ECMDB: Mannitol-specific cryptic phosphotransferase enzyme IIA component (P69824)

Identification

Name:

Mannitol-specific cryptic phosphotransferase enzyme IIA component

Synonyms:

EIIA-Mtl
EIII-Mtl
PTS system mannitol-specific EIIA component

Gene Name:

cmtB

Enzyme Class:

2.7.1.69

Biological Properties

General Function:

Involved in transporter activity

Specific Function:

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport

Cellular Location:

Cytoplasm (Probable)

SMPDB Pathways:

Galactose metabolism PW000821
Starch and sucrose metabolism PW000941

KEGG Pathways:

Amino sugar and nucleotide sugar metabolism ec00520
Ascorbate and aldarate metabolism ec00053
Fructose and mannose metabolism ec00051
Galactose metabolism ec00052
Glycolysis / Gluconeogenesis ec00010
Metabolic pathways eco01100
Microbial metabolism in diverse environments ec01120
Pentose phosphate pathway ec00030
Starch and sucrose metabolism ec00500

KEGG Reactions:

1.0Protein N(pi)-phospho-L-histidine

1.0

↔

1.0Protein histidine

1.0

1.0Protein N(pi)-phospho-L-histidine + 1.0Mannitol ↔ 1.0Protein histidine + 1.0Sorbitol-6-phosphate
ReactionCard

1.0Protein N(pi)-phospho-L-histidine

1.0Sugar

↔

1.0Protein histidine

1.0

1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard

Complex Reactions:

1.0Protein EIIA N(pi)-phospho-L-histidine

1.0protein EIIB

→

1.0protein EIIA

1.0protein EIIB N(pi)-phospho-L-histidine/cysteine

1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00765	Mannitol	MetaboCard
ECMDB21434	Mannitol 1-phosphate	MetaboCard
ECMDB01400	Sorbitol-6-phosphate	MetaboCard

GO Classification:

Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport

Gene Properties

Blattner:

b2934

Gene Orientation

Counterclockwise

Centisome Percentage:

66.32

Left Sequence End

3076909

Right Sequence End

3077352

Gene Sequence:

>444 bp
ATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGAT
GCCGCGCGCCTGCTGGGTGAAATGCGTCGCGGATGTCAGCAGGCGGAAGAACAGCTCAAA
ATGCTGATTGATTATCAGAATGAATATCGCAATAACCTCAACAGCGATATGAGTGCCGGG
ATAACCAGCAACCGCTGGATCAACTATCAGCAGTTTATCCAGACGCTGGAAAAAGCCATT
ACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGT
TGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACG
GCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGGATGAGTTCGCCCAGCGC
GCCGCCATGAGGAAACCTGAATGA

Protein Properties

Pfam Domain Function:

PTS_EIIA_2 (PF00359 )

Protein Residues:

147

Protein Molecular Weight:

16046

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Mannitol-specific cryptic phosphotransferase enzyme IIA component
MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILA
PGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
LLCEEEILEQLLTASSEKQLADIISRG

References

External Links:

Resource	Link
Uniprot ID:	P69824
Uniprot Name:	PTMA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1736608
Ecogene ID:	EG11791
Ecocyc:	EG11791
ColiBase:	b2934
Kegg Gene:	b2934
EchoBASE ID:	EB1739
CCDB:	PTMA_ECOLI
BacMap:	16130835

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Sprenger, G. A. (1993). "Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria." Biochim Biophys Acta 1158:103-106. Pubmed: 8353127