2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (P62617)

Identification
Name:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
Synonyms:
  • MECDP-synthase
  • MECPS
Gene Name:ispF
Enzyme Class:
Biological Properties
General Function:Involved in 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
Specific Function:Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP. Also converts 4-diphosphocytidyl-2-C-methyl-D- erythritol into 2-C-methyl-D-erythritol 3,4-cyclophosphate and CMP
Cellular Location:Not Available
SMPDB Pathways:
  • Operon: cytidyl pyrophosphate biosynthesis of isoprenoids PW000988
  • Secondary metabolites: isoprenoid biosynthesis (nonmevalonate pathway) PW000975
  • Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol ↔ 1.02-C-Methyl-D-erythritol-2,4-cyclodiphosphate + 1.0Cytidine monophosphate
ReactionCard
SMPDB Reactions:
1.02-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol+1.0Thumb → 1.0Cytidine monophosphate+1.0Thumb+1.0Thumb
1.02-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol → 1.0Cytidine monophosphate + 1.02-C-Methyl-D-erythritol-2,4-cyclodiphosphate + 1.0Cytidine monophosphate
ReactionCard
1.0Thumb+1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol → 1.0Thumb+1.0Cytidine monophosphate+1.0Thumb
1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol → 1.02-C-Methyl-D-erythritol 2,4-cyclodiphosphate + 1.0Cytidine monophosphate + 1.0Cytidine monophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol ↔ 1.02-C-Methyl-D-erythritol-2,4-cyclodiphosphate + 1.0Cytidine monophosphate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol → 1.02-C-Methyl-D-erythritol 2,4-cyclodiphosphate + 1.0Cytidine monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB231122-C-Methyl-D-erythritol 2,4-cyclodiphosphateMetaboCard
ECMDB041032-C-Methyl-D-erythritol-2,4-cyclodiphosphateMetaboCard
ECMDB200492-Phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritolMetaboCard
ECMDB00095Cytidine monophosphateMetaboCard
GO Classification:
Function
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity
catalytic activity
lyase activity
phosphorus-oxygen lyase activity
Process
cellular lipid metabolic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
primary metabolic process
terpenoid biosynthetic process
terpenoid metabolic process
Gene Properties
Blattner:b2746
Gene OrientationCounterclockwise
Centisome Percentage:61.84
Left Sequence End2869323
Right Sequence End2869802
Gene Sequence:
>480 bp
ATGCGAATTGGACACGGTTTTGACGTACATGCCTTTGGCGGTGAAGGCCCAATTATCATT
GGTGGCGTACGCATTCCTTACGAAAAAGGATTGCTGGCGCATTCTGATGGCGACGTGGCG
CTCCATGCGTTGACCGATGCATTGCTTGGCGCGGCGGCGCTGGGGGATATCGGCAAGCTG
TTCCCGGATACCGATCCGGCATTTAAAGGTGCCGATAGCCGCGAGCTGCTACGCGAAGCC
TGGCGTCGTATTCAGGCGAAGGGTTATACCCTTGGCAACGTCGATGTCACTATCATCGCT
CAGGCACCGAAGATGTTGCCGCACATTCCACAAATGCGCGTGTTTATTGCCGAAGATCTC
GGCTGCCATATGGATGATGTTAACGTGAAAGCCACTACTACGGAAAAACTGGGATTTACC
GGACGTGGGGAAGGGATTGCCTGTGAAGCGGTGGCGCTACTCATTAAGGCAACAAAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:159
Protein Molecular Weight:16897
Protein Theoretical pI:7
PDB File:1U43
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHALTDALLGAAALGDIGKL
FPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