Uracil phosphoribosyltransferase (P0A8F0)

Identification
Name:Uracil phosphoribosyltransferase
Synonyms:
  • UMP pyrophosphorylase
  • UPRTase
Gene Name:upp
Enzyme Class:
Biological Properties
General Function:Involved in nucleoside metabolic process
Specific Function:Catalyzes the conversion of uracil and 5-phospho-alpha- D-ribose 1-diphosphate (PRPP) to UMP and diphosphate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoribosyl pyrophosphate + 1.0Uracil ↔ 1.0Pyrophosphate + 1.0Uridine 5'-monophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uridine 5'-monophosphate + 1.0Pyrophosphate ↔ 1.0Uracil + 1.0Phosphoribosyl pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoribosyl pyrophosphate + 1.0Uracil ↔ 1.0Pyrophosphate + 1.0Uridine 5'-monophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Uridine 5'-monophosphate + 1.0Pyrophosphate → 1.0Uracil + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00280Phosphoribosyl pyrophosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00300UracilMetaboCard
ECMDB00288Uridine 5'-monophosphateMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
uracil phosphoribosyltransferase activity
Process
cellular aromatic compound metabolic process
cellular metabolic process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
pyrimidine base metabolic process
uracil metabolic process
uracil salvage
Gene Properties
Blattner:b2498
Gene OrientationCounterclockwise
Centisome Percentage:56.43
Left Sequence End2618268
Right Sequence End2618894
Gene Sequence:
>627 bp
ATGAAGATCGTGGAAGTCAAACACCCACTCGTCAAACACAAGCTGGGACTGATGCGTGAG
CAAGATATCAGCACCAAGCGCTTTCGCGAACTCGCTTCCGAAGTGGGTAGCCTGCTGACT
TACGAAGCGACCGCCGACCTCGAAACGGAAAAAGTAACTATCGAAGGCTGGAACGGCCCG
GTAGAAATCGACCAGATCAAAGGTAAGAAAATTACCGTTGTGCCAATTCTGCGTGCGGGT
CTTGGTATGATGGACGGTGTGCTGGAAAACGTTCCGAGCGCGCGCATCAGCGTTGTCGGT
ATGTACCGTAATGAAGAAACGCTGGAGCCGGTACCGTACTTCCAGAAACTGGTTTCTAAC
ATCGATGAGCGTATGGCGCTGATCGTTGACCCAATGCTGGCAACCGGTGGTTCCGTTATC
GCGACCATCGACCTGCTGAAAAAAGCGGGCTGCAGCAGCATCAAAGTTCTGGTGCTGGTA
GCTGCGCCAGAAGGTATCGCTGCGCTGGAAAAAGCGCACCCGGACGTCGAACTGTATACC
GCATCGATTGATCAGGGACTGAACGAGCACGGATACATTATTCCGGGCCTCGGCGATGCC
GGTGACAAAATCTTTGGTACGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:208
Protein Molecular Weight:22533
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uracil phosphoribosyltransferase
MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEATADLETEKVTIEGWNGP
VEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMYRNEETLEPVPYFQKLVSN
IDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLVAAPEGIAALEKAHPDVELYT
ASIDQGLNEHGYIIPGLGDAGDKIFGTK
References
External Links:
ResourceLink
Uniprot ID:P0A8F0
Uniprot Name:UPP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1805558
Ecogene ID:EG11332
Ecocyc:EG11332
ColiBase:b2498
Kegg Gene:b2498
EchoBASE ID:EB1308
CCDB:UPP_ECOLI
BacMap:90111448
General Reference:
  • Andersen, P. S., Smith, J. M., Mygind, B. (1992). "Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12." Eur J Biochem 204:51-56. Pubmed: 1371255
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jensen, K. F., Mygind, B. (1996). "Different oligomeric states are involved in the allosteric behavior of uracil phosphoribosyltransferase from Escherichia coli." Eur J Biochem 240:637-645. Pubmed: 8856065
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Lundegaard, C., Jensen, K. F. (1999). "Kinetic mechanism of uracil phosphoribosyltransferase from Escherichia coli and catalytic importance of the conserved proline in the PRPP binding site." Biochemistry 38:3327-3334. Pubmed: 10079076
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837