Holliday junction ATP-dependent DNA helicase ruvB (P0A812)

Identification
Name:Holliday junction ATP-dependent DNA helicase ruvB
Synonyms:Not Available
Gene Name:ruvB
Enzyme Class:
Biological Properties
General Function:Replication, recombination and repair
Specific Function:The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday junction migration by localized denaturation and reannealing
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
DNA binding
DNA helicase activity
four-way junction helicase activity
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA recombination
DNA repair
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1011 bp
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGAT
CGCGCCATTCGCCCCAAATTACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAG
ATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATGCCCTCGATCATTTGTTGATT
TTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATG
CTCACTAACCTTGAACCGCATGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCA
GTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACCAACTGGATATCATGATTGGT
GAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGT
CTGGAGTTTTATCAGGTGCCGGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATG
GGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTCGTCGCGCTCGCGGTACGCCG
CGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGT
TTCGATTATATGGACCGCAAATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCT
GTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAACGTGAAACCATTGAGGATGTG
CTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:336
Protein Molecular Weight:37173
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Holliday junction ATP-dependent DNA helicase ruvB
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLI
FGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSP
VVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIVQR
LEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDV
LEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPEMP
References
External Links:
ResourceLink
Uniprot ID:P0A812
Uniprot Name:RUVB_ECOLI
GenBank Gene ID:M21298
Genebank Protein ID:147783
CCDB:RUVB_ECOLI
General Reference:
  • Benson, F. E., Illing, G. T., Sharples, G. J., Lloyd, R. G. (1988). "Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes." Nucleic Acids Res 16:1541-1549. Pubmed: 3279394
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Shinagawa, H., Makino, K., Amemura, M., Kimura, S., Iwasaki, H., Nakata, A. (1988). "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination." J Bacteriol 170:4322-4329. Pubmed: 2842314
  • Tsaneva, I. R., Muller, B., West, S. C. (1993). "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro." Proc Natl Acad Sci U S A 90:1315-1319. Pubmed: 8433990
  • West, S. C. (1997). "Processing of recombination intermediates by the RuvABC proteins." Annu Rev Genet 31:213-244. Pubmed: 9442895