Identification
Name:Glycogen phosphorylase
Synonyms:Not Available
Gene Name:glgP
Enzyme Class:
Biological Properties
General Function:Involved in phosphorylase activity
Specific Function:Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Starch+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Alpha-D-glucose 1-phosphate+1.0Dextrin+1.0Thumb
1.0Glycogen + 1.0Phosphate → 1.0Alpha-D-glucose 1-phosphate + 1.0Dextrin + 1.0Dextrin
ReactionCard
Complex Reactions:
1.0branching glycogen+1.0Thumb1.0Thumb
1.0branching glycogen + 1.0Phosphate → 1.0Glucose 1-phosphate
ReactionCard
1.0Thumb+1.0Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211101,4-alpha-D-glucanMetaboCard
ECMDB21651DextrinMetaboCard
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB21341GlycogenMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
phosphorylase activity
pyridoxal phosphate binding
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3428
Gene OrientationCounterclockwise
Centisome Percentage:76.78
Left Sequence End3562157
Right Sequence End3564604
Gene Sequence:
>2448 bp
ATGAATGCTCCGTTTACATATTCATCGCCCACGCTTAGCGTAGAAGCTCTTAAGCACTCT
ATCGCTTACAAGCTGATGTTTACGATTGGAAAGGACCCGGTCGTCGCCAATAAACATGAA
TGGCTGAACGCAACGTTATTTGCTGTGCGCGATCGTCTCGTGGAGCGCTGGTTACGTTCA
AACCGTGCCCAGTTGTCGCAAGAAACTCGTCAGGTTTACTACCTGTCGATGGAGTTTTTG
ATTGGCCGTACGCTCTCCAACGCCATGTTGTCGCTAGGAATTTACGAAGATGTACAGGGC
GCACTGGAAGCGATGGGGTTAAATCTCGAAGAGCTGATTGATGAAGAAAATGACCCAGGC
CTCGGTAACGGTGGCCTGGGACGTCTGGCGGCTTGCTTCCTTGATTCTCTGGCGACGTTA
GGGTTGCCGGGGCGCGGTTACGGCATCCGCTATGACTACGGTATGTTCAAGCAGAACATC
GTTAACGGTAGCCAGAAAGAGTCGCCAGACTACTGGCTGGAATACGGTAACCCGTGGGAA
TTCAAACGCCACAACACGCGCTATAAAGTCCGTTTTGGCGGTCGCATTCAGCAGGAAGGT
AAAAAAACGCGCTGGATTGAAACCGAAGAGATTCTGGGAGTCGCTTACGATCAGATAATC
CCTGGTTACGACACCGACGCGACCAACACGCTGCGTTTGTGGAGTGCGCAAGCCAGTAGC
GAAATTAACCTCGGTAAATTCAACCAGGGTGACTACTTCGCGGCAGTGGAAGATAAAAAC
CACTCCGAGAACGTATCTCGCGTACTGTATCCGGATGACTCCACCTACTCCGGGCGTGAG
CTGCGCCTGCGTCAGGAATACTTCCTGGTTTCCTCGACCATTCAGGACATTTTAAGCCGC
CATTATCAGTTGCATAAAACCTACGATAACCTGGCGGATAAAATCGCGATTCATCTCAAT
GATACCCATCCGGTACTGTCGATTCCTGAGATGATGCGTCTGCTGATCGATGAGCACCAA
TTTAGCTGGGACGACGCGTTTGAGGTGTGTTGTCAGGTCTTCTCCTACACTAACCACACG
CTGATGAGCGAGGCGCTGGAAACCTGGCCGGTTGATATGCTGGGTAAAATTCTGCCGCGT
CACCTGCAGATCATCTTTGAAATCAACGACTATTTCCTGAAAACCTTGCAGGAACAGTAT
CCGAACGATACCGATCTGCTGGGACGGGCGTCGATCATTGATGAATCCAACGGTCGTCGT
GTGCGTATGGCCTGGCTGGCGGTTGTTGTGAGCCACAAAGTTAACGGTGTATCGGAACTG
CACTCTAATCTGATGGTGCAATCGTTGTTTGCCGACTTTGCGAAAATCTTCCCGGGTCGT
TTCACCAACGTCACCAACGGTGTGACGCCGCGTCGCTGGCTGGCGGTAGCGAACCCATCG
CTTTCAGCCGTGCTGGACGAACACCTGGGCCGTAACTGGCGCACCGACCTTAGCCTGCTT
AATGAGCTGCAACAACACTGTGATTTCCCAATGGTTAATCACGCTGTGCATCAGGCGAAG
CTGGAGAACAAAAAGCGTCTGGCAGAGTATATCGCCCAGCAGCTGAATGTGGTGGTGAAT
CCAAAGGCGTTGTTCGATGTACAAATCAAACGTATTCACGAATACAAACGTCAATTGATG
AATGTGTTGCATGTGATTACCCGCTATAACCGCATCAAGGCCGACCCGGATGCGAAGTGG
GTACCGCGCGTGAATATTTTTGGCGGTAAGGCGGCTTCGGCCTATTACATGGCGAAGCAC
ATTATTCATTTGATCAATGACGTAGCGAAAGTGATCAACAACGATCCGCAGATTGGCGAT
