Glucitol/sorbitol-specific phosphotransferase enzyme IIA component (P05706)

Identification
Name:Glucitol/sorbitol-specific phosphotransferase enzyme IIA component
Synonyms:
  • EIIA-Gut
  • EIII-Gut
  • PTS system glucitol/sorbitol-specific EIIA component
Gene Name:srlB
Enzyme Class:
Biological Properties
General Function:Involved in protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucitol/sorbitol transport
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Thumb ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sorbitol ↔ 1.0Protein histidine + 1.0Sorbitol-6-phosphate
ReactionCard
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Sorbitol → 1.0Pyruvic acid + 1.0Sorbitol-6-phosphate
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB → 1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00247SorbitolMetaboCard
ECMDB23836Sorbitol 6-phosphateMetaboCard
ECMDB01400Sorbitol-6-phosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
carbohydrate transmembrane transporter activity
protein-N(PI)-phosphohistidine-sugar phosphotransferase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b2704
Gene OrientationClockwise
Centisome Percentage:60.90
Left Sequence End2825384
Right Sequence End2825755
Gene Sequence:
>372 bp
ATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCT
CATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAA
GCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAAGGCACC
CGGGTCAGCATTAATAATGCCAGCGCGGAAGAGCTAGCCCGCGCGATGAATGGCGTTGGC
CTGAAGAAAGCGCAGGCGATTGTCAGTTATCGCGAAGAGTACGGTCCGTTTAAAACTGTG
GAGGATCTAAAGCAGGTGCCGGGGATGGGCAATTCGCTGGTGGAACGTAATCTGGCGGTA
TTAACCCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:123
Protein Molecular Weight:13304
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucitol/sorbitol-specific phosphotransferase enzyme IIA component
MTVIYQTTITRIGASAIDALSDQMLITFREGAPADLEEYCFIHCHGELKGALHPGLQFSL
GQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHVAGPVPDDIAPGSVLKFES
VKE
References
External Links:
ResourceLink
Uniprot ID:P05706
Uniprot Name:PTHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674582
Ecogene ID:EG10970
Ecocyc:EG10970
ColiBase:b2704
Kegg Gene:b2704
EchoBASE ID:EB0963
CCDB:PTHA_ECOLI
BacMap:16130611
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hall, B. G., Sharp, P. M. (1992). "Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates." Mol Biol Evol 9:654-665. Pubmed: 1630305
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamada, M., Saier, M. H. Jr (1987). "Glucitol-specific enzymes of the phosphotransferase system in Escherichia coli. Nucleotide sequence of the gut operon." J Biol Chem 262:5455-5463. Pubmed: 3553176
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837