Bifunctional protein BirA (P06709)

Identification
Name:Bifunctional protein BirA
Synonyms:
  • Biotin operon repressor
  • Biotin--[acetyl-CoA-carboxylase] synthetase
  • Biotin--protein ligase
Gene Name:birA
Enzyme Class:
Biological Properties
General Function:Involved in biotin-[acetyl-CoA-carboxylase] ligase activity
Specific Function:BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting proteins
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Biotin ↔ 1.0Pyrophosphate + 1.0Biotinyl-5'-AMP
ReactionCard
1.0Thumb+1.0Apo-[carboxylase] ↔ 1.0Thumb+1.0Holo-[carboxylase]
1.0Biotinyl-5'-AMP + 1.0Apo-[carboxylase] ↔ 1.0Adenosine monophosphate + 1.0Holo-[carboxylase]
ReactionCard
1.0Thumb+1.0Thumb+1.0Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ 1.0Thumb+1.0Thumb+1.0[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
1.0Adenosine triphosphate + 1.0Biotin + 1.0Apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0[Acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0diphosphate+1.0Thumb+1.0Thumb
1.0Biotin + 1.0Adenosine triphosphate → 1.0diphosphate + 1.0Biotinyl-5'-AMP + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0apo-[carboxylase] → 1.0Thumb+1.0Biotin-Carboxyl Carrying Protein
1.0Biotinyl-5'-AMP + 1.0apo-[carboxylase] → 1.0Adenosine monophosphate + 1.0Biotin-Carboxyl Carrying Protein
ReactionCard
1.0Thumb+1.0Thumb+1.0Biotin-Carboxyl Carrying Protein → 1.0Thumb+1.0Thumb+1.0Pyrophosphate+1.0Biotinylated [BCCP monomer]
1.0Adenosine triphosphate + 1.0Biotin + 1.0Biotin-Carboxyl Carrying Protein → 1.0Hydrogen ion + 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0Biotinylated [BCCP monomer]
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → 1.0Thumb+1.0Thumb+1.0[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
1.0Adenosine triphosphate + 1.0Biotin + 1.0apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB00030BiotinMetaboCard
ECMDB04220Biotinyl-5'-AMPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
biotin-protein ligase activity
biotin-[acetyl-CoA-carboxylase] ligase activity
catalytic activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
transcription regulator activity
transcription repressor activity
Process
biological regulation
macromolecule metabolic process
macromolecule modification
metabolic process
protein modification process
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
Gene Properties
Blattner:b3973
Gene OrientationClockwise
Centisome Percentage:89.90
Left Sequence End4171105
Right Sequence End4172070
Gene Sequence:
>966 bp
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATG
AGTCTGCAACAGGAATTACGCTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAG
AAAATGCTGGAAGCGGGCGAAAGTGCGCTGGATGTGGTGACGGAAGCGGTGCGTCTGCTG
GAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCTGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTT
AGTCATCTGCGTAATCCGGTTCTTGCCGCCCGGCTGGTGATGGAGCAAAGCCCGCATGTG
ATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTCGTGGCATGGAGCGCGTCTCG
CCGGAGATTTTCTCCACGTCTTTGCGTTATGAACAACTACTGGCAGCGCGCAAGGAAGGG
GCAACCGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTG
GGGGCCGTGGCGTTGGATTTAGACGGCAATTTGGCGGCAGCCACGTCCACAGGCGGAATG
ACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAGTGGGTGCCGGATGCTACGCC
AATAACGCCAGTGTGGCGGTTTCTTGTACCGGCACGGGCGAAGTCTTCATCCGCGCGCTG
GCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGC
GAGCGGGTAGTAATGGAAAAACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATC
GACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAGGAATGTATCGCGCCTGGGGC
TACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACA
CAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:321
Protein Molecular Weight:35312
Protein Theoretical pI:8
PDB File:1HXD
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Bifunctional protein BirA
MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSL
PEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGRRG
RKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQ
DRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAA
MLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLL
EQDGIIKPWMGGEISLRSAEK
References
External Links:
ResourceLink
Uniprot ID:P06709
Uniprot Name:BIRA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062400
PDB ID:1HXD
Ecogene ID:EG10123
Ecocyc:EG10123
ColiBase:b3973
Kegg Gene:b3973
EchoBASE ID:EB0121
CCDB:BIRA_ECOLI
BacMap:16131807
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Buoncristiani, M. R., Howard, P. K., Otsuka, A. J. (1986). "DNA-binding and enzymatic domains of the bifunctional biotin operon repressor (BirA) of Escherichia coli." Gene 44:255-261. Pubmed: 3536662
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Howard, P. K., Shaw, J., Otsuka, A. J. (1985). "Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli." Gene 35:321-331. Pubmed: 3899863
  • Pucci, M. J., Discotto, L. F., Dougherty, T. J. (1992). "Cloning and identification of the Escherichia coli murB DNA sequence, which encodes UDP-N-acetylenolpyruvoylglucosamine reductase." J Bacteriol 174:1690-1693. Pubmed: 1311302
  • Song, W. J., Jackowski, S. (1992). "Cloning, sequencing, and expression of the pantothenate kinase (coaA) gene of Escherichia coli." J Bacteriol 174:6411-6417. Pubmed: 1328157
  • Weaver, L. H., Kwon, K., Beckett, D., Matthews, B. W. (2001). "Corepressor-induced organization and assembly of the biotin repressor: a model for allosteric activation of a transcriptional regulator." Proc Natl Acad Sci U S A 98:6045-6050. Pubmed: 11353844
  • Wilson, K. P., Shewchuk, L. M., Brennan, R. G., Otsuka, A. J., Matthews, B. W. (1992). "Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains." Proc Natl Acad Sci U S A 89:9257-9261. Pubmed: 1409631