Naphthoate synthase (P0ABU0)

Identification
Name:Naphthoate synthase
Synonyms:
  • Dihydroxynaphthoic acid synthase
  • DHNA synthase
Gene Name:menB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4- dihydroxy-2-naphthoyl-CoA (DHNA-CoA)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.02-Succinylbenzoyl-CoA → 1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Succinylbenzoyl-CoA ↔ 1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.01,4-dihydroxy-2-naphthoyl-CoA+1.0Thumb
1.02-Succinylbenzoyl-CoA + 1.0Hydrogen ion → 1.0Water + 1.01,4-dihydroxy-2-naphthoyl-CoA + 1.01,4-Dihydroxy-2-naphthoyl-CoA
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.02-Succinylbenzoyl-CoA → 1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Succinylbenzoyl-CoA ↔ 1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.04-(2-Carboxyphenyl)-4-oxobutanoyl-CoA → 1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200321,4-Dihydroxy-2-naphthoyl-CoAMetaboCard
ECMDB200522-Succinylbenzoyl-CoAMetaboCard
ECMDB231514-(2-Carboxyphenyl)-4-oxobutanoyl-CoAMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
1,4-dihydroxy-2-naphthoyl-CoA synthase activity
carbon-carbon lyase activity
catalytic activity
lyase activity
oxo-acid-lyase activity
Process
fat-soluble vitamin metabolic process
menaquinone biosynthetic process
menaquinone metabolic process
metabolic process
small molecule metabolic process
vitamin K metabolic process
vitamin metabolic process
Gene Properties
Blattner:b2262
Gene OrientationCounterclockwise
Centisome Percentage:51.17
Left Sequence End2373984
Right Sequence End2374841
Gene Sequence:
>858 bp
TTGCTGAAGTATCGCCTGATATCTGCTTTTGTGTTAATACCCGTCGTCATCGCGGCGTTG
TTTCTGTTGCCGCCGGTGGGGTTCGCCATTGTAACGCTGGTGGTCTGCATGCTGGCAGCG
TGGGAATGGGGACAGCTTAGCGGTTTTACCACTCGTTCGCAGCGAGTATGGTTGGCGGTG
TTATGCGGGTTATTGTTGGCGCTGATGCTTTTTCTGTTGCCGGAATATCACCGAAATATT
CATCAACCGCTGGTTGAAATCTCACTTTGGGCTTCGCTGGGTTGGTGGATTGTCGCGCTA
TTGCTGGTGCTGTTTTACCCAGGTTCCGCAGCAATCTGGCGTAACTCTAAAACATTGCGC
CTTATTTTTGGCGTGCTAACCATTGTTCCCTTCTTCTGGGGCATGCTGGCGTTACGGGCC
TGGCACTATGACGAGAATCATTACAGTGGCGCAATATGGCTGCTCTATGTCATGATCCTG
GTATGGGGCGCTGACTCCGGCGCATATATGTTTGGCAAATTGTTTGGTAAACATAAGCTG
GCACCGAAGGTTTCTCCGGGTAAAACCTGGCAAGGCTTTATCGGTGGACTCGCTACTGCA
GCGGTAATCTCATGGGGTTATGGCATGTGGGCGAATCTCGACGTCGCTCCCGTCACCTTA
CTCATTTGCTCTATTGTCGCAGCGTTAGCCTCAGTGCTCGGCGATCTGACCGAGAGTATG
TTTAAGCGTGAAGCAGGAATTAAGGACAGCGGTCATTTAATTCCAGGACACGGTGGTATT
TTAGATCGTATTGATAGCCTGACGGCTGCGGTACCGGTCTTTGCTTGCTTGTTGTTACTG
GTATTCAGGACGCTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:285
Protein Molecular Weight:31633
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Naphthoate synthase
MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQVRNAFRPLTVKEMIQAL
ADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSGVHHLNVLDFQRQIRTCPK
PVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVGSFDGGWGASYMARIVGQKKA
REIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWCREMLQNSPMALRCLKAALNADC
DGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQPDFSKFKRNP
References
External Links:
ResourceLink
Uniprot ID:P0ABU0
Uniprot Name:MENB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674368
Ecogene ID:EG11368
Ecocyc:EG11368
ColiBase:b2262
Kegg Gene:b2262
EchoBASE ID:EB1342
CCDB:MENB_ECOLI
BacMap:16130197
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sharma, V., Suvarna, K., Meganathan, R., Hudspeth, M. E. (1992). "Menaquinone (vitamin K2) biosynthesis: nucleotide sequence and expression of the menB gene from Escherichia coli." J Bacteriol 174:5057-5062. Pubmed: 1629162
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837