Identification
Name:GTP cyclohydrolase 1
Synonyms:
  • GTP cyclohydrolase I
  • GTP-CH-I
Gene Name:folE
Enzyme Class:
Biological Properties
General Function:Involved in GTP cyclohydrolase I activity
Specific Function:GTP + H(2)O = formate + 2-amino-4-hydroxy-6- (erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+3.0Thumb1.0Thumb+1.0Pyrophosphate+2.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Pyrophosphate
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.02-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
1.0Guanosine triphosphate + 1.0Water → 1.0Formic acid + 1.02-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200582,5-Diamino-6-(5'-phosphoribosylamino)-4-pyrimidineoneMetaboCard
ECMDB068232,5-Diamino-6-(5'-triphosphoryl-3',4'-trihydroxy-2'-oxopentyl)-amino-4-oxopyrimidineMetaboCard
ECMDB040522,5-Diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidineMetaboCard
ECMDB200592,5-Diaminopyrimidine nucleoside triphosphateMetaboCard
ECMDB242457,8-dihydroneopterin 3'-triphosphateMetaboCard
ECMDB01144Dihydroneopterin triphosphateMetaboCard
ECMDB20148Formamidopyrimidine nucleoside triphosphateMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
cyclohydrolase activity
GTP cyclohydrolase activity
GTP cyclohydrolase I activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
Process
cellular aromatic compound metabolic process
cellular metabolic process
folic acid and derivative biosynthetic process
folic acid and derivative metabolic process
metabolic process
tetrahydrofolate biosynthetic process
Gene Properties
Blattner:b2153
Gene OrientationCounterclockwise
Centisome Percentage:48.30
Left Sequence End2241006
Right Sequence End2241674
Gene Sequence:
>669 bp
ATGCCCGTCAAGGATTTGACGGGCATTACTGCAAAGGACGCGCAAATGTTATCTGTAGTT
AAACCTCTTCAGGAATTTGGTAAGCTCGATAAATGTTTGTCCAGATACGGTACGCGCTTC
GAGTTTAATAATGAAAAGCAAGTTATATTTTCCAGTGATGTCAATAACGAAGATACTTTC
GTTATTTTAGAGGGAGTTATCTCTCTGCGTAGAGAAGAAAACGTACTTATCGGTATTACC
CAGGCTCCTTATATTATGGGGCTGGCTGATGGTTTAATGAAAAACGATATACCATACAAA
TTAATATCAGAAGGAAATTGTACGGGATATCATCTACCAGCCAAACAAACCATTACGCTT
ATTGAACAAAATCAACTCTGGCGAGACGCTTTTTACTGGTTAGCCTGGCAAAATAGAATT
CTGGAATTACGCGACGTGCAGCTCATTGGGCATAATTCCTACGAACAAATCCGCGCAACA
TTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTAT
ATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGT
AAAGGCGGCTATATCGAAATGAATAAAGGCAAACTGGTCGCTATCAACCGTTTGCCTTCA
GAGTATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:222
Protein Molecular Weight:24830
Protein Theoretical pI:7
PDB File:1GTP
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GTP cyclohydrolase 1
MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHMTEIMQLLNLDLADDSL
METPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTVRDITLTSTCEHHFVTIDG
KATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQILIALQTLLGTNNVAVSIDAVH
YCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAVRHHN
References
External Links:
ResourceLink
Uniprot ID:P0A6T5
Uniprot Name:GCH1_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674516
PDB ID:1GTP
Ecogene ID:EG11375
Ecocyc:EG11375
ColiBase:b2153
Kegg Gene:b2153
EchoBASE ID:EB1349
CCDB:GCH1_ECOLI
BacMap:16130091
General Reference:
  • Auerbach, G., Herrmann, A., Bracher, A., Bader, G., Gutlich, M., Fischer, M., Neukamm, M., Garrido-Franco, M., Richardson, J., Nar, H., Huber, R., Bacher, A. (2000). "Zinc plays a key role in human and bacterial GTP cyclohydrolase I." Proc Natl Acad Sci U S A 97:13567-13572. Pubmed: 11087827
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Katzenmeier, G., Schmid, C., Kellermann, J., Lottspeich, F., Bacher, A. (1991). "Biosynthesis of tetrahydrofolate. Sequence of GTP cyclohydrolase I from Escherichia coli." Biol Chem Hoppe Seyler 372:991-997. Pubmed: 1665332
  • Lee, S., Ahn, C., Park, E., Hwang, D. S., Yim, J. (2002). "Biochemical characterization of oligomerization of Escherichia coli GTP cyclohydrolase I." J Biochem Mol Biol 35:255-261. Pubmed: 12297008
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Nar, H., Huber, R., Meining, W., Schmid, C., Weinkauf, S., Bacher, A. (1995). "Atomic structure of GTP cyclohydrolase I." Structure 3:459-466. Pubmed: 7663943
  • Rebelo, J., Auerbach, G., Bader, G., Bracher, A., Nar, H., Hosl, C., Schramek, N., Kaiser, J., Bacher, A., Huber, R., Fischer, M. (2003). "Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I." J Mol Biol 326:503-516. Pubmed: 12559918
  • Schmid, C., Meining, W., Weinkauf, S., Bachmann, L., Ritz, H., Eberhardt, S., Gimbel, W., Werner, T., Lahm, H. W., Nar, H., et, a. l. .. (1993). "Studies on GTP cyclohydrolase I of Escherichia coli." Adv Exp Med Biol 338:157-162. Pubmed: 8304099
  • Schoedon, G., Redweik, U., Frank, G., Cotton, R. G., Blau, N. (1992). "Allosteric characteristics of GTP cyclohydrolase I from Escherichia coli." Eur J Biochem 210:561-568. Pubmed: 1459137