Phenylalanyl-tRNA synthetase beta chain (P07395)

Identification
Name:Phenylalanyl-tRNA synthetase beta chain
Synonyms:
  • Phenylalanine--tRNA ligase beta chain
  • PheRS
Gene Name:pheT
Enzyme Class:
Biological Properties
General Function:Involved in RNA binding
Specific Function:ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Phe)+1.0tRNA(Phe) ↔ 1.0Thumb+1.0L-Phenylalanyl-tRNA(Phe)+1.0Thumb+1.0L-Phenylalanyl-tRNA(Phe)
1.0Adenosine triphosphate + 1.0L-Phenylalanine + 1.0tRNA(Phe) + 1.0tRNA(Phe) ↔ 1.0Adenosine monophosphate + 1.0L-Phenylalanyl-tRNA(Phe) + 1.0Pyrophosphate + 1.0L-Phenylalanyl-tRNA(Phe)
ReactionCard
1.0Thumb+1.0Thumb+1.0tRNA(Phe) ↔ 1.0Thumb+1.0Thumb+1.0L-Phenylalanyl-tRNA(Phe)
1.0Adenosine triphosphate + 1.0L-Phenylalanine + 1.0tRNA(Phe) ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-Phenylalanyl-tRNA(Phe)
ReactionCard
SMPDB Reactions:
1.0L-Phenylalanine+1.0Thumb+1.0Thumb+1.0tRNA(Phe)+1.0Thumb → 1.0Thumb+1.0Pyrophosphate+1.0L-phenylalanyl-tRNA(Phe)
1.0L-Phenylalanine + 1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0tRNA(Phe) + 1.0L-Phenylalanine → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0L-phenylalanyl-tRNA(Phe)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00159L-PhenylalanineMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
cation binding
ion binding
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
magnesium ion binding
metal ion binding
nucleic acid binding
nucleoside binding
nucleotide binding
phenylalanine-tRNA ligase activity
purine nucleoside binding
RNA binding
tRNA binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
phenylalanyl-tRNA aminoacylation
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
tRNA processing
Gene Properties
Blattner:b1713
Gene OrientationCounterclockwise
Centisome Percentage:38.66
Left Sequence End1793581
Right Sequence End1795968
Gene Sequence:
>2388 bp
ATGAAATTCAGTGAACTGTGGTTACGCGAATGGGTGAACCCGGCGATTGATAGCGATGCG
CTGGCAAATCAAATCACTATGGCGGGCCTGGAAGTTGACGGTGTAGAACCGGTTGCCGGC
AGCTTCCACGGCGTGGTCGTTGGTGAAGTGGTTGAGTGTGCGCAGCATCCGAACGCTGAC
AAACTGCGTGTGACAAAAGTGAATGTCGGCGGCGATCGCCTGCTGGACATCGTCTGCGGT
GCGCCAAACTGCCGTCAGGGCCTGCGTGTAGCGGTAGCGACCATTGGTGCTGTTCTGCCG
GGTGATTTCAAAATTAAAGCGGCGAAACTGCGTGGCGAACCGTCTGAAGGGATGCTGTGC
TCCTTCTCTGAACTGGGCATTTCTGACGATCACAGCGGCATTATCGAACTGCCTGCGGAT
GCGCCGATTGGCACCGATATCCGTGAATACCTGAAACTTGATGACAACACCATCGAAATC
AGCGTGACGCCAAACCGTGCCGACTGCTTAGGCATCATTGGTGTTGCGCGTGACGTTGCC
GTGCTGAACCAGCTGCCGCTGGTTCAACCGGAAATCGTTCCGGTTGGTGCGACCATCGAC
GACACGCTGCCGATTACAGTCGAAGCGCCGGAAGCCTGCCCGCGTTATCTTGGCCGTGTG
GTAAAAGGCATTAACGTTAAAGCGCCAACTCCGCTGTGGATGAAAGAAAAACTGCGTCGT
TGCGGGATCCGTTCTATCGATGCAGTTGTTGACGTCACCAACTATGTGCTGCTCGAACTG
GGCCAGCCGATGCACGCTTTCGATAAAGATCGCATTGAAGGCGGCATTGTGGTGCGGATG
GCGAAAGAGGGCGAAACGCTGGTGCTGCTCGACGGTACTGAAGCGAAGCTGAATGCTGAC
ACTCTGGTCATCGCCGACCACAACAAGGCGCTGGCGATGGGCGGCATCTTCGGTGGCGAA
CACTCTGGCGTGAATGACGAAACACAAAACGTGCTGCTGGAATGCGCGTTCTTTAGCCCG
CTGTCTATCACCGGTCGTGCTCGTCGTCATGGCCTGCATACCGATGCGTCTCACCGTTAT
