Ribose-phosphate pyrophosphokinase (P0A717)

Identification
Name:Ribose-phosphate pyrophosphokinase
Synonyms:
  • RPPK
  • Phosphoribosyl pyrophosphate synthase
  • P-Rib-PP synthase
  • PRPP synthase
Gene Name:prs
Enzyme Class:
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:ATP + D-ribose 5-phosphate = AMP + 5-phospho- alpha-D-ribose 1-diphosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Ribose-5-phosphate ↔ 1.0Adenosine monophosphate + 1.0Hydrogen ion + 1.0Phosphoribosyl pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Ribose-5-phosphate ↔ 1.0Adenosine monophosphate + 1.0Phosphoribosyl pyrophosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Ribose-5-phosphate + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Adenosine monophosphate + 1.0Phosphoribosyl pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Ribose-5-phosphate ↔ 1.0Adenosine monophosphate + 1.0Hydrogen ion + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0D-Ribose-5-phosphate → 1.0Adenosine monophosphate + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB02033D-Ribose-5-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00280Phosphoribosyl pyrophosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
diphosphotransferase activity
ion binding
magnesium ion binding
metal ion binding
ribose phosphate diphosphokinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular biosynthetic process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
nucleoside monophosphate biosynthetic process
nucleoside monophosphate metabolic process
nucleoside phosphate metabolic process
nucleotide biosynthetic process
nucleotide metabolic process
ribonucleoside monophosphate biosynthetic process
Gene Properties
Blattner:b1207
Gene OrientationCounterclockwise
Centisome Percentage:27.16
Left Sequence End1260151
Right Sequence End1261098
Gene Sequence:
>948 bp
GTGCCTGATATGAAGCTTTTTGCTGGTAACGCCACCCCGGAACTAGCACAACGTATTGCC
AACCGCCTGTACACTTCACTCGGCGACGCCGCTGTAGGTCGCTTTAGCGATGGCGAAGTC
AGCGTACAAATTAATGAAAATGTACGCGGTGGTGATATTTTCATCATCCAGTCCACTTGT
GCCCCTACTAACGACAACCTGATGGAATTAGTCGTTATGGTTGATGCCCTGCGTCGTGCT
TCCGCAGGTCGTATCACCGCTGTTATCCCCTACTTTGGCTATGCGCGCCAGGACCGTCGC
GTCCGTTCCGCTCGTGTACCAATCACTGCGAAAGTGGTTGCAGACTTCCTCTCCAGCGTC
GGTGTTGACCGTGTGCTGACAGTGGATCTGCACGCTGAACAGATTCAGGGTTTCTTCGAC
GTTCCGGTTGATAACGTATTTGGTAGCCCGATCCTGCTGGAAGACATGCTGCAGCTGAAT
CTGGATAACCCAATTGTGGTTTCTCCGGACATCGGCGGCGTTGTGCGTGCCCGCGCTATC
GCTAAGCTGCTGAACGATACCGATATGGCAATCATCGACAAACGTCGTCCGCGTGCGAAC
GTTTCACAGGTGATGCATATCATCGGTGACGTTGCAGGTCGTGACTGCGTACTGGTCGAT
GATATGATCGACACTGGCGGTACGCTGTGTAAAGCTGCTGAAGCTCTGAAAGAACGTGGT
GCTAAACGTGTATTTGCGTACGCGACTCACCCGATCTTCTCTGGCAACGCGGCGAACAAC
CTGCGTAACTCTGTAATTGATGAAGTCGTTGTCTGCGATACCATTCCGCTGAGCGATGAA
ATCAAATCACTGCCGAACGTGCGTACTCTGACCCTGTCAGGTATGCTGGCCGAAGCGATT
CGTCGTATCAGCAACGAAGAATCGATCTCTGCCATGTTCGAACACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:315
Protein Molecular Weight:34218
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribose-phosphate pyrophosphokinase
MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTC
APTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSV
GVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLNLDNPIVVSPDIGGVVRARAI
AKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERG
AKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLSDEIKSLPNVRTLTLSGMLAEAI
RRISNEESISAMFEH
References
External Links:
ResourceLink
Uniprot ID:P0A717
Uniprot Name:KPRS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062790
Ecogene ID:EG10774
Ecocyc:EG10774
ColiBase:b1207
Kegg Gene:b1207
EchoBASE ID:EB0767
CCDB:KPRS_ECOLI
BacMap:16129170
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bower, S. G., Harlow, K. W., Switzer, R. L., Hove-Jensen, B. (1989). "Characterization of the Escherichia coli prsA1-encoded mutant phosphoribosylpyrophosphate synthetase identifies a divalent cation-nucleotide binding site." J Biol Chem 264:10287-10291. Pubmed: 2542328
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hove-Jensen, B., Harlow, K. W., King, C. J., Switzer, R. L. (1986). "Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene." J Biol Chem 261:6765-6771. Pubmed: 3009477
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232