DNA polymerase IV (Q47155)

Identification
Name:DNA polymerase IV
Synonyms:
  • Pol IV
Gene Name:dinB
Enzyme Class:
Biological Properties
General Function:Involved in damaged DNA binding
Specific Function:Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity. Overexpression of polIV results in increased frameshift mutagenesis. It is required for stationary-phase adaptive mutation, which provides the bacterium with flexibility in dealing with environmental stress, enhancing long-term survival and evolutionary fitness. May be involved in translesional synthesis, in conjunction with the beta clamp from polIII
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
damaged DNA binding
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA repair
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b0231
Gene OrientationClockwise
Centisome Percentage:5.41
Left Sequence End250898
Right Sequence End251953
Gene Sequence:
>1010 bp
TGTGGATATGGACTGCTTTTTCGCCGCAGTGGAGATGCGCGACAATCCCGCCCTGCGCGA
TATCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTA
TCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATG
CCCGCATCTCACCTTGCTTCCGGGGCGCTTTGACGCCTACAAAGAAGCCTCAAATCATAT
CCGCGAAATCTTCTCGCGCTACACCTCGCGTATTGAACCGTTGTCACTGGATGAGGCTTA
TCTCGACGTCACCGATAGCGTCCATTGCCACGGTTCTGCGACCCTCATCGCCCAGGAAAT
CCGCCAGACGATTTTCAACGAGCTGCAACTGACGGCGTCTGCGGGCGTGGCACCCGTAAA
GTTTCTCGCCAAAATCGCCTCCGACATGAATAAACCCAACGGCCAGTTTGTGATTACGCC
GGCAGAAGTTCCGGCATTTTTACAAACCTTACCACTGGCAAAAATCCCCGGCGTCGGCAA
AGTCTCGGCGGCAAAACTGGAAGCGATGGGGCTACGAACCTGCGGTGATGTACAAAAGTG
TGATCTGGTGATGCTGCTTAAACGCTTTGGCAAATTTGGCCGCATTTTGTGGGAGCGTAG
TCAGGGGATTGACGAGCGCGACGTTAACAGCGAACGGTTGCGAAAATCCGTCGGCGTGGA
ACGCACGATGGCGGAAGATATCCACCACTGGTCTGAATGTGAAGCGATTATCGAGCGGCT
GTATCCGGAACTTGAACGCCGTCTGGCAAAGGTAAAACCTGATTTACTGATTGCCCGCCA
GGGGGTGAAATTAAAGTTCGACGATTTTCAGCAAACCACTCAGGAGCACGTCTGGCCGCG
GCTGAATAAAGCTGACTTAATCGCCACCGCGCGTAAAACCTGGGATGAACGCCGCGGCGG
GCGCGGTGTGCGACTGGTGGGGCTGCATGTGACGTTGCTTGATCCGCAAA
Protein Properties
Pfam Domain Function:
Protein Residues:351
Protein Molecular Weight:39516
Protein Theoretical pI:10
PDB File:1UNN
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase IV
MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPARKFGVRSAMPTG
MALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLDEAYLDVTDSVHCHGSAT
LIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQFVITPAEVPAFLQTLPLAK
IPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFGRILWERSQGIDERDVNSERLR
KSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTTQ
EHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDPQMERQLVLGL
References
External Links:
ResourceLink
Uniprot ID:Q47155
Uniprot Name:DPO4_ECOLI
GenBank Gene ID:AF483081
Genebank Protein ID:19568865
PDB ID:1UNN
Ecogene ID:EG13141
Ecocyc:EG13141
ColiBase:b0231
Kegg Gene:b0231
EchoBASE ID:EB2935
CCDB:DPO4_ECOLI
BacMap:16128217
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bunting, K. A., Roe, S. M., Pearl, L. H. (2003). "Structural basis for recruitment of translesion DNA polymerase Pol IV/DinB to the beta-clamp." EMBO J 22:5883-5892. Pubmed: 14592985
  • Friedberg, E. C., Fischhaber, P. L., Kisker, C. (2001). "Error-prone DNA polymerases: novel structures and the benefits of infidelity." Cell 107:9-12. Pubmed: 11595180
  • Goodman, M. F. (2002). "Error-prone repair DNA polymerases in prokaryotes and eukaryotes." Annu Rev Biochem 71:17-50. Pubmed: 12045089
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kenyon, C. J., Walker, G. C. (1980). "DNA-damaging agents stimulate gene expression at specific loci in Escherichia coli." Proc Natl Acad Sci U S A 77:2819-2823. Pubmed: 6771759
  • Kim, S. R., Maenhaut-Michel, G., Yamada, M., Yamamoto, Y., Matsui, K., Sofuni, T., Nohmi, T., Ohmori, H. (1997). "Multiple pathways for SOS-induced mutagenesis in Escherichia coli: an overexpression of dinB/dinP results in strongly enhancing mutagenesis in the absence of any exogenous treatment to damage DNA." Proc Natl Acad Sci U S A 94:13792-13797. Pubmed: 9391106
  • Lenne-Samuel, N., Wagner, J., Etienne, H., Fuchs, R. P. (2002). "The processivity factor beta controls DNA polymerase IV traffic during spontaneous mutagenesis and translesion synthesis in vivo." EMBO Rep 3:45-49. Pubmed: 11751576
  • McKenzie, G. J., Lee, P. L., Lombardo, M. J., Hastings, P. J., Rosenberg, S. M. (2001). "SOS mutator DNA polymerase IV functions in adaptive mutation and not adaptive amplification." Mol Cell 7:571-579. Pubmed: 11463382
  • McKenzie, G. J., Rosenberg, S. M. (2001). "Adaptive mutations, mutator DNA polymerases and genetic change strategies of pathogens." Curr Opin Microbiol 4:586-594. Pubmed: 11587937
  • Napolitano, R., Janel-Bintz, R., Wagner, J., Fuchs, R. P. (2000). "All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis." EMBO J 19:6259-6265. Pubmed: 11080171
  • Ohmori, H., Hatada, E., Qiao, Y., Tsuji, M., Fukuda, R. (1995). "dinP, a new gene in Escherichia coli, whose product shows similarities to UmuC and its homologues." Mutat Res 347:1-7. Pubmed: 7596361
  • Wagner, J., Gruz, P., Kim, S. R., Yamada, M., Matsui, K., Fuchs, R. P., Nohmi, T. (1999). "The dinB gene encodes a novel E. coli DNA polymerase, DNA pol IV, involved in mutagenesis." Mol Cell 4:281-286. Pubmed: 10488344
  • Yeiser, B., Pepper, E. D., Goodman, M. F., Finkel, S. E. (2002). "SOS-induced DNA polymerases enhance long-term survival and evolutionary fitness." Proc Natl Acad Sci U S A 99:8737-8741. Pubmed: 12060704