3-deoxy-manno-octulosonate cytidylyltransferase (P04951)

Identification
Name:3-deoxy-manno-octulosonate cytidylyltransferase
Synonyms:
  • CMP-2-keto-3-deoxyoctulosonic acid synthase
  • CKS
  • CMP-KDO synthase
Gene Name:kdsB
Enzyme Class:
Biological Properties
General Function:Involved in lipopolysaccharide biosynthetic process
Specific Function:Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.03-Deoxy-D-manno-octulosonate ↔ 1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0Pyrophosphate
ReactionCard
SMPDB Reactions:
1.03-deoxy-D-manno-octulosonate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.03-deoxy-D-manno-octulosonate + 1.0Cytidine triphosphate + 1.03-Deoxy-D-manno-octulosonate → 1.0Pyrophosphate + 1.0CMP-3-Deoxy-D-manno-octulosonate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Cytidine triphosphate + 1.03-Deoxy-D-manno-octulosonate ↔ 1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Deoxy-D-manno-octulosonate + 1.0Cytidine triphosphate → 1.0CMP-3-Deoxy-D-manno-octulosonate + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200693-Deoxy-D-manno-octulosonateMetaboCard
ECMDB20133CMP-3-Deoxy-D-manno-octulosonateMetaboCard
ECMDB00082Cytidine triphosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
3-deoxy-manno-octulosonate cytidylyltransferase activity
catalytic activity
cytidylyltransferase activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b0918
Gene OrientationClockwise
Centisome Percentage:20.91
Left Sequence End970075
Right Sequence End970821
Gene Sequence:
>747 bp
ATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCC
ACGGCGCTGGCGTTTGTTGAGGCGGGAGCGAAAGTTACAGGTTTTGATCAAGCGTTCACT
CAGGAGCAATATCCCTTTGCGACCGAAGTGATGGATGTTGCCGACGCTGCGCAGGTCGCG
CAAGTGTGTCAGCGACTGTTAGCTGAAACGGAGCGACTGGACGCGCTGGTCAATGCGGCG
GGAATTTTACGCATGGGCGCGACCGATCAGCTCAGTAAAGAGGACTGGCAGCAGACTTTT
GCGGTTAACGTCGGCGGTGCGTTTAACCTGTTCCAGCAAACCATGAACCAGTTTCGCCGT
CAGCGGGGCGGGGCGATTGTCACTGTGGCGTCCGACGCCGCGCACACGCCGCGTATTGGC
ATGAGTGCTTATGGCGCATCGAAAGCGGCGCTGAAAAGCCTGGCGTTGAGCGTCGGGCTG
GAACTGGCGGGTAGCGGCGTGCGCTGTAATGTGGTTTCGCCTGGCTCCACCGACACCGAT
ATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGC
GAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAAC
ACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTC
GATGGCGGCTCAACGCTGGGGGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:248
Protein Molecular Weight:27614
Protein Theoretical pI:5
PDB File:1VH1
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-deoxy-manno-octulosonate cytidylyltransferase
MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAERIIVATDHEDVARAVEA
AGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEPMIPATIIRQVADNLAQRQ
VGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATIPWDRDRFAEGLETVGDNFLR
HLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYGEKIHVAVAQEVPGTGVDTPEDL
ERVRAEMR
References
External Links:
ResourceLink
Uniprot ID:P04951
Uniprot Name:KDSB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674717
PDB ID:1VH1
Ecogene ID:EG10519
Ecocyc:EG10519
ColiBase:b0918
Kegg Gene:b0918
EchoBASE ID:EB0514
CCDB:KDSB_ECOLI
BacMap:16128885
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Goldman, R. C., Bolling, T. J., Kohlbrenner, W. E., Kim, Y., Fox, J. L. (1986). "Primary structure of CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) from Escherichia coli." J Biol Chem 261:15831-15835. Pubmed: 3023327
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232