N-acetylmuramoyl-L-alanine amidase AmiD (P75820)

Identification
Name:N-acetylmuramoyl-L-alanine amidase AmiD
Synonyms:Not Available
Gene Name:amiD
Enzyme Class:
Biological Properties
General Function:Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function:Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Cellular Location:Cell outer membrane; Lipid-anchor (Probable)
SMPDB Pathways:
  • 1,6-anhydro-<i>N</i>-acetylmuramic acid recycling PW002064
KEGG Pathways:Not Available
SMPDB Reactions:
1.0GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala+1.0Thumb → 1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate+1.0L-Ala-gamma-D-Glu-DAP-D-Ala
1.0GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala + 1.0Water → 1.0N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + 1.0L-Ala-gamma-D-Glu-DAP-D-Ala
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b0867
Gene OrientationClockwise
Centisome Percentage:19.49
Left Sequence End904136
Right Sequence End904966
Gene Sequence:
>831 bp
ATGTCGCAGGCTGCTCTGAACACGCGAAACAGCGAAGAAGAGGTTTCTTCTCGCCGCAAT
AATGGAACGCGTCTGGCGGGGATCCTTTTCCTGCTGACCGTTTTAACGACAGTGTTGGTG
AGCGGCTGGGTCGTGTTGGGCTGGATGGAAGATGCGCAACGCCTGCCGCTCTCAAAGCTG
GTGTTGACCGGTGAACGCCATTACACACGTAATGACGATATCCGGCAGTCGATCCTGGCA
TTGGGTGAGCCGGGTACCTTTATGACCCAGGATGTCAACATCATCCAGACGCAAATAGAA
CAACGCCTGCCGTGGATTAAGCAGGTGAGCGTCAGAAAGCAGTGGCCTGATGAATTGAAG
ATTCATCTGGTTGAATATGTGCCGATTGCGCGGTGGAATGATCAACATATGGTAGACGCG
GAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTG
TATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGGGCTATCGCGAAATGGGGCAGATG
CTGGCAAAGGACAGATTTACTCTGAAGGAAGCGGCGATGACCGCGCGGCGTTCCTGGCAG
TTGACGCTGAATAACGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTG
GCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGG
ATTAGCTACGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCG
CCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:276
Protein Molecular Weight:31072
Protein Theoretical pI:8
Signaling Regions:
  • 1-16
Transmembrane Regions:
  • None
Protein Sequence:
>N-acetylmuramoyl-L-alanine amidase AmiD
MRRFFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRIKVLVIHYTADDFDSSL
ATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGISAWRGATRLNDTSIGIEL
ENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKPENVVAHADIAPQRKDDPGPL
FPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASLLELLARYGYDVKPDMTPREQRR
VIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
References
External Links:
ResourceLink
Uniprot ID:P75820
Uniprot Name:AMID_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321974
Ecogene ID:EG13687
Ecocyc:EG13687
ColiBase:b0867
Kegg Gene:b0867
EchoBASE ID:EB3451
CCDB:AMID_ECOLI
BacMap:16128835
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232