Lactose permease (P02920)

Identification
Name:Lactose permease
Synonyms:
  • Lactose-proton symport
Gene Name:lacY
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transport
Specific Function:Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system)
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00186alpha-LactoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Process
establishment of localization
transport
Gene Properties
Blattner:b0343
Gene OrientationCounterclockwise
Centisome Percentage:7.78
Left Sequence End361150
Right Sequence End362403
Gene Sequence:
>1254 bp
ATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCC
AGCCGCGAAAAAAATCGCGTGCTGGAAAAAATCGCCGATGAACTGGAAGCACAAAGCGAA
ATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAA
GCGATGCTTGACCGTCTGGCACTGACGCCCGCACGGCTGAAAGGCATTGCCGACGATGTA
CGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTACTGGAC
AGCGGCCTGCGTCTTGAGCGTCGTCGCGTACCGCTGGGGGTTATTGGCGTGATTTATGAA
GCGCGCCCGAACGTGACGGTTGATGTCGCTTCGCTGTGCCTGAAAACCGGTAATGCGGTG
ATCCTGCGCGGTGGCAAAGAAACGTGTCGCACTAACGCTGCAACGGTGGCGGTGATTCAG
GACGCCCTGAAATCCTGCGGCTTACCGGCGGGTGCCGTGCAGGCGATTGATAATCCTGAC
CGTGCGCTGGTCAGTGAAATGCTGCGTATGGATAAATACATCGACATGCTGATCCCGCGT
GGTGGCGCTGGTTTGCATAAACTGTGCCGTGAACAGTCGACAATCCCGGTGATCACAGGT
GGTATAGGCGTATGCCATATTTACGTTGATGAAAGTGTAGAGATCGCTGAAGCATTAAAA
GTGATCGTCAACGCGAAAACTCAGCGTCCGAGCACATGTAATACGGTTGAAACGTTGCTG
GTGAATAAAAACATCGCCGATAGCTTCCTGCCCGCATTAAGCAAACAAATGGCGGAAAGC
GGCGTGACATTACACGCAGATGCAGCTGCACTGGCGCAGTTGCAGGCAGGCCCTGCGAAG
GTGGTTGCTGTTAAAGCCGAAGAGTATGACGATGAGTTTCTGTCATTAGATTTGAACGTC
AAAATCGTCAGCGATCTTGACGATGCCATCGCCCATATTCGTGAACACGGCACACAACAC
TCCGATGCGATCCTGACCCGCGATATGCGCAACGCCCAGCGTTTTGTTAACGAAGTGGAT
TCGTCCGCTGTTTACGTTAACGCCTCTACGCGTTTTACCGACGGCGGCCAGTTTGGTCTG
GGTGCGGAAGTGGCGGTAAGCACACAAAAACTCCACGCGCGTGGCCCAATGGGGCTGGAA
GCACTGACCACTTACAAGTGGATCGGCATTGGTGATTACACCATTCGTGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:417
Protein Molecular Weight:46503
Protein Theoretical pI:9
PDB File:1PV7
Signaling Regions:
  • None
Transmembrane Regions:
  • 8-34
  • 42-70
  • 75-100
  • 105-129
  • 141-163
  • 167-186
  • 221-249
  • 254-278
  • 289-308
  • 312-334
  • 347-374
  • 378-398
Protein Sequence:
>Lactose permease
MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQ
PLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNA
GAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALIL
AVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFD
QQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMS
VRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQL
AMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA
References
External Links:
ResourceLink
Uniprot ID:P02920
Uniprot Name:LACY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674395
PDB ID:1PV7
Ecogene ID:EG10526
Ecocyc:EG10526
ColiBase:b0343
Kegg Gene:b0343
EchoBASE ID:EB0521
CCDB:LACY_ECOLI
BacMap:16128328
General Reference:
  • Abramson, J., Smirnova, I., Kasho, V., Verner, G., Kaback, H. R., Iwata, S. (2003). "Structure and mechanism of the lactose permease of Escherichia coli." Science 301:610-615. Pubmed: 12893935
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Buchel, D. E., Gronenborn, B., Muller-Hill, B. (1980). "Sequence of the lactose permease gene." Nature 283:541-545. Pubmed: 6444453
  • Calamia, J., Manoil, C. (1990). "lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion." Proc Natl Acad Sci U S A 87:4937-4941. Pubmed: 2164211
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heijne, G. (1986). "The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology." EMBO J 5:3021-3027. Pubmed: 16453726
  • Huang, A. M., Lee, J. I., King, S. C., Wilson, T. H. (1992). "Amino acid substitution in the lactose carrier protein with the use of amber suppressors." J Bacteriol 174:5436-5441. Pubmed: 1644770
  • Kaback, H. R. (1990). "The lac permease of Escherichia coli: a prototypic energy-transducing membrane protein." Biochim Biophys Acta 1018:160-162. Pubmed: 2203471
  • King, S. C., Hansen, C. L., Wilson, T. H. (1991). "The interaction between aspartic acid 237 and lysine 358 in the lactose carrier of Escherichia coli." Biochim Biophys Acta 1062:177-186. Pubmed: 1848449
  • Ujwal, M. L., Jung, H., Bibi, E., Manoil, C., Altenbach, C., Hubbell, W. L., Kaback, H. R. (1995). "Membrane topology of helices VII and XI in the lactose permease of Escherichia coli studied by lacY-phoA fusion analysis and site-directed spectroscopy." Biochemistry 34:14909-14917. Pubmed: 7578103
  • Whitelegge, J. P., le Coutre, J., Lee, J. C., Engel, C. K., Prive, G. G., Faull, K. F., Kaback, H. R. (1999). "Toward the bilayer proteome, electrospray ionization-mass spectrometry of large, intact transmembrane proteins." Proc Natl Acad Sci U S A 96:10695-10698. Pubmed: 10485888