N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component (P69791)

Identification
Name:N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component
Synonyms:
  • EIIA-Chb
  • EIII-Chb
  • PTS system N,N'-diacetylchitobiose-specific EIIA component
Gene Name:chbA
Enzyme Class:
Biological Properties
General Function:Involved in sugar:hydrogen symporter activity
Specific Function:The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in N,N'-diacetylchitobiose transport
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Protein N(pi)-phospho-L-histidine+1.0Sugar ↔ 1.0Protein histidine+1.0Thumb
1.0Protein N(pi)-phospho-L-histidine + 1.0Sugar ↔ 1.0Protein histidine + 1.0Sugar phosphate
ReactionCard
Complex Reactions:
1.0Protein EIIA N(pi)-phospho-L-histidine+1.0protein EIIB → 1.0protein EIIA+1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
1.0Protein EIIA N(pi)-phospho-L-histidine + 1.0protein EIIB → 1.0protein EIIA + 1.0protein EIIB N(pi)-phospho-L-histidine/cysteine
ReactionCard
Metabolites:
ECMDB IDNameView
GO Classification:
Component
cell part
membrane
Function
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
sugar:hydrogen symporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
phosphoenolpyruvate-dependent sugar phosphotransferase system
transport
Gene Properties
Blattner:b1736
Gene OrientationCounterclockwise
Centisome Percentage:39.17
Left Sequence End1817479
Right Sequence End1817829
Gene Sequence:
>351 bp
ATGATGGATCTCGATAACATTCCCGATACGCAAACGGAAGCTGAAGAGCTGGAAGAAGTG
GTGATGGGGCTGATCATCAACTCCGGACAAGCGCGCAGCCTGGCGTATGCGGCACTGAAA
CAGGCGAAGCAGGGCGATTTTGCCGCAGCAAAAGCCATGATGGATCAGTCACGGATGGCA
TTGAATGAAGCGCATCTGGTACAGACGAAACTGATTGAAGGCGATGCGGGCGAAGGTAAG
ATGAAAGTGAGTCTGGTGCTGGTCCACGCTCAGGATCATTTAATGACGTCCATGCTTGCG
CGTGAACTGATTACTGAATTAATTGAGCTTCATGAAAAACTGAAGGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:116
Protein Molecular Weight:12748
Protein Theoretical pI:5
PDB File:1WCR
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component
MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQGDFAAAKAMMDQSRMA
LNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARELITELIELHEKLKA
References
External Links:
ResourceLink
Uniprot ID:P69791
Uniprot Name:PTQA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742833
PDB ID:1WCR
Ecogene ID:EG10142
Ecocyc:EG10142
ColiBase:b1736
Kegg Gene:b1736
EchoBASE ID:EB0140
CCDB:PTQA_ECOLI
BacMap:16129690
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hall, B. G., Sharp, P. M. (1992). "Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates." Mol Biol Evol 9:654-665. Pubmed: 1630305
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Keyhani, N. O., Boudker, O., Roseman, S. (2000). "Isolation and characterization of IIAChb, a soluble protein of the enzyme II complex required for the transport/phosphorylation of N, N'-diacetylchitobiose in Escherichia coli." J Biol Chem 275:33091-33101. Pubmed: 10913118
  • Keyhani, N. O., Roseman, S. (1997). "Wild-type Escherichia coli grows on the chitin disaccharide, N,N'-diacetylchitobiose, by expressing the cel operon." Proc Natl Acad Sci U S A 94:14367-14371. Pubmed: 9405618
  • Keyhani, N. O., Wang, L. X., Lee, Y. C., Roseman, S. (2000). "The chitin disaccharide, N,N'-diacetylchitobiose, is catabolized by Escherichia coli and is transported/phosphorylated by the phosphoenolpyruvate:glycose phosphotransferase system." J Biol Chem 275:33084-33090. Pubmed: 10913117
  • Keyhani, N., Rodgers, M. E., Demeler, B., Hansen, J. C., Roseman, S. (2000). "Analytical sedimentation of the IIAChb and IIBChb proteins of the Escherichia coli N,N'-diacetylchitobiose phosphotransferase system. Demonstration of a model phosphotransfer transition state complex." J Biol Chem 275:33110-33115. Pubmed: 10913122
  • Parker, L. L., Hall, B. G. (1990). "Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12." Genetics 124:455-471. Pubmed: 2179047