Displaying growth conditions 201 - 225 of 1187 in total
ECMDB IDName ConcentrationStrain
Media
Growth Status
Growth System
TemperatureCitation
ECMDB00687L-Leucine202± 26 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00687L-Leucine224± 24 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00172L-Isoleucine150± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00172L-Isoleucine220± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00172L-Isoleucine170± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00172L-Isoleucine33± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00172L-Isoleucine172± 23 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00172L-Isoleucine178± 23 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB02251L-Homoserine16± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00165L-Homocysteine370± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03431L-Histidinol13± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03431L-Histidinol19± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB03431L-Histidinol20± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00177L-Histidine68± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00177L-Histidine175± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00177L-Histidine98± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00177L-Histidine52± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00177L-Histidine235± 26 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00177L-Histidine0.22± 0.01 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00641L-Glutamine3810± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00641L-Glutamine4950± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00641L-Glutamine3060± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L acetate
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00641L-Glutamine494± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00641L-Glutamine184± 23 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
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ECMDB00641L-Glutamine249± 20 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
Displaying growth conditions 201 - 225 of 1187 in total