PTS system beta-glucoside-specific EIIBCA component (P08722)

Identification
Name:PTS system beta-glucoside-specific EIIBCA component
Synonyms:
  • EIIBCA-Bgl
  • EII-Bgl
  • Beta-glucoside-specific phosphotransferase enzyme IIB component
  • PTS system beta-glucoside-specific EIIB component
  • Beta-glucoside permease IIC component
  • PTS system beta-glucoside-specific EIIC component
  • Beta-glucoside-specific phosphotransferase enzyme IIA component
  • PTS system beta-glucoside-specific EIIA component
Gene Name:bglF
Enzyme Class:
Biological Properties
General Function:Involved in protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
Specific Function:Acts as both a kinase and a phosphatase on BglG
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB → 1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine+1.0Thumb → 1.0protein EIIB+1.0sugar phosphate
1.0Protein EIIB N(pi)-phospho-L-histidine/cysteine + 1.0Sucrose → 1.0protein EIIB + 1.0sugar phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20117Arbutin 6-phosphateMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB20267Cellobiose-6-phosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB04151SalicinMetaboCard
ECMDB00258SucroseMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
carbohydrate transmembrane transporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b3722
Gene OrientationCounterclockwise
Centisome Percentage:84.10
Left Sequence End3901743
Right Sequence End3903620
Gene Sequence:
>1878 bp
ATGACGGAGTTAGCCAGAAAAATAGTCGCAGGAGTCGGGGGCGCAGATAACATTGTGAGT
CTGATGCATTGCGCAACGCGATTACGTTTTAAATTAAAGGATGAAAGCAAAGCGCAAGCA
GAGGTACTGAAAAAGACCCCCGGTATTATTATGGTGGTGGAAAGCGGTGGCCAGTTTCAG
GTGGTCATAGGTAACCATGTGGCCGATGTCTTCCTGGCGGTTAACAGTGTGGCAGGCCTT
GACGAAAAAGCGCAACAGGCACCGGAAAATGATGATAAAGGTAATCTGCTAAACCGCTTT
GTTTATGTTATTTCAGGTATTTTTACGCCTCTGATCGGTTTGATGGCGGCAACCGGGATC
TTGAAAGGTATGCTGGCTCTGGCGCTCACTTTTCAGTGGACGACCGAACAAAGTGGTACT
TATTTAATTTTATTCAGCGCCAGTGATGCCTTGTTTTGGTTCTTCCCGATAATCCTGGGA
TACACCGCGGGGAAACGCTTCGGCGGTAATCCATTTACTGCCATGGTGATTGGTGGAGCG
TTAGTGCATCCATTAATTCTGACTGCTTTCGAGAACGGGCAAAAAGCGGATGCGCTGGGG
CTGGATTTCCTGGGTATTCCGGTCACATTGTTGAATTACTCGTCATCGGTTATTCCCATT
ATTTTTTCTGCCTGGTTGTGCAGCATTCTGGAACGCCGACTTAATGCGTGGTTACCGTCG
GCAATCAAAAATTTCTTCACACCATTGCTATGTCTGATGGTTATCACACCCGTCACCTTT
CTGCTGGTGGGGCCGCTATCAACCTGGATAAGCGAACTGATTGCCGCCGGTTATCTCTGG
CTTTATCAGGCGGTTCCTGCATTTGCGGGCGCGGTAATGGGCGGCTTCTGGCAAATCTTC
GTCATGTTCGGACTGCACTGGGGCCTGGTGCCGCTGTGTATCAATAACTTCACCGTGCTG
GGCTACGACACCATGATCCCGCTGTTAATGCCCGCCATTATGGCGCAGGTCGGGGCGGCG
CTCGGCGTCTTCCTCTGCGAACGCGATGCGCAGAAAAAAGTGGTGGCGGGATCAGCGGCG
TTGACGAGTCTGTTTGGTATCACCGAACCAGCGGTATATGGCGTCAACCTGCCGCGTAAG
TACCCCTTTGTTATCGCCTGTATCAGTGGGGCTTTGGGGGCCACCATTATTGGCTACGCG
CAAACGAAAGTCTACTCCTTTGGTTTGCCAAGTATTTTCACCTTCATGCAAACCATCCCG
TCAACGGGAATTGATTTCACCGTCTGGGCCAGCGTTATTGGCGGTGTCATTGCCATCGGT
TGCGCATTTGTCGGTACGGTGATGCTTCATTTCATCACCGCTAAACGTCAGCCAGCGCAG
GGTGCCCCGCAAGAGAAAACACCAGAGGTTATTACACCACCTGAGCAGGGCGGTATCTGT
TCACCGATGACGGGAGAGATTGTGCCGCTCATTCACGTCGCTGATACCACGTTTGCCAGT
GGCCTGTTGGGTAAAGGTATTGCCATTCTGCCCTCGGTTGGTGAAGTGCGTTCTCCGGTT
GCGGGTCGAATTGCTTCGTTGTTCGCCACATTACACGCCATTGGCATTGAGTCAGATGAT
GGTGTGGAGATCCTGATTCATGTCGGTATCGACACCGTAAAACTGGACGGCAAATTCTTT
TCCGCTCACGTCAACGTGGGTGACAAGGTCAATACAGGCGATCGGCTGATTTCTTTTGAT
ATCCCTGCTATTCGCGAGGCCGGATTTGATCTGACGACGCCGGTATTAATCAGTAATAGC
GATGATTTTACGGACGTATTACCCCACGGCACGGCGCAGATAAGCGCAGGTGAACCGCTG
TTATCCATCATTCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:625
Protein Molecular Weight:66482
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 100-120
  • 141-161
  • 167-187
  • 203-223
  • 245-265
  • 285-305
  • 325-345
  • 354-374
  • 381-401
  • 404-424
  • 432-452
Protein Sequence:
>PTS system beta-glucoside-specific EIIBCA component
MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLKKTPGIIMVVESGGQFQ
VVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVYVISGIFTPLIGLMAATGI
LKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILGYTAGKRFGGNPFTAMVIGGA
LVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVIPIIFSAWLCSILERRLNAWLPS
AIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAAGYLWLYQAVPAFAGAVMGGFWQIF
VMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIMAQVGAALGVFLCERDAQKKVVAGSAA
LTSLFGITEPAVYGVNLPRKYPFVIACISGALGATIIGYAQTKVYSFGLPSIFTFMQTIP
STGIDFTVWASVIGGVIAIGCAFVGTVMLHFITAKRQPAQGAPQEKTPEVITPPEQGGIC
SPMTGEIVPLIHVADTTFASGLLGKGIAILPSVGEVRSPVAGRIASLFATLHAIGIESDD
GVEILIHVGIDTVKLDGKFFSAHVNVGDKVNTGDRLISFDIPAIREAGFDLTTPVLISNS
DDFTDVLPHGTAQISAGEPLLSIIR
References
External Links:
ResourceLink
Uniprot ID:P08722
Uniprot Name:PTV3B_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676316
Ecogene ID:EG10115
Ecocyc:EG10115
ColiBase:b3722
Kegg Gene:b3722
EchoBASE ID:EB0113
CCDB:PTV3B_ECOLI
BacMap:16131590
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bramley, H. F., Kornberg, H. L. (1987). "Nucleotide sequence of bglC, the gene specifying enzymeIIbgl of the PEP:sugar phosphotransferase system in Escherichia coli K12, and overexpression of the gene product." J Gen Microbiol 133:563-573. Pubmed: 3309161
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Schnetz, K., Toloczyki, C., Rak, B. (1987). "Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes." J Bacteriol 169:2579-2590. Pubmed: 3034860