyme Class:Not Available
Biological Properties
General Function:Involved in gluconate transmembrane transporter activity
Specific Function:Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00625Gluconic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
carboxylic acid transmembrane transporter activity
gluconate transmembrane transporter activity
monocarboxylic acid transmembrane transporter activity
organic acid transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carboxylic acid transport
establishment of localization
gluconate transport
monocarboxylic acid transport
organic acid transport
transport
Gene Properties
Blattner:b4476
Gene OrientationCounterclockwise
Centisome Percentage:77.03
Left Sequence End3573744
Right Sequence End3575084
Gene Sequence:
>1341 bp
GTGACTACATTAACGCTTGTTTTAACAGCAGTAGGGTCTGTTTTACTGCTGCTGTTTTTA
GTCATGAAGGCGCGTATGCACGCTTTCCTGGCTTTAATGGTGGTGTCCATGGGGGCTGGC
CTTTTTTCTGGTATGCCGCTCGATAAAATCGCAGCGACGATGGAAAAAGGGATGGGAGGC
ACCCTCGGCTTCCTGGCGGTGGTTGTCGCCCTGGGAGCTATGTTTGGCAAGATCTTACAT
GAAACCGGCGCAGTCGATCAGATTGCCGTCAAAATGCTCAAATCCTTCGGTCACAGCCGC
GCGCATTATGCCATCGGCCTTGCGGGGCTGGTCTGTGCGCTACCGCTGTTCTTTGAAGTG
GCGATAGTTCTGCTGATTAGCGTTGCTTTCTCAATGGCGCGCCACACCGGTACGAACCTG
GTGAAGCTGGTAATCCCATTATTTGCAGGCGTGGCGGCAGCGGCGGCATTTCTGGTGCCT
GGACCAGCGCCGATGCTGCTGGCATCGCAGATGAATGCCGACTTTGGCTGGATGATCCTG
ATTGGCCTGTGTGCGGCAATTCCGGGAATGATTATTGCCGGGCCGCTGTGGGGTAACTTC
ATCAGCCGTTACGTTGAGCTGCATATTCCTGACGACATCAGCGAACCGCATCTCGGCGAA
GGCAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGCTGCCGCTGGTGCTGGTA
GGGCTGAAAACCATTGCCGCGCGTTTTGTGCCGGAAGGATCAACTGCTTACGAATGGTTT
GAGTTTATCGGTCATCCGTTTACCGCGATTCTGGTTGCTTGTCTGGTGGCGATTTACGGT
CTGGCAATGCGTCAGGGCATGCCAAAAGACAAAGTGATGGAGATTTGCGGTCACGCGCTG
CAACCGGCGGGGATCATTCTGCTGGTGATTGGTGCGGGCGGCGTGTTCAAACAGGTGCTG
GTTGACTCTGGCGTAGGTCCGGCACTGGGCGAAGCGTTAACCGGCATGGGCCTGCCGATT
GCTATCACCTGCTTCGTGCTGGCAGCTGCAGTGCGCATCATTCAGGGTTCTGCCACCGTA
GCCTGTTTAACGGCGGTAGGACTGGTGATGCCGGTTATTGAACAACTGAACTACTCCGGT
GCGCAAATGGCGGCGCTGTCGATTTGTATCGCTGGTGGTTCGATTGTTGTCAGCCACGTT
AACGACGCCGGTTTCTGGTTGTTCGGTAAATTTACCGGCGCGACCGAAGCCGAAACGCTG
AAAACCTGGACCATGATGGAAACCATCCTCGGCACTGTCGGTGCCATCGTTGGGATGATT
GCGTTCCAGCTGTTGAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:446
Protein Molecular Weight:46416
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 2-22
  • 27-47
  • 59-79
  • 110-130
  • 143-163
  • 177-197
  • 226-246
  • 262-282
  • 295-315
  • 331-351
  • 353-373
  • 388-408
  • 425-445
Protein Sequence:
>Low-affinity gluconate transporter
MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGG
TLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAHYAIGLAGLVCALPLFFEV
AIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMIL
IGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLV
GLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHAL
QPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIAITCFVLAAAVRIIQGSATV
ACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETL
KTWTMMETILGTVGAIVGMIAFQLLS
References
External Links:
ResourceLink
Uniprot ID:P0AC96
Uniprot Name:GNTU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676607
Ecogene ID:EG12631
Ecocyc:EG12631
ColiBase:b4476
Kegg Gene:b4476
CCDB:GNTU_ECOLI
BacMap:49176355
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Izu, H., Adachi, O., Yamada, M. (1997). "Gene organization and transcriptional regulation of the gntRKU operon involved in gluconate uptake and catabolism of Escherichia coli." J Mol Biol 267:778-793. Pubmed: 9135111
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293
  • Tong, S., Porco, A., Isturiz, T., Conway, T. (1996). "Cloning and molecular genetic characterization of the Escherichia coli gntR, gntK, and gntU genes of GntI, the main system for gluconate metabolism." J Bacteriol 178:3260-3269. Pubmed: 8655507
  • Yamada, M., Kawai, T., Izu, H. (1996). "Analysis of the Escherichia coli gntT and gntU genes and comparison of the products with their homologues." Biosci Biotechnol Biochem 60:1548-1550. Pubmed: 8987614