Carbonic anhydrase 1 (P0ABE9)

Identification
Name:Carbonic anhydrase 1
Synonyms:
  • Carbonate dehydratase 1
Gene Name:cynT
Enzyme Class:
Biological Properties
General Function:Involved in carbonate dehydratase activity
Specific Function:Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (cynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to catalyze the hydration of carbon dioxide and thus prevent depletion of cellular bicarbonate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Carbonic acid ↔ 1.0Carbon dioxide + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb
1.0Carbon dioxide + 1.0Water → 1.0Hydrogen carbonate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Carbon dioxide + 1.0Water → 1.0Hydrogen ion + 1.0Hydrogen carbonate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Carbon dioxide + 1.0Water ↔ 1.0Hydrogen ion + 1.0Hydrogen carbonate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB03538Carbonic acidMetaboCard
ECMDB00595Hydrogen carbonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
carbon-oxygen lyase activity
carbonate dehydratase activity
catalytic activity
cation binding
hydro-lyase activity
ion binding
lyase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
carbon utilization
Gene Properties
Blattner:b0339
Gene OrientationClockwise
Centisome Percentage:7.72
Left Sequence End358023
Right Sequence End358682
Gene Sequence:
>660 bp
GTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAA
GCCTTGTTTAAACAGCTGGCGACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCC
GACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGCCTGGCGATCTGTTCGTTATT
CGCAACGCGGGCAATATCGTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCG
GTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAAC
TGTGGCGCGATGACCGCCATTGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCC
CACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATGAGGCGCGCCCGCATTCCGAT
TTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTG
CAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGG
GTCTACGACATTGAAAGCGGCAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTG
CCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTACGCCAACCGACCGCAGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:219
Protein Molecular Weight:23764
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Carbonic anhydrase 1
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVI
RNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVS
HWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGW
VYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
References
External Links:
ResourceLink
Uniprot ID:P0ABE9
Uniprot Name:CYNT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674481
Ecogene ID:EG10176
Ecocyc:EG10176
ColiBase:b0339
Kegg Gene:b0339
EchoBASE ID:EB0173
CCDB:CYNT_ECOLI
BacMap:16128324
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Guilloton, M. B., Korte, J. J., Lamblin, A. F., Fuchs, J. A., Anderson, P. M. (1992). "Carbonic anhydrase in Escherichia coli. A product of the cyn operon." J Biol Chem 267:3731-3734. Pubmed: 1740425
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sung, Y. C., Fuchs, J. A. (1988). "Characterization of the cyn operon in Escherichia coli K12." J Biol Chem 263:14769-14775. Pubmed: 3049588