AAGCTGAAAGTCGTGTTCATCCCGAACTACAGCGTTAGCCTGGCGCAGTTGATCATTCCG
GCGGCAGATCTGTCTGAACAGATTTCGCTGGCAGGGACGGAAGCTTCCGGCACCAGTAAC
ATGAAGTTTGCGCTTAACGGTGCGCTGACTATCGGTACGTTGGACGGTGCGAATGTCGAG
ATGCTGGATCATGTCGGTGCTGACAATATCTTTATTTTTGGTAACACAGCGGAAGAAGTG
GAAGAACTGCGTCGTCAGGGCTACAAACCGCGTGAATACTACGAGAAAGATGAGGAGCTG
CATCAGGTGCTGACGCAAATCGGCAGCGGTGTATTCAGTCCGGAAGATCCGGGTCGCTAT
CGCGATCTGGTTGATTCGCTGATCAACTTCGGCGATCACTACCAGGTACTGGCGGATTAT
CGCAGCTATGTCGATTGTCAGGATAAAGTCGATGAACTCTACGAGCTTCAGGAAGAGTGG
ACCGCAAAAGCGATGCTGAACATTGCCAATATGGGCTACTTCTCTTCTGACCGTACTATC
AAAGAGTACGCCGATCATATCTGGCATATCGATCCGGTGAGATTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:815
Protein Molecular Weight:93172
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycogen phosphorylase
MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNATLFAVRDRLVERWLRS
NRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGALEAMGLNLEELIDEENDPG
LGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNIVNGSQKESPDYWLEYGNPWE
FKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQIIPGYDTDATNTLRLWSAQASS
EINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTYSGRELRLRQEYFLVSSTIQDILSR
HYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRLLIDEHQFSWDDAFEVCCQVFSYTNHT
LMSEALETWPVDMLGKILPRHLQIIFEINDYFLKTLQEQYPNDTDLLGRASIIDESNGRR
VRMAWLAVVVSHKVNGVSELHSNLMVQSLFADFAKIFPGRFTNVTNGVTPRRWLAVANPS
LSAVLDEHLGRNWRTDLSLLNELQQHCDFPMVNHAVHQAKLENKKRLAEYIAQQLNVVVN
PKALFDVQIKRIHEYKRQLMNVLHVITRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKH
IIHLINDVAKVINNDPQIGDKLKVVFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSN
MKFALNGALTIGTLDGANVEMLDHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEEL
HQVLTQIGSGVFSPEDPGRYRDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEW
TAKAMLNIANMGYFSSDRTIKEYADHIWHIDPVRL
References
External Links:
ResourceLink
Uniprot ID:P0AC86
Uniprot Name:PHSG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676614
Ecogene ID:EG10380
Ecocyc:EG10380
ColiBase:b3428
Kegg Gene:b3428
EchoBASE ID:EB0375
CCDB:PHSG_ECOLI
BacMap:16131302
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Choi, Y. L., Kawamukai, M., Utsumi, R., Sakai, H., Komano, T. (1989). "Molecular cloning and sequencing of the glycogen phosphorylase gene from Escherichia coli." FEBS Lett 243:193-198. Pubmed: 2645169
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kumar, A., Larsen, C. E., Preiss, J. (1986). "Biosynthesis of bacterial glycogen. Primary structure of Escherichia coli ADP-glucose:alpha-1,4-glucan, 4-glucosyltransferase as deduced from the nucleotide sequence of the glgA gene." J Biol Chem 261:16256-16259. Pubmed: 3097003
  • Romeo, T., Kumar, A., Preiss, J. (1988). "Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes." Gene 70:363-376. Pubmed: 2975249
  • Yu, F., Jen, Y., Takeuchi, E., Inouye, M., Nakayama, H., Tagaya, M., Fukui, T. (1988). "Alpha-glucan phosphorylase from Escherichia coli. Cloning of the gene, and purification and characterization of the protein." J Biol Chem 263:13706-13711. Pubmed: 3047129