Thymidylate kinase (P0A720)

Identification
Name:Thymidylate kinase
Synonyms:
  • Thymidine monophosphate kinase
  • dTMP kinase
  • TMPK
Gene Name:tmk
Enzyme Class:
Biological Properties
General Function:Involved in thymidylate kinase activity
Specific Function:Catalyzes the reversible phosphorylation of deoxythymidine monophosphate (dTMP) to deoxythymidine diphosphate (dTDP), using ATP as its preferred phosphoryl donor. Situated at the junction of both de novo and salvage pathways of deoxythymidine triphosphate (dTTP) synthesis, is essential for DNA synthesis and cellular growth
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.05-Thymidylic acid ↔ 1.0ADP + 1.0dTDP
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0dUDP
1.0Adenosine triphosphate + 1.0dUMP ↔ 1.0ADP + 1.0dUDP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.05-Thymidylic acid ↔ 1.0ADP + 1.0dTDP
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.05-Thymidylic acid + 1.0Adenosine triphosphate → 1.0dTDP + 1.0ADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB012275-Thymidylic acidMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01274dTDPMetaboCard
ECMDB01000dUDPMetaboCard
ECMDB01409dUMPMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
nucleoside binding
phosphotransferase activity, phosphate group as acceptor
purine nucleoside binding
thymidylate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular nitrogen compound metabolic process
dTDP biosynthetic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
pyrimidine deoxyribonucleoside diphosphate biosynthetic process
pyrimidine nucleoside diphosphate biosynthetic process
pyrimidine nucleotide biosynthetic process
pyrimidine nucleotide metabolic process
Gene Properties
Blattner:b1098
Gene OrientationClockwise
Centisome Percentage:24.88
Left Sequence End1154347
Right Sequence End1154988
Gene Sequence:
>642 bp
ATGCGCAGTAAGTATATCGTCATTGAGGGGCTGGAAGGCGCAGGCAAAACTACCGCGCGT
AATGTGGTGGTTGAGACGCTCGAGCAACTGGGTATCCGCGACATGGTTTTCACTCGGGAA
CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTA
GGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAA
CTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGC
CACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTG
GCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGAT
GTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAG
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAA
GATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATC
CGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:213
Protein Molecular Weight:23783
Protein Theoretical pI:5
PDB File:5TMP
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thymidylate kinase
MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGTQLAEKLRSLVLDIKSV
GDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHDLSTQAYQGGGRGIDQHML
ATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIEQESFDFFNRTRARYLELAAQ
DKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA
References
External Links:
ResourceLink
Uniprot ID:P0A720
Uniprot Name:KTHY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062665
PDB ID:5TMP
Ecogene ID:EG12302
Ecocyc:EG12302
ColiBase:b1098
Kegg Gene:b1098
EchoBASE ID:EB2208
CCDB:KTHY_ECOLI
BacMap:16129061
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, J. R., Franden, M. A., Aebersold, R., McHenry, C. S. (1993). "Identification, isolation, and characterization of the structural gene encoding the delta' subunit of Escherichia coli DNA polymerase III holoenzyme." J Bacteriol 175:3812-3822. Pubmed: 8509334
  • Dong, Z., Onrust, R., Skangalis, M., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'." J Biol Chem 268:11758-11765. Pubmed: 8505303
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lavie, A., Ostermann, N., Brundiers, R., Goody, R. S., Reinstein, J., Konrad, M., Schlichting, I. (1998). "Structural basis for efficient phosphorylation of 3'-azidothymidine monophosphate by Escherichia coli thymidylate kinase." Proc Natl Acad Sci U S A 95:14045-14050. Pubmed: 9826650
  • Munier-Lehmann, H., Chaffotte, A., Pochet, S., Labesse, G. (2001). "Thymidylate kinase of Mycobacterium tuberculosis: a chimera sharing properties common to eukaryotic and bacterial enzymes." Protein Sci 10:1195-1205. Pubmed: 11369858
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Reynes, J. P., Tiraby, M., Baron, M., Drocourt, D., Tiraby, G. (1996). "Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus." J Bacteriol 178:2804-2812. Pubmed: 8631667