ECMDB

Identification

Name:

Glutaminase 2

Synonyms:

Not Available

Gene Name:

glsA2

Enzyme Class:

3.5.1.2

Biological Properties

General Function:

Involved in glutaminase activity

Specific Function:

L-glutamine + H(2)O = L-glutamate + NH(3)

Cellular Location:

Cytoplasmic

SMPDB Pathways:

D-Glutamine and D-glutamate metabolism PW000769
L-glutamate metabolism PW000789
L-glutamate metabolism II PW001886
Nitrogen metabolism PW000755

KEGG Pathways:

Alanine, aspartate and glutamate metabolism ec00250
Arginine and proline metabolism ec00330
D-Glutamine and D-glutamate metabolism ec00471
Metabolic pathways eco01100
Nitrogen metabolism ec00910

KEGG Reactions:

1.0

↔

1.0

1.0L-Glutamine + 1.0Water ↔ 1.0L-Glutamate + 1.0Ammonia
ReactionCard

1.0

↔

1.0

1.0D-Glutamine + 1.0Water ↔ 1.0D-Glutamic acid + 1.0Ammonia
ReactionCard

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonia
ReactionCard

SMPDB Reactions:

1.0

→

1.0L-Glutamic acid

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamic acid + 1.0Ammonia + 1.0L-Glutamate
ReactionCard

1.0

→

1.0

1.0D-Glutamine + 1.0Water → 1.0D-Glutamic acid + 1.0Ammonia
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0L-Glutamine + 1.0Water ↔ 1.0L-Glutamate + 1.0Ammonia
ReactionCard

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonia
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0L-Glutamine + 1.0Water → 1.0L-Glutamate + 1.0Ammonium
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB03339	D-Glutamic acid	MetaboCard
ECMDB03423	D-Glutamine	MetaboCard
ECMDB00148	L-Glutamate	MetaboCard
ECMDB00641	L-Glutamine	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
glutaminase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
glutamine metabolic process
metabolic process

Gene Properties

Blattner:

b1524

Gene Orientation

Counterclockwise

Centisome Percentage:

34.71

Left Sequence End

1610349

Right Sequence End

1611275

Gene Sequence:

>927 bp
ATGACCATTGCACTGGAACTTCAACAGCTTAAAAAAACCTATCCAGGCGGCGTTCAGGCG
CTTCGTGGGATAGATTTGCAGGTCGAAGCGGGTGATTTTTATGCGCTTCTCGGGCCGAAC
GGGGCCGGGAAATCGACCACTATCGGTATTATCAGCTCTCTGGTAAATAAAACCTCCGGG
CGGGTCAGCGTATTTGGTTACGATCTCGAGAAGGATGTCGTGAACGCTAAACGTCAGTTG
GGACTGGTGCCGCAGGAATTTAACTTCAACCCGTTTGAAACCGTGCAGCAAATTGTGGTG
AATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTAT
CTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGG
ATGAAGCGCCGTTTAATGATTGCCCGTGCGTTAATGCATGAACCTAAACTACTGATTCTC
GACGAACCGACCGCAGGCGTGGATATTGAACTTCGCCGCTCAATGTGGGGCTTTTTGAAG
GATTTAAACGACAAAGGCACCACCATCATTCTCACCACACACTACCTGGAAGAAGCAGAA
ATGCTGTGCCGCAATATCGGCATTATTCAACACGGTGAGCTGGTGGAAAATACCTCGATG
AAGGCGCTGCTGGCGAAGCTGAAATCGGAAACCTTTATTCTCGATCTCGCACCGAAAAGC
CCGTTACCGAAGCTCGATGGCTATCAGTATCGACTGGTCGATACCGCGACGCTGGAAGTT
GAAGTGCTGCGTGAGCAGGGGATCAACAGCGTATTTACGCAGTTAAGTGAGCAGGGCATT
CAGGTATTAAGTATGCGTAACAAAGCTAACCGTCTGGAAGAGCTGTTTGTTTCACTGGTT
AATGAAAAACAAGGAGATCGCGCATGA

Protein Properties

Pfam Domain Function:

Glutaminase (PF04960 )

Protein Residues:

308

Protein Molecular Weight:

33515

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Glutaminase 2
MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIAICTVDGQLFQAGDAQE
RFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLVQLEMEQGIPRNPFINAGA
LVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARSEFEHSARNAAIAWLMKSFGN
FHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKAIHIDEPVVTPMQARQINALMAT
SGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAIAVWSPELDDAGNSLAGIAVLEQLT
KQLGRSVY

References

External Links:

Resource	Link
Uniprot ID:	P0A6W0
Uniprot Name:	GLSA2_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21238982
Ecogene ID:	EG13816
Ecocyc:	EG13816
ColiBase:	b1524
Kegg Gene:	b1524
EchoBASE ID:	EB3577
CCDB:	GLSA2_ECOLI
BacMap:	16129483

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

Glutaminase 2 (P0A6W0)