3-phosphoshikimate 1-carboxyvinyltransferase (P0A6D3)

Identification
Name:3-phosphoshikimate 1-carboxyvinyltransferase
Synonyms:
  • 5-enolpyruvylshikimate-3-phosphate synthase
  • EPSP synthase
  • EPSPS
Gene Name:aroA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring alkyl or aryl (other than methyl) groups
Specific Function:Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Shikimate 3-phosphate ↔ 1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0shikimate 3-phosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0shikimate 3-phosphate + 1.0Phosphoenolpyruvic acid + 1.0Shikimate 3-phosphate → 1.0Phosphate + 1.05-enolpyruvyl-shikimate 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Shikimate 3-phosphate ↔ 1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Shikimate 3-phosphate → 1.0Inorganic phosphate + 1.05-O-(1-Carboxyvinyl)-3-phosphoshikimate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB242035-enolpyruvyl-shikimate 3-phosphateMetaboCard
ECMDB201065-O-(1-Carboxyvinyl)-3-phosphoshikimateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB20193Shikimate 3-phosphateMetaboCard
GO Classification:
Function
3-phosphoshikimate 1-carboxyvinyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Gene Properties
Blattner:b0908
Gene OrientationClockwise
Centisome Percentage:20.65
Left Sequence End958035
Right Sequence End959318
Gene Sequence:
>1284 bp
GTGTCGAATAACGCTTTACAAACAATTATTAACGCCCGGTTACCAGGCGAAGAGGGGCTG
TGGCAGATTCATCTGCAGGACGGAAAAATCAGCGCCATTGATGCGCAATCCGGCGTGATG
CCCATAACTGAAAACAGCCTGGATGCCGAACAAGGTTTAGTTATACCGCCGTTTGTGGAG
CCACATATTCACCTGGACACCACGCAAACCGCCGGACAACCGAACTGGAATCAGTCCGGC
ACGCTGTTTGAAGGCATTGAACGCTGGGCCGAGCGCAAAGCGTTATTAACCCATGACGAT
GTGAAACAACGCGCATGGCAAACGCTGAAATGGCAGATTGCCAACGGCATTCAGCATGTG
CGTACCCATGTCGATGTTTCGGATGCAACGCTAACTGCGCTGAAAGCAATGCTGGAAGTG
AAGCAGGAAGTCGCGCCGTGGATTGATCTGCAAATCGTCGCCTTCCCTCAGGAAGGGATT
TTGTCGTATCCCAACGGTGAAGCGTTGCTGGAAGAGGCGTTACGCTTAGGGGCAGATGTA
GTGGGGGCGATTCCGCATTTTGAATTTACCCGTGAATACGGCGTGGAGTCGCTGCATAAA
ACCTTCGCCCTGGCGCAAAAATACGACCGTCTCATCGACGTTCACTGTGATGAGATCGAT
GACGAGCAGTCGCGCTTTGTCGAAACCGTTGCTGCCCTGGCGCACCATGAAGGCATGGGC
GCGCGAGTCACCGCCAGCCACACCACGGCAATGCACTCCTATAACGGGGCGTATACCTCA
CGCCTGTTCCGCTTGCTGAAAATGTCCGGTATTAACTTTGTCGCCAACCCGCTGGTCAAT
ATTCATCTGCAAGGACGTTTCGATACGTATCCAAAACGTCGCGGCATCACGCGCGTTAAA
GAGATGCTGGAGTCCGGCATTAACGTCTGCTTTGGTCACGATGATGTCTTCGATCCGTGG
TATCCGCTGGGAACGGCGAATATGCTGCAAGTGCTGCATATGGGGCTGCATGTTTGCCAG
TTGATGGGCTACGGGCAGATTAACGATGGCCTGAATTTAATCACCCACCACAGCGCAAGG
ACGTTGAATTTGCAGGATTACGGCATTGCCGCCGGAAACAGCGCCAACCTGATTATCCTG
CCGGCTGAAAATGGGTTTGATGCGCTGCGCCGTCAGGTTCCGGTACGTTATTCGGTACGT
GGCGGCAAGGTGATTGCCAGCACACAACCGGCACAAACCACCGTATATCTGGAGCAGCCA
GAAGCCATCGATTACAAACGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:427
Protein Molecular Weight:46095
Protein Theoretical pI:5
PDB File:1X8T
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-phosphoshikimate 1-carboxyvinyltransferase
MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNLLDSDDVRHMLNALTAL
GVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPLAAALCLGSNDIVLTGEPR
MKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGGNVDVDGSVSSQFLTALLMTA
PLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQHYQQFVVKGGQSYQSPGTYLVE
GDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRFADVLEKMGATICWGDDYISCTRGE
LNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNIYNWRVKETDRLFAMATELRKVGAEVE
EGHDYIRITPPEKLNFAEIATYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL
ARISQAA
References
External Links:
ResourceLink
Uniprot ID:P0A6D3
Uniprot Name:AROA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674479
PDB ID:1X8T
Ecogene ID:EG10073
Ecocyc:EG10073
ColiBase:b0908
Kegg Gene:b0908
EchoBASE ID:EB0071
CCDB:AROA_ECOLI
BacMap:16128875
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Huynh, Q. K. (1991). "5-Enolpyruvylshikimate-3-phosphate synthase from Escherichia coli--the substrate analogue bromopyruvate inactivates the enzyme by modifying Cys-408 and Lys-411." Arch Biochem Biophys 284:407-412. Pubmed: 1899181
  • Huynh, Q. K. (1993). "Photo-oxidation of 5-enolpyruvoylshikimate-3-phosphate synthase from Escherichia coli: evidence for a reactive imidazole group (His385) at the herbicide glyphosate-binding site." Biochem J 290 ( Pt 2):525-530. Pubmed: 8452542
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Stallings, W. C., Abdel-Meguid, S. S., Lim, L. W., Shieh, H. S., Dayringer, H. E., Leimgruber, N. K., Stegeman, R. A., Anderson, K. S., Sikorski, J. A., Padgette, S. R., Kishore, G. M. (1991). "Structure and topological symmetry of the glyphosate target 5-enolpyruvylshikimate-3-phosphate synthase: a distinctive protein fold." Proc Natl Acad Sci U S A 88:5046-5050. Pubmed: 11607190