Formate hydrogenlyase subunit 2 (P0AAK1)

Identification
Name:Formate hydrogenlyase subunit 2
Synonyms:
  • FHL subunit 2
  • Hydrogenase-3 component B
Gene Name:hycB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in electron carrier activity
Specific Function:Probable electron transfer protein for hydrogenase 3
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Formic acid + 1.0Hydrogen ion → 1.0Carbon dioxide + 1.0Hydrogen (gas)
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
binding
electron carrier activity
iron-sulfur cluster binding
metal cluster binding
Gene Properties
Blattner:b2724
Gene OrientationCounterclockwise
Centisome Percentage:61.37
Left Sequence End2847260
Right Sequence End2847871
Gene Sequence:
>612 bp
GTGAAATTGAGATGGTTTGCCTTTTTGATTGTGTTATTAGCGGGTTGTTCATCAAAGCAT
GACTATACGAACCCGCCGTGGAACGCGAAAGTTCCGGTGCAACGTGCGATGCAGTGGATG
CCAATAAGCCAGAAAGCCGGTGCAGCCTGGGGCGTCGATCCACAATTGATCACGGCGATT
ATCGCTATCGAATCGGGTGGTAATCCGAACGCGGTGAGTAAATCGAATGCCATTGGTTTG
ATGCAGTTAAAAGCTTCAACCTCCGGACGTGATGTTTATCGCCGTATGGGCTGGAGTGGT
GAGCCGACGACCAGCGAGCTGAAAAATCCAGAGCGTAATATTTCAATGGGGGCGGCTTAC
CTGAATATTCTGGAAACCGGCCCGCTGGCAGGCATTGAAGATCCGAAGGTACTGCAATAT
GCGCTGGTGGTGTCATACGCTAACGGGGCAGGTGCGCTGCTACGGACTTTCTCGTCAGAT
CGGAAAAAGGCGATCAGCAAAATCAACGATTTGGATGCTGACGAGTTCCTCGAACACGTA
GCGCGAAATCACCCTGCGCCGCAGGCTCCGCGCTATATCTACAAACTTGAGCAGGCACTG
GACGCGATGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:203
Protein Molecular Weight:21873
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Formate hydrogenlyase subunit 2
MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEKESAPQLCHHCEDAPCA
VVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGSRPLDIPANANTPKAPPAP
PAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCPTKALHLVDNTDIARVSKRKR
ELTFNTDFGDLTLFQQAQSGEAK
References
External Links:
ResourceLink
Uniprot ID:P0AAK1
Uniprot Name:HYCB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674869
Ecogene ID:EG10475
Ecocyc:EG10475
ColiBase:b2724
Kegg Gene:b2724
EchoBASE ID:EB0470
CCDB:HYCB_ECOLI
BacMap:16130631
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bohm, R., Sauter, M., Bock, A. (1990). "Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Mol Microbiol 4:231-243. Pubmed: 2187144
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553