Cold-shock DEAD box protein A (P0A9P6)

Identification
Name:Cold-shock DEAD box protein A
Synonyms:
  • ATP-dependent RNA helicase deaD
  • Translation factor W2
Gene Name:deaD
Enzyme Class:
Biological Properties
General Function:Replication, recombination and repair
Specific Function:Acts in 50S ribosomal subunit biogenesis at low temperatures, acting after srmB. Has a helix-destabilizing activity; however it is unclear whether it requires ATP:according to PubMed:8552679, it does not require ATP, while according to PubMed:15196029 and PubMed:15554978, it requires ATP. Requires a helicase substrate with a single-stranded overhang. Plays a key role in optimal cell growth at low temperature and is required for normal cell division. When overexpressed suppresses cold-sensitive mutants of the rpsB gene (ribosomal protein S2) and of the smbA2 cold-sensitive mutant (pyrH, uridylate kinase). Can replace rhlB, another ATP-dependent RNA helicase, in the RNA degradosome in vitro. Stimulates translation, probably at the level of initiation, of some mRNAs
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATP-dependent helicase activity
ATPase activity
ATPase activity, coupled
binding
catalytic activity
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside binding
nucleoside-triphosphatase activity
purine nucleoside binding
pyrophosphatase activity
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1890 bp
ATGGCTGAATTCGAAACCACTTTTGCAGATCTGGGCCTGAAGGCTCCTATCCTTGAAGCC
CTTAACGATCTGGGTTACGAAAAACCATCTCCAATTCAGGCAGAGTGTATTCCACATCTG
CTGAATGGCCGCGACGTTCTGGGTATGGCCCAGACGGGGAGCGGAAAAACTGCAGCATTC
TCTTTACCTCTGTTGCAGAATCTTGATCCTGAGCTGAAAGCACCACAGATTCTGGTGCTG
GCACCGACCCGCGAACTGGCGGTACAGGTTGCTGAAGCAATGACGGATTTCTCTAAACAC
ATGCGCGGCGTAAATGTGGTTGCTCTGTACGGCGGCCAGCGTTATGACGTGCAATTACGC
GCCCTGCGTCAGGGGCCGCAGATCGTTGTCGGTACTCCGGGCCGTCTGCTGGACCACCTG
AAACGTGGCACTCTGGACCTCTCTAAACTGAGCGGTCTGGTTCTGGATGAAGCTGACGAA
ATGCTGCGCATGGGCTTCATCGAAGACGTTGAAACCATTATGGCGCAGATCCCGGAAGGT
CATCAGACCGCTCTGTTCTCTGCAACCATGCCGGAAGCGATTCGTCGCATTACCCGCCGC
TTTATGAAAGAGCCGCAGGAAGTGCGCATTCAGTCCAGCGTGACTACCCGTCCTGACATC
AGCCAGAGCTACTGGACTGTCTGGGGTATGCGCAAAAACGAAGCACTGGTACGTTTCCTG
GAAGCGGAAGATTTTGATGCGGCGATTATCTTCGTTCGTACCAAAAACGCGACTCTGGAA
GTGGCTGAAGCTCTTGAGCGTAACGGCTACAACAGCGCCGCGCTGAACGGTGACATGAAC
CAGGCGCTGCGTGAACAGACACTGGAACGCCTGAAAGATGGTCGTCTGGACATCCTGATT
GCGACCGACGTTGCAGCCCGTGGCCTGGACGTTGAGCGTATCAGCCTGGTAGTTAACTAC
GATATCCCGATGGATTCTGAGTCTTACGTTCACCGTATCGGTCGTACCGGTCGTGCGGGT
CGTGCTGGCCGCGCGCTGCTGTTCGTTGAGAACCGCGAGCGTCGTCTGCTGCGCAACATT
GAACGTACTATGAAGCTGACTATTCCGGAAGTAGAACTGCCGAACGCAGAACTGCTAGGC
AAACGCCGTCTGGAAAAATTCGCCGCTAAAGTACAGCAGCAGCTGGAAAGCAGCGATCTG
GATCAATACCGCGCACTGCTGAGCAAAATTCAGCCGACTGCTGAAGGTGAAGAGCTGGAT
CTCGAAACTCTGGCTGCGGCACTGCTGAAAATGGCACAGGGTGAACGTACTCTGATCGTA
CCGCCAGATGCGCCGATGCGTCCGAAACGTGAATTCCGTGACCGTGATGACCGTGGTCCG
CGCGATCGTAACGACCGTGGCCCGCGTGGTGACCGTGAAGATCGTCCGCGTCGTGAACGT
CGTGATGTTGGCGATATGCAGCTGTACCGCATTGAAGTGGGCCGCGATGATGGTGTTGAA
GTTCGTCATATCGTTGGTGCGATTGCTAACGAAGGCGACATCAGCAGCCGTTACATTGGT
AACATCAAGCTGTTTGCTTCTCACTCCACCATCGAACTGCCGAAAGGTATGCCGGGTGAA
GTGCTGCAACACTTTACGCGCACTCGCATTCTCAACAAGCCGATGAACATGCAGTTACTG
GGCGATGCACAGCCGCATACTGGCGGTGAGCGTCGTGGCGGTGGTCGTGGTTTCGGTGGC
GAACGTCGTGAAGGCGGTCGTAACTTCAGCGGTGAACGCCGTGAAGGTGGCCGTGGTGAT
GGTCGTCGTTTTAGCGGCGAACGTCGTGAAGGCCGCGCTCCGCGTCGTGATGATTCTACC
GGTCGTCGTCGTTTCGGTGGTGATGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:629
Protein Molecular Weight:70546
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cold-shock DEAD box protein A
MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGRDVLGMAQTGSGKTAAF
SLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMRGVNVVALYGGQRYDVQLR
ALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADEMLRMGFIEDVETIMAQIPEG
HQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRPDISQSYWTVWGMRKNEALVRFL
EAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALNGDMNQALREQTLERLKDGRLDILI
ATDVAARGLDVERISLVVNYDIPMDSESYVHRIGRTGRAGRAGRALLFVENRERRLLRNI
ERTMKLTIPEVELPNAELLGKRRLEKFAAKVQQQLESSDLDQYRALLSKIQPTAEGEELD
LETLAAALLKMAQGERTLIVPPDAPMRPKREFRDRDDRGPRDRNDRGPRGDREDRPRRER
RDVGDMQLYRIEVGRDDGVEVRHIVGAIANEGDISSRYIGNIKLFASHSTIELPKGMPGE
VLQHFTRTRILNKPMNMQLLGDAQPHTGGERRGGGRGFGGERREGGRNFSGERREGGRGD
GRRFSGERREGRAPRRDDSTGRRRFGGDA
References
External Links:
ResourceLink
Uniprot ID:P0A9P6
Uniprot Name:DEAD_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:87082221
CCDB:DEAD_ECOLI
General Reference:
  • Bizebard, T., Ferlenghi, I., Iost, I., Dreyfus, M. (2004). "Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases." Biochemistry 43:7857-7866. Pubmed: 15196029
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Charollais, J., Dreyfus, M., Iost, I. (2004). "CsdA, a cold-shock RNA helicase from Escherichia coli, is involved in the biogenesis of 50S ribosomal subunit." Nucleic Acids Res 32:2751-2759. Pubmed: 15148362
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jones, P. G., Mitta, M., Kim, Y., Jiang, W., Inouye, M. (1996). "Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli." Proc Natl Acad Sci U S A 93:76-80. Pubmed: 8552679
  • Lu, J., Aoki, H., Ganoza, M. C. (1999). "Molecular characterization of a prokaryotic translation factor homologous to the eukaryotic initiation factor eIF4A." Int J Biochem Cell Biol 31:215-229. Pubmed: 10216955
  • Peng, H. L., Hsieh, M. J., Zao, C. L., Chang, H. Y. (1994). "Nucleotide sequence and expression in Escherichia coli of the Klebsiella pneumoniae deaD gene." J Biochem 115:409-414. Pubmed: 8056751
  • Prud'homme-Genereux, A., Beran, R. K., Iost, I., Ramey, C. S., Mackie, G. A., Simons, R. W. (2004). "Physical and functional interactions among RNase E, polynucleotide phosphorylase and the cold-shock protein, CsdA: evidence for a 'cold shock degradosome'." Mol Microbiol 54:1409-1421. Pubmed: 15554978
  • Toone, W. M., Rudd, K. E., Friesen, J. D. (1991). "deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2." J Bacteriol 173:3291-3302. Pubmed: 2045359
  • Turner, A. M., Love, C. F., Alexander, R. W., Jones, P. G. (2007). "Mutational analysis of the Escherichia coli DEAD box protein CsdA." J Bacteriol 189:2769-2776. Pubmed: 17259309
  • Yamanaka, K., Ogura, T., Koonin, E. V., Niki, H., Hiraga, S. (1994). "Multicopy suppressors, mssA and mssB, of an smbA mutation of Escherichia coli." Mol Gen Genet 243:9-16. Pubmed: 8190075