Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (P76472)

Identification
Name:Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
Synonyms:Not Available
Gene Name:arnD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Specific Function:Catalyzes the deformylation of 4-deoxy-4-formamido-L- arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose- phosphoundecaprenol. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides (Probable)
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism ec00520
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Undecaprenyl phosphate-4-amino-4-formyl-L-arabinose → 1.0Formic acid + 1.0undecaprenyl phosphate-4-amino-4-deoxy-L-arabinose
ReactionCard
1.04-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate+1.0Thumb → 1.04-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate+1.0Thumb
1.04-deoxy-4-formamido-beta-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + 1.0Water → 1.04-amino-4-deoxy-alpha-L-arabinose di-trans,poly-cis-undecaprenyl phosphate + 1.0Formic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00142Formic acidMetaboCard
ECMDB21364undecaprenyl phosphate-4-amino-4-deoxy-L-arabinoseMetaboCard
ECMDB21365Undecaprenyl phosphate-4-amino-4-formyl-L-arabinoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b2256
Gene OrientationClockwise
Centisome Percentage:51.04
Left Sequence End2368040
Right Sequence End2368930
Gene Sequence:
>891 bp
ATGAGCGGCTTACCTCTTATTTCGCGCCGTCGACTGTTAACGGCGATGGCGCTTTCTCCG
TTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCG
CTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCG
GATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGAC
GTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATG
GTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGTATTGCGCCGGGTCGC
GGATTTAACTTCAGTGACGGCAAACAGCCGTTGGCGATGGCGCGTAAATCGCTGACGGAA
ATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGAC
TTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACG
ACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCAGGAAATT
CTTGATGAGTACGGCATCCCAAATGCCTGGCAAGGGGAAACCAACTTCTGGGGCAGTACC
GCCGTCAGTATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCAC
GACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTT
GTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCG
GCAATGCACTTTGTGCGCGTTCTGGATAACGCCATCGGAGGTAAAGCGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:296
Protein Molecular Weight:33112
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD
MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDNMGRHLWRLVKPQFLWK
MLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHEVGLHAWDHHAWQARSGNW
DRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIEAKEAFHLRYNSDCRGAMPFR
PLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLLNRILRDKGTPVYTIHAEVEGCA
YQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVVRGNIAGREGWLGCQQIAGSR
References
External Links:
ResourceLink
Uniprot ID:P76472
Uniprot Name:ARND_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674358
Ecogene ID:EG14092
Ecocyc:EG14092
ColiBase:b2256
Kegg Gene:b2256
EchoBASE ID:EB3845
CCDB:ARND_ECOLI
BacMap:16130191
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Breazeale, S. D., Ribeiro, A. A., McClerren, A. L., Raetz, C. R. (2005). "A formyltransferase required for polymyxin resistance in Escherichia coli and the modification of lipid A with 4-Amino-4-deoxy-L-arabinose. Identification and function oF UDP-4-deoxy-4-formamido-L-arabinose." J Biol Chem 280:14154-14167. Pubmed: 15695810
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yan, A., Guan, Z., Raetz, C. R. (2007). "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli." J Biol Chem 282:36077-36089. Pubmed: 17928292