Identification
Name:Inorganic pyrophosphatase
Synonyms:
  • Pyrophosphate phospho-hydrolase
  • PPase
Gene Name:ppa
Enzyme Class:
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:Diphosphate + H(2)O = 2 phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+2.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb2.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
inorganic diphosphatase activity
ion binding
magnesium ion binding
metal ion binding
pyrophosphatase activity
Process
cellular metabolic process
metabolic process
phosphate metabolic process
phosphorus metabolic process
Gene Properties
Blattner:b4226
Gene OrientationCounterclockwise
Centisome Percentage:95.85
Left Sequence End4447145
Right Sequence End4447675
Gene Sequence:
>531 bp
ATGATTCTTATAATTTATGCGCATCCGTATCCGCATCATTCCCATGCGAATAAACGGATG
CTTGAACAGGCAAGGACGCTGGAAGGCGTCGAAATTCGCTCTCTTTATCAACTCTATCCT
GACTTCAATATCGATATTGCCGCCGAGCAGGAGGCGCTGTCTCGCGCCGATCTGATCGTC
TGGCAGCATCCGATGCAGTGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAA
GTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTG
CTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGC
TTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTG
CCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCG
CGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAG
Protein Properties
Pfam Domain Function:
Protein Residues:176
Protein Molecular Weight:19703
Protein Theoretical pI:5
PDB File:2EIP
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Inorganic pyrophosphatase
MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDRFMSTAMFYPCNYGYIN
HTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGEDAKLVAVPHSKLSKEYDH
IKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAEAAKAEIVASFERAKNK
References
External Links:
ResourceLink
Uniprot ID:P0A7A9
Uniprot Name:IPYR_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674301
PDB ID:2EIP
Ecogene ID:EG10755
Ecocyc:EG10755
ColiBase:b4226
Kegg Gene:b4226
EchoBASE ID:EB0748
CCDB:IPYR_ECOLI
BacMap:16132048
General Reference:
  • Avaeva, S., Kurilova, S., Nazarova, T., Rodina, E., Vorobyeva, N., Sklyankina, V., Grigorjeva, O., Harutyunyan, E., Oganessyan, V., Wilson, K., Dauter, Z., Huber, R., Mather, T. (1997). "Crystal structure of Escherichia coli inorganic pyrophosphatase complexed with SO4(2-). Ligand-induced molecular asymmetry." FEBS Lett 410:502-508. Pubmed: 9237692
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Harutyunyan, E. H., Oganessyan, V. Y., Oganessyan, N. N., Avaeva, S. M., Nazarova, T. I., Vorobyeva, N. N., Kurilova, S. A., Huber, R., Mather, T. (1997). "Crystal structure of holo inorganic pyrophosphatase from Escherichia coli at 1.9 A resolution. Mechanism of hydrolysis." Biochemistry 36:7754-7760. Pubmed: 9201917
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kaneko, S., Ichiba, T., Hirano, N., Hachimori, A. (1993). "Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli." Int J Biochem 25:233-238. Pubmed: 8383066
  • Kankare, J., Neal, G. S., Salminen, T., Glumoff, T., Glumhoff T [corrected to Glumoff, T., Cooperman, B. S., Lahti, R., Goldman, A. (1994). "The structure of E.coli soluble inorganic pyrophosphatase at 2.7 A resolution." Protein Eng 7:823-830. Pubmed: 7971944
  • Kankare, J., Salminen, T., Lahti, R., Cooperman, B. S., Baykov, A. A., Goldman, A. (1996). "Crystallographic identification of metal-binding sites in Escherichia coli inorganic pyrophosphatase." Biochemistry 35:4670-4677. Pubmed: 8664256
  • Kankare, J., Salminen, T., Lahti, R., Cooperman, B. S., Baykov, A. A., Goldman, A. (1996). "Structure of Escherichia coli inorganic pyrophosphatase at 2.2 A resolution." Acta Crystallogr D Biol Crystallogr 52:551-563. Pubmed: 15299678
  • Lahti, R., Kolakowski, L. F. Jr, Heinonen, J., Vihinen, M., Pohjanoksa, K., Cooperman, B. S. (1990). "Conservation of functional residues between yeast and E. coli inorganic pyrophosphatases." Biochim Biophys Acta 1038:338-345. Pubmed: 2160278
  • Lahti, R., Pitkaranta, T., Valve, E., Ilta, I., Kukko-Kalske, E., Heinonen, J. (1988). "Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12." J Bacteriol 170:5901-5907. Pubmed: 2848015
  • Lahti, R., Pohjanoksa, K., Pitkaranta, T., Heikinheimo, P., Salminen, T., Meyer, P., Heinonen, J. (1990). "A site-directed mutagenesis study on Escherichia coli inorganic pyrophosphatase. Glutamic acid-98 and lysine-104 are important for structural integrity, whereas aspartic acids-97 and -102 are essential for catalytic activity." Biochemistry 29:5761-5766. Pubmed: 1974462
  • Lahti, R., Salminen, T., Latonen, S., Heikinheimo, P., Pohjanoksa, K., Heinonen, J. (1991). "Genetic engineering of Escherichia coli inorganic pyrophosphatase. Tyr55 and Tyr141 are important for the structural integrity." Eur J Biochem 198:293-297. Pubmed: 1645654
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646