ECMDB

Identification

Name:

Vitamin B12 transporter BtuB

Synonyms:

Cobalamin receptor
Outer membrane cobalamin translocator

Gene Name:

btuB

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in receptor activity

Specific Function:

Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein tonB

Cellular Location:

Cell outer membrane; Multi-pass membrane protein

SMPDB Pathways:

adenosylcobalamin salvage from cobinamide PW001884

KEGG Pathways:

Not Available

Transports:

Transport References:

Not Available

Metabolites:

ECMDB ID	Name	View

GO Classification:

Component
cell part
membrane
Function
molecular transducer activity
receptor activity
signal transducer activity
transporter activity
Process
cobalamin transport
establishment of localization
transport
vitamin transport

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>1845 bp
ATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCTGTCACAGCCTTTTCCGCTTGGGCA
CAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGC
ACTGTGCTTGCACCAACCACCGTTGTGACGCGTCAGGATATCGACCGCTGGCAGTCGACC
TCGGTTAATGATGTGCTGCGCCGTCTTCCGGGCGTCGATATCACCCAAAACGGCGGTTCA
GGTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATT
GATGGCGTACGCCTGAATCTGGCGGGGGGGAGTGGTTCTGCCGACCTTAGCCAGTTCCCT
ATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCACGCTCCGCCGTTTATGGTTCC
GATGCAATAGGTGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATT
TCAGCAGGGTGGGGAAGCAATAGTTATCAAAACTATGATGTCTCTACACAGCAACAACTG
GGGGATAAGACACGAGTAACGTTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTT
GTTGCCTATGGTAATACCGGAACGCAAGCGCAGCCAGATAACGATGGTTTTTTAAGTAAA
ACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGC
TATGGCTATGATAACCGTACCAATTATGACGCGTATTATTCTCCGGGTTCACCATTGGTC
GATACCCGTAAACTCTATAGTCAAAGTTGGGACGCCGGGCTGCGATATAACGGCGAACTG
ATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCAT
TATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGG
GCAAACAACATCATCATTGGCCACGGTAATGTTGGTGCGGGTGTTGACTGGCAGAAGCAG
AGCACGGCACCGGGCACAGCTTATGTTAAGGATGGATATGATCAACGTAATACCGGCATC
TATCTGACCGGGCTGCAACAAGTCGGCGATTTTACCTTTGAAGGCGCAGCACGCAGCGAC
GATAACTCACAGTTTGGTCGTCATGGAACCTGGCAAACCAGCGCCGGTTGGGAATTCATC
GAAGGTTATCGCTTCATTGCTTCCTACGGGACATCTTATAAGGCACCAAATCTGGGGCAA
CTGTATGGCTTCTACGGAAATCCGAATCTGGACCCGGAGAAAAGCAAACAGTGGGAAGGC
GCGTTTGAAGGCTTAACCGCTGGGGTGAACTGGCGTATTTCCGGATATCGTAACGATGTC
AGTGACTTGATCGATTATGATGATCACACCCTGAAATATTACAACGAAGGGAAAGCGCGG
ATTAAGGGCGTCGAGGCGACCGCCAATTTTGATACCGGACCACTGACGCATACTGTGAGT
TATGATTATGTCGATGCGCGCAATGCAATTACCGACACGCCGTTGTTACGCCGTGCTAAA
CAGCAGGTGAAATACCAGCTCGACTGGCAGTTGTATGACTTCGACTGGGGTATTACTTAT
CAGTATTTAGGCACTCGCTATGATAAGGATTACTCATCTTATCCTTATCAAACCGTTAAA
ATGGGCGGTGTGAGCTTGTGGGATCTTGCGGTTGCGTATCCGGTCACCTCTCACCTGACA
GTTCGTGGTAAAATAGCCAACCTGTTCGACAAAGATTATGAGACAGTCTATGGCTACCAA
ACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGA

Protein Properties

Pfam Domain Function:

Plug (PF07715 )
TonB_dep_Rec (PF00593 )

Protein Residues:

614

Protein Molecular Weight:

68357

Protein Theoretical pI:

PDB File:

1UJW

Signaling Regions:

1-20

Transmembrane Regions:

158-165
169-178
184-195
217-227
232-248
263-277
279-296
309-325
328-337
353-369
371-381
385-400
403-417
434-443
449-458
473-490
494-509
517-529
535-550
558-572
585-596
602-614

Protein Sequence:

>Vitamin B12 transporter BtuB
MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLAPTTVVTRQDIDRWQST
SVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDGVRLNLAGGSGSADLSQFP
IALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEISAGWGSNSYQNYDVSTQQQL
GDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFLSKTLYGALEHNFTDAWSGFVRG
YGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRYNGELIKSQLITSYSHSKDYNYDPH
YGRYDSSATLDEMKQYTVQWANNIIIGHGNVGAGVDWQKQSTAPGTAYVKDGYDQRNTGI
YLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQ
LYGFYGNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLIDYDDHTLKYYNEGKAR
IKGVEATANFDTGPLTHTVSYDYVDARNAITDTPLLRRAKQQVKYQLDWQLYDFDWGITY
QYLGTRYDKDYSSYPYQTVKMGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQ
TAGREYTLSGSYTF

References

External Links:

Resource	Link
Uniprot ID:	Q8X714
Uniprot Name:	BTUB_ECO57
GenBank Gene ID:	AE005174
Genebank Protein ID:	12518893
PDB ID:	1UJW
EchoBASE ID:	EB0124
CCDB:	BTUB_ECO57

General Reference:

Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K., Ishii, K., Yokoyama, K., Han, C. G., Ohtsubo, E., Nakayama, K., Murata, T., Tanaka, M., Tobe, T., Iida, T., Takami, H., Honda, T., Sasakawa, C., Ogasawara, N., Yasunaga, T., Kuhara, S., Shiba, T., Hattori, M., Shinagawa, H. (2001). "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." DNA Res 8:11-22. Pubmed: 11258796
Perna, N. T., Plunkett, G. 3rd, Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., Posfai, G., Hackett, J., Klink, S., Boutin, A., Shao, Y., Miller, L., Grotbeck, E. J., Davis, N. W., Lim, A., Dimalanta, E. T., Potamousis, K. D., Apodaca, J., Anantharaman, T. S., Lin, J., Yen, G., Schwartz, D. C., Welch, R. A., Blattner, F. R. (2001). "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Nature 409:529-533. Pubmed: 11206551

Vitamin B12 transporter BtuB (Q8X714)