GAGCGTGGCGTTGATCCGGCACTGCAGCACAAAGCGATGGAACGTGCGACCCGTCTGCTG
ATCGACATCTGCGGTGGTGAGGCTGGCCCGGTAATTGATATCACCAACGAAGCAACGCTG
CCGAAGCGTGCAACCATCACTCTACGTCGTAGCAAACTGGATCGCCTGATCGGCCATCAT
ATTGCGGATGAGCAGGTAACTGACATTCTGCGTCGTCTCGGCTGCGAAGTGACCGAAGGC
AAAGACGAATGGCAGGCAGTTGCGCCGAGCTGGCGTTTCGATATGGAGATTGAAGAAGAT
CTGGTTGAAGAAGTCGCGCGTGTTTACGGCTACAACAACATCCCGGATGAGCCGGTACAG
GCAAGCCTGATTATGGGTACTCACCGTGAAGCTGACCTGTCGCTCAAGCGCGTGAAAACG
CTGCTCAACGACAAAGGCTATCAGGAAGTGATCACCTACAGCTTCGTTGATCCGAAAGTG
CAGCAGATGATCCATCCAGGCGTTGAAGCCTTACTGCTGCCAAGCCCGATCTCTGTTGAA
ATGTCAGCAATGCGTCTTTCTCTGTGGACTGGCCTGCTGGCAACCGTGGTGTACAACCAG
AACCGTCAGCAGAACCGTGTGCGCATTTTCGAAAGCGGTCTGCGTTTCGTACCAGATACT
CAGGCACCGTTGGGCATTCGTCAGGATCTGATGTTAGCCGGTGTGATTTGCGGTAACCGT
TACGAAGAGCACTGGAACCTGGCAAAAGAGACCGTTGATTTCTATGATTTGAAAGGCGAT
CTTGAATCCGTTCTCGACCTGACCGGTAAACTGAATGAGGTTGAGTTCCGTGCAGAAGCG
AATCCGGCACTGCATCCGGGGCAATCCGCAGCGATTTATCTGAAAGGTGAACGTATTGGT
TTTGTTGGGGTTGTTCATCCTGAACTGGAACGTAAACTGGATCTTAACGGTCGCACTCTG
GTGTTCGAACTGGAGTGGAACAAGCTCGCAGACCGCGTGGTGCCTCAGGCGCGCGAGATT
TCTCGCTTCCCGGCGAACCGTCGTGACATCGCGGTGGTGGTCGCAGAAAACGTTCCCGCA
GCGGATATTTTATCCGAATGTAAGAAAGTTGGCGTAAATCAGGTAGTTGGCGTAAACTTA
TTTGACGTGTACCGCGGTAAGGGTGTTGCGGAGGGGTATAAGAGCCTCGCCATAAGCCTG
ATCCTGCAAGATACCAGCCGTACACTCGAAGAAGAGGAGATTGCCGCTACCGTCGCCAAA
TGTGTAGAGGCATTAAAAGAGCGATTCCAGGCATCATTGAGGGATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:795
Protein Molecular Weight:87377
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phenylalanyl-tRNA synthetase beta chain
MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGVVVGEVVECAQHPNAD
KLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFKIKAAKLRGEPSEGMLC
SFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVTPNRADCLGIIGVARDVA
VLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVKGINVKAPTPLWMKEKLRR
CGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMAKEGETLVLLDGTEAKLNAD
TLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSPLSITGRARRHGLHTDASHRY
ERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEATLPKRATITLRRSKLDRLIGHH
IADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIEEDLVEEVARVYGYNNIPDEPVQ
ASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVDPKVQQMIHPGVEALLLPSPISVE
MSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNR
YEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIG
FVGVVHPELERKLDLNGRTLVFELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPA
ADILSECKKVGVNQVVGVNLFDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAK
CVEALKERFQASLRD
References
External Links:
ResourceLink
Uniprot ID:P07395
Uniprot Name:SYFB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742793
Ecogene ID:EG10710
Ecocyc:EG10710
ColiBase:b1713
Kegg Gene:b1713
EchoBASE ID:EB0704
CCDB:SYFB_ECOLI
BacMap:16129669
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mechulam, Y., Fayat, G., Blanquet, S. (1985). "Sequence of the Escherichia coli pheST operon and identification of the himA gene." J Bacteriol 163:787-791. Pubmed: 2991205