ECMDB: Serine acetyltransferase (P0A9D4)

Identification

Name:

Serine acetyltransferase

Synonyms:

Gene Name:

cysE

Enzyme Class:

2.3.1.30

Biological Properties

General Function:

Involved in transferase activity

Specific Function:

Acetyl-CoA + L-serine = CoA + O-acetyl-L- serine

Cellular Location:

Cytoplasm

SMPDB Pathways:

Secondary Metabolites: cysteine biosynthesis from serine PW000977
Sulfur metabolism PW000922
cysteine biosynthesis PW000800

KEGG Pathways:

Cysteine and methionine metabolism ec00270
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Sulfur metabolism ec00920

KEGG Reactions:

1.0

↔

1.0

1.0Acetyl-CoA + 1.0L-Serine ↔ 1.0O-Acetylserine + 1.0Coenzyme A
ReactionCard

SMPDB Reactions:

1.0L-Serine

1.0

→

1.0

1.0L-Serine + 1.0Acetyl-CoA + 1.0L-Serine → 1.0Coenzyme A + 1.0O-Acetylserine
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Acetyl-CoA + 1.0L-Serine ↔ 1.0O-Acetylserine + 1.0Coenzyme A
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB00187	L-Serine	MetaboCard
ECMDB03011	O-Acetylserine	MetaboCard

GO Classification:

Component
cell part
cytoplasm
intracellular part
Function
acyltransferase activity
catalytic activity
O-acyltransferase activity
serine O-acetyltransferase activity
serine O-acyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
cysteine biosynthetic process from serine
L-serine metabolic process
metabolic process
serine family amino acid metabolic process

Gene Properties

Blattner:

b3607

Gene Orientation

Counterclockwise

Centisome Percentage:

81.47

Left Sequence End

3779764

Right Sequence End

3780585

Gene Sequence:

>822 bp
ATGCATGATGCAAACATCCGCGTTGCCATCGCGGGAGCCGGGGGGCGTATGGGCCGCCAG
TTGATTCAGGCGGCGCTGGCATTAGAGGGCGTGCAGTTGGGCGCTGCGCTGGAGCGTGAA
GGATCTTCTTTACTGGGCAGCGACGCCGGTGAGCTGGCCGGAGCCGGGAAAACAGGCGTT
ACCGTGCAAAGCAGCCTCGATGCGGTAAAAGATGATTTTGATGTGTTTATCGATTTTACC
CGTCCGGAAGGTACGCTGAACCATCTCGCTTTTTGTCGCCAGCATGGCAAAGGGATGGTG
ATCGGCACTACGGGGTTTGACGAAGCCGGTAAACAAGCAATTCGTGACGCCGCTGCCGAT
ATTGCGATTGTCTTTGCTGCCAATTTTAGCGTTGGCGTTAACGTCATGCTTAAGCTGCTG
GAGAAAGCAGCCAAAGTGATGGGTGACTACACCGATATCGAAATTATTGAAGCACATCAT
AGACATAAAGTTGATGCGCCGTCAGGCACCGCACTGGCAATGGGAGAGGCGATCGCCCAC
GCCCTTGATAAAGATCTGAAAGATTGCGCGGTCTACAGTCGTGAAGGCCACACCGGTGAA
CGTGTGCCTGGCACCATTGGTTTTGCCACCGTGCGTGCAGGTGACATCGTTGGTGAACAT
ACCGCGATGTTTGCCGATATTGGCGAGCGTCTGGAGATCACCCATAAGGCGTCCAGCCGT
ATGACATTTGCTAACGGCGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGT
CTTTTTGATATGCGAGATGTACTTGATCTCAATAATTTGTAA

Protein Properties

Pfam Domain Function:

SATase_N (PF06426 )

Protein Residues:

273

Protein Molecular Weight:

29316

Protein Theoretical pI:

PDB File:

1T3D

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Serine acetyltransferase
MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSALSYMLANKLSSPIMPA
IAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPLLYLKGFHALQAYRIGHWL
WNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHATGIVVGETAVIENDVSILQSV
TLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGAKIGAGSVVLQPVPPHTTAAGVP
ARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI

References

External Links:

Resource	Link
Uniprot ID:	P0A9D4
Uniprot Name:	CYSE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321913
PDB ID:	1T3D
Ecogene ID:	EG10187
Ecocyc:	EG10187
ColiBase:	b3607
Kegg Gene:	b3607
EchoBASE ID:	EB0184
CCDB:	CYSE_ECOLI
BacMap:	16131478

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Denk, D., Bock, A. (1987). "L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant." J Gen Microbiol 133:515-525. Pubmed: 3309158
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Hindson, V. J., Moody, P. C., Rowe, A. J., Shaw, W. V. (2000). "Serine acetyltransferase from Escherichia coli is a dimer of trimers." J Biol Chem 275:461-466. Pubmed: 10617639
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
Tei, H., Murata, K., Kimura, A. (1990). "Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus." Biochem Biophys Res Commun 167:948-955. Pubmed: 2108679
Wigley, D. B., Derrick, J. P., Shaw, W. V. (1990). "The serine acetyltransferase from Escherichia coli. Over-expression, purification and preliminary crystallographic analysis." FEBS Lett 277:267-271. Pubmed: 2125278