| Identification |
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| Name: | Glutathione-regulated potassium-efflux system protein kefC |
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| Synonyms: | |
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| Gene Name: | kefC |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | Involved in solute:hydrogen antiporter activity |
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| Specific Function: | Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport |
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| Cellular Location: | Cell inner membrane; Multi-pass membrane protein |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Transports: | |
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| Transport References: | - Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
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| Metabolites: | |
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| GO Classification: | | Component |
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| cell part | | integral to membrane | | intrinsic to membrane | | membrane part | | Function |
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| binding | | catalytic activity | | cation transmembrane transporter activity | | ion transmembrane transporter activity | | solute:cation antiporter activity | | solute:hydrogen antiporter activity | | substrate-specific transmembrane transporter activity | | transmembrane transporter activity | | transporter activity | | Process |
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| cation transport | | establishment of localization | | ion transport | | metabolic process | | monovalent inorganic cation transport | | potassium ion transport | | transmembrane transport | | transport |
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| Gene Properties |
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| Blattner: | b0047 |
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| Gene Orientation | Clockwise |
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| Centisome Percentage: | 1.03 |
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| Left Sequence End | 47769 |
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| Right Sequence End | 49631 |
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| Gene Sequence: | >1863 bp
ATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTA
CCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATT
ATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAG
ATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGG
AAGCTGCGTGCGGCAGTGTTCGGCTGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTG
CTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATG
ACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATG
GTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATC
CCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCA
TTTGCTCTCTCGGCGTTAAAAGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGC
TATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGT
GCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCG
ATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCG
CTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTT
GGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTG
CTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTG
CCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTT
GTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTG
ACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCAATCGC
CTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCG
CGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTACCGGACGTTTACTGCTCTCC
AGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAA
TTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGCCGGA
GCGGCGAAAGCGGAAGTGCTGATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTG
ACAGAGATGGTGAAAGAACATTTCCCGCATTTGCAGATTATTGCCCGCGCCCGCGATGTC
GACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAA
GGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCCGTATGAAGCG
CGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATG
GTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGT
GAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGA
ACCGAAGAAGGTAAACATACCGGCAACATGGCGGATGAACCGGAAACGAAACCCTCATCC
TAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 620 |
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| Protein Molecular Weight: | 67795 |
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| Protein Theoretical pI: | 6 |
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| Signaling Regions: | |
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| Transmembrane Regions: | - 4-24
- 26-46
- 54-74
- 90-110
- 114-134
- 149-169
- 178-198
- 214-233
- 237-254
- 270-290
- 294-314
- 327-347
- 359-379
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| Protein Sequence: | >Glutathione-regulated potassium-efflux system protein kefC
MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPWGLRLVTDAESILHFAE
IGVVLMLFIIGLELDPQRLWKLRAAVFGCGALQMVICGGLLGLFCMLLGLRWQVAELIGM
TLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAIPLVAMIPLLATSSASTTMGA
FALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREVFSAVALFLVFGFGLLLEEVGLS
MAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLFFIGVGMSIDFGTLLENPLRIVILL
LGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQGSEFAFVVFGAAQMANVLEPEWAKSL
TLAVALSMAATPILLVILNRLEQSSTEEAREADEIDEEQPRVIIAGFGRFGQITGRLLLS
SGVKMVVLDHDPDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQL
TEMVKEHFPHLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEA
RERADVFRRFNIQMVEEMAMVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQG
TEEGKHTGNMADEPETKPSS |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Munro, A. W., Ritchie, G. Y., Lamb, A. J., Douglas, R. M., Booth, I. R. (1991). "The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli." Mol Microbiol 5:607-616. Pubmed: 2046548
- Reizer, J., Reizer, A., Saier, M. H. Jr (1992). "The putative Na+/H+ antiporter (NapA) of Enterococcus hirae is homologous to the putative K+/H+ antiporter (KefC) of Escherichia coli." FEMS Microbiol Lett 73:161-163. Pubmed: 1325937
- Smith, D. R., Calvo, J. M. (1980). "Nucleotide sequence of the E coli gene coding for dihydrofolate reductase." Nucleic Acids Res 8:2255-2274. Pubmed: 6159575
- Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901
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