Holliday junction ATP-dependent DNA helicase ruvA (P0A809)

Identification
Name:Holliday junction ATP-dependent DNA helicase ruvA
Synonyms:Not Available
Gene Name:ruvA
Enzyme Class:
Biological Properties
General Function:Replication, recombination and repair
Specific Function:The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of ruvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
DNA helicase complex
Holliday junction helicase complex
macromolecular complex
protein complex
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
DNA binding
DNA helicase activity
four-way junction helicase activity
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside binding
nucleoside-triphosphatase activity
purine nucleoside binding
pyrophosphatase activity
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA recombination
DNA repair
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>612 bp
GTGATAGGCAGACTCAGAGGCATCATCATTGAAAAACAACCCCCGCTGGTGTTAATTGAA
GTGGGCGGCGTAGGCTATGAAGTGCATATGCCGATGACCTGTTTTTATGAACTCCCTGAA
GCGGGTCAGGAAGCGATCGTTTTCACCCACTTTGTGGTGCGTGAAGACGCGCAACTGCTG
TACGGTTTTAACAATAAACAAGAGCGCACATTGTTCAAAGAGTTGATCAAAACCAACGGC
GTCGGCCCGAAGTTGGCGCTGGCGATCCTCTCCGGAATGTCAGCGCAGCAGTTCGTTAAT
GCCGTTGAGCGTGAAGAAGTGGGGGCACTGGTGAAACTGCCGGGTATTGGCAAAAAAACC
GCCGAACGCTTGATTGTTGAAATGAAAGACCGATTTAAAGGTTTGCATGGCGATCTCTTT
ACGCCAGCCGCCGACCTGGTACTCACGTCTCCTGCCAGCCCGGCGACCGACGATGCTGAA
CAAGAAGCGGTTGCCGCGCTGGTGGCGCTGGGCTATAAACCACAAGAAGCAAGCCGCATG
GTGAGCAAAATCGCTCGCCCTGACGCCAGCAGTGAAACTTTAATTCGCGAAGCCCTACGC
GCCGCGTTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:203
Protein Molecular Weight:22086
Protein Theoretical pI:6
PDB File:1C7Y
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Holliday junction ATP-dependent DNA helicase ruvA
MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLL
YGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKT
AERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRM
VSKIARPDASSETLIREALRAAL
References
External Links:
ResourceLink
Uniprot ID:P0A809
Uniprot Name:RUVA_ECOLI
GenBank Gene ID:M21298
Genebank Protein ID:147782
PDB ID:1C7Y
CCDB:RUVA_ECOLI
General Reference:
  • Benson, F. E., Illing, G. T., Sharples, G. J., Lloyd, R. G. (1988). "Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes." Nucleic Acids Res 16:1541-1549. Pubmed: 3279394
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hargreaves, D., Rice, D. W., Sedelnikova, S. E., Artymiuk, P. J., Lloyd, R. G., Rafferty, J. B. (1998). "Crystal structure of E.coli RuvA with bound DNA Holliday junction at 6 A resolution." Nat Struct Biol 5:441-446. Pubmed: 9628481
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Nishino, T., Ariyoshi, M., Iwasaki, H., Shinagawa, H., Morikawa, K. (1998). "Functional analyses of the domain structure in the Holliday junction binding protein RuvA." Structure 6:11-21. Pubmed: 9493263
  • Nishino, T., Iwasaki, H., Kataoka, M., Ariyoshi, M., Fujita, T., Shinagawa, H., Morikawa, K. (2000). "Modulation of RuvB function by the mobile domain III of the Holliday junction recognition protein RuvA." J Mol Biol 298:407-416. Pubmed: 10772859
  • Rafferty, J. B., Sedelnikova, S. E., Hargreaves, D., Artymiuk, P. J., Baker, P. J., Sharples, G. J., Mahdi, A. A., Lloyd, R. G., Rice, D. W. (1996). "Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction." Science 274:415-421. Pubmed: 8832889
  • Shinagawa, H., Makino, K., Amemura, M., Kimura, S., Iwasaki, H., Nakata, A. (1988). "Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination." J Bacteriol 170:4322-4329. Pubmed: 2842314
  • Takahagi, M., Iwasaki, H., Nakata, A., Shinagawa, H. (1991). "Molecular analysis of the Escherichia coli ruvC gene, which encodes a Holliday junction-specific endonuclease." J Bacteriol 173:5747-5753. Pubmed: 1885548
  • Tsaneva, I. R., Muller, B., West, S. C. (1993). "RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro." Proc Natl Acad Sci U S A 90:1315-1319. Pubmed: 8433990
  • West, S. C. (1997). "Processing of recombination intermediates by the RuvABC proteins." Annu Rev Genet 31:213-244. Pubmed: 9442895