Phosphoheptose isomerase (P63224)

Identification
Name:Phosphoheptose isomerase
Synonyms:
  • Sedoheptulose 7-phosphate isomerase
Gene Name:gmhA
Enzyme Class:
Biological Properties
General Function:Involved in D-sedoheptulose 7-phosphate isomerase activity
Specific Function:Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Sedoheptulose 7-phosphate ↔ 1.0D-glycero-beta-D-manno-Heptose 7-phosphate
1.0Sedoheptulose 7-phosphate ↔ 1.0D-glycero-beta-D-manno-Heptose 7-phosphate
ReactionCard
1.0Sedoheptulose 7-phosphate+1.0Thumb ↔ 1.0Thumb
1.0Sedoheptulose 7-phosphate + 1.0D-Sedoheptulose 7-phosphate ↔ 1.0D-Glycero-D-manno-heptose 7-phosphate
ReactionCard
1.0Sedoheptulose 7-phosphate ↔ 1.0D-glycero-alpha-D-manno-Heptose 7-phosphate
1.0Sedoheptulose 7-phosphate ↔ 1.0D-glycero-alpha-D-manno-Heptose 7-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb → 1.0Thumb
1.0D-Sedoheptulose 7-phosphate → 1.0D-Glycero-D-manno-heptose 7-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23038D-glycero-beta-D-manno-heptose 7-phosphateMetaboCard
ECMDB21201D-Glycero-D-manno-heptose 7-phosphateMetaboCard
ECMDB01068D-Sedoheptulose 7-phosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
carbohydrate binding
catalytic activity
D-sedoheptulose 7-phosphate isomerase activity
intramolecular transferase activity
intramolecular transferase activity, phosphotransferases
isomerase activity
sugar binding
Process
carbohydrate metabolic process
lipopolysaccharide core region biosynthetic process
metabolic process
oligosaccharide biosynthetic process
oligosaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b0222
Gene OrientationClockwise
Centisome Percentage:5.25
Left Sequence End243543
Right Sequence End244121
Gene Sequence:
>579 bp
ATGTACCAGGATCTTATTCGTAACGAACTGAACGAAGCGGCGGAAACGCTGGCTAACTTT
TTAAAAGATGACGCCAATATTCACGCCATTCAGCGCGCGGCGGTCCTGTTAGCAGACAGC
TTTAAAGCCGGTGGCAAAGTGCTTTCCTGCGGCAACGGCGGTTCCCATTGCGACGCTATG
CACTTTGCCGAAGAGTTGACCGGTCGCTACCGTGAAAACCGTCCGGGCTACCCGGCGATT
GCTATTTCTGACGTTAGTCATATTTCCTGCGTCGGTAATGATTTCGGTTTCAATGATATT
TTCTCCCGCTACGTTGAAGCGGTAGGTCGCGAAGGCGATGTACTGCTGGGGATCTCCACC
TCCGGTAACTCTGCAAACGTGATCAAAGCGATCGCAGCGGCGCGTGAGAAGGGAATGAAA
GTGATCACCCTGACCGGTAAAGACGGCGGCAAAATGGCTGGCACGGCGGATATCGAAATT
CGCGTACCGCACTTTGGTTATGCCGACCGCATTCAGGAGATTCACATTAAAGTGATCCAT
ATCCTGATCCAGTTGATTGAAAAAGAGATGGTTAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:192
Protein Molecular Weight:20815
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphoheptose isomerase
MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAGGKVLSCGNGGSHCDAM
HFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFSRYVEAVGREGDVLLGIST
SGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEIRVPHFGYADRIQEIHIKVIH
ILIQLIEKEMVK
References
External Links:
ResourceLink
Uniprot ID:P63224
Uniprot Name:GMHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902958
Ecogene ID:EG13146
Ecocyc:EG13146
ColiBase:b0222
Kegg Gene:b0222
EchoBASE ID:EB2940
CCDB:GMHA_ECOLI
BacMap:16128208
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brooke, J. S., Valvano, M. A. (1996). "Biosynthesis of inner core lipopolysaccharide in enteric bacteria identification and characterization of a conserved phosphoheptose isomerase." J Biol Chem 271:3608-3614. Pubmed: 8631969
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kneidinger, B., Marolda, C., Graninger, M., Zamyatina, A., McArthur, F., Kosma, P., Valvano, M. A., Messner, P. (2002). "Biosynthesis pathway of ADP-L-glycero-beta-D-manno-heptose in Escherichia coli." J Bacteriol 184:363-369. Pubmed: 11751812
  • Raetz, C. R., Whitfield, C. (2002). "Lipopolysaccharide endotoxins." Annu Rev Biochem 71:635-700. Pubmed: 12045108
  • Valvano, M. A., Messner, P., Kosma, P. (2002). "Novel pathways for biosynthesis of nucleotide-activated glycero-manno-heptose precursors of bacterial glycoproteins and cell surface polysaccharides." Microbiology 148:1979-1989. Pubmed: 12101286