Glutathione-regulated potassium-efflux system protein kefC (P03819)

Identification
Name:Glutathione-regulated potassium-efflux system protein kefC
Synonyms:
  • K(+)/H(+) antiporter
Gene Name:kefC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in solute:hydrogen antiporter activity
Specific Function:Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04139PotassiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
binding
catalytic activity
cation transmembrane transporter activity
ion transmembrane transporter activity
solute:cation antiporter activity
solute:hydrogen antiporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
metabolic process
monovalent inorganic cation transport
potassium ion transport
transmembrane transport
transport
Gene Properties
Blattner:b0047
Gene OrientationClockwise
Centisome Percentage:1.03
Left Sequence End47769
Right Sequence End49631
Gene Sequence:
>1863 bp
ATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTA
CCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATT
ATTGGCCCGTGGGGGCTGCGACTGGTGACCGATGCCGAATCTATTCTGCACTTTGCCGAG
ATTGGGGTGGTGCTGATGCTGTTTATTATCGGCCTCGAACTCGATCCACAAAGGCTGTGG
AAGCTGCGTGCGGCAGTGTTCGGCTGTGGCGCATTGCAGATGGTGATTTGCGGCGGCCTG
CTGGGGCTGTTCTGCATGTTACTTGGGCTGCGCTGGCAGGTCGCGGAATTGATCGGCATG
ACGCTGGCGCTCTCCTCTACGGCGATTGCCATGCAGGCGATGAATGAACGCAATCTGATG
GTGACGCAAATGGGTCGCAGTGCCTTTGCGGTGCTGCTGTTCCAGGATATCGCGGCGATC
CCGCTGGTGGCGATGATTCCGCTACTGGCAACGAGCAGTGCCTCGACGACGATGGGCGCA
TTTGCTCTCTCGGCGTTAAAAGTGGCGGGTGCGCTGGTGCTGGTGGTATTGCTGGGGCGC
TATGTCACGCGTCCGGCGCTGCGTTTTGTAGCCCGCTCTGGCTTGCGGGAAGTGTTTAGT
GCCGTGGCGTTATTCCTCGTGTTTGGCTTTGGTTTGCTGCTGGAAGAGGTCGGCTTGTCG
ATGGCGATGGGCGCGTTTCTGGCGGGCGTACTGCTGGCAAGCTCGGAATACCGTCATGCG
CTGGAGAGCGATATCGAACCATTTAAAGGTTTGCTGTTGGGGCTGTTTTTCATCGGTGTT
GGCATGTCGATAGACTTTGGCACGCTGCTTGAAAACCCATTGCGCATTGTCATTTTGCTG
CTCGGTTTCCTCATCATCAAAATCGCCATGCTGTGGCTGATTGCCCGACCGTTGCAAGTG
CCAAATAAACAGCGTCGTTGGTTTGCGGTGTTGTTAGGGCAGGGCAGTGAGTTTGCCTTT
GTGGTATTTGGCGCGGCGCAGATGGCGAATGTGCTGGAGCCGGAGTGGGCGAAATCGCTG
ACCCTGGCGGTGGCGCTGTCGATGGCAGCAACGCCGATTCTGCTGGTGATCCTCAATCGC
CTTGAGCAATCTTCTACTGAGGAAGCGCGTGAAGCCGATGAGATCGACGAAGAACAGCCG
CGCGTGATTATCGCCGGATTCGGTCGTTTTGGGCAGATTACCGGACGTTTACTGCTCTCC
AGCGGGGTGAAAATGGTGGTACTCGATCACGATCCGGACCATATCGAAACCTTGCGTAAA
TTTGGTATGAAAGTGTTTTATGGCGATGCCACGCGGATGGATTTACTGGAATCTGCCGGA
GCGGCGAAAGCGGAAGTGCTGATTAACGCCATCGACGATCCGCAAACCAACCTGCAACTG
ACAGAGATGGTGAAAGAACATTTCCCGCATTTGCAGATTATTGCCCGCGCCCGCGATGTC
GACCACTACATTCGTTTGCGTCAGGCAGGCGTTGAAAAGCCGGAGCGTGAAACCTTCGAA
GGTGCGCTGAAAACCGGGCGTCTGGCACTGGAAAGTTTAGGTCTGGGGCCGTATGAAGCG
CGAGAACGTGCCGATGTGTTCCGCCGCTTTAATATTCAGATGGTGGAAGAGATGGCAATG
GTTGAGAACGACACCAAAGCCCGCGCGGCGGTCTATAAACGCACCAGCGCGATGTTAAGT
GAGATCATTACCGAGGACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGA
ACCGAAGAAGGTAAACATACCGGCAACATGGCGGATGAACCGGAAACGAAACCCTCATCC
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:620
Protein Molecular Weight:67795
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 4-24
  • 26-46
  • 54-74
  • 90-110
  • 114-134
  • 149-169
  • 178-198
  • 214-233
  • 237-254
  • 270-290
  • 294-314
  • 327-347
  • 359-379
Protein Sequence:
>Glutathione-regulated potassium-efflux system protein kefC
MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPWGLRLVTDAESILHFAE
IGVVLMLFIIGLELDPQRLWKLRAAVFGCGALQMVICGGLLGLFCMLLGLRWQVAELIGM
TLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAIPLVAMIPLLATSSASTTMGA
FALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREVFSAVALFLVFGFGLLLEEVGLS
MAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLFFIGVGMSIDFGTLLENPLRIVILL
LGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQGSEFAFVVFGAAQMANVLEPEWAKSL
TLAVALSMAATPILLVILNRLEQSSTEEAREADEIDEEQPRVIIAGFGRFGQITGRLLLS
SGVKMVVLDHDPDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQL
TEMVKEHFPHLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEA
RERADVFRRFNIQMVEEMAMVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQG
TEEGKHTGNMADEPETKPSS
References
External Links:
ResourceLink
Uniprot ID:P03819
Uniprot Name:KEFC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321928
Ecogene ID:EG10521
Ecocyc:EG10521
ColiBase:b0047
Kegg Gene:b0047
EchoBASE ID:EB0516
CCDB:KEFC_ECOLI
BacMap:16128041
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Munro, A. W., Ritchie, G. Y., Lamb, A. J., Douglas, R. M., Booth, I. R. (1991). "The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli." Mol Microbiol 5:607-616. Pubmed: 2046548
  • Reizer, J., Reizer, A., Saier, M. H. Jr (1992). "The putative Na+/H+ antiporter (NapA) of Enterococcus hirae is homologous to the putative K+/H+ antiporter (KefC) of Escherichia coli." FEMS Microbiol Lett 73:161-163. Pubmed: 1325937
  • Smith, D. R., Calvo, J. M. (1980). "Nucleotide sequence of the E coli gene coding for dihydrofolate reductase." Nucleic Acids Res 8:2255-2274. Pubmed: 6159575
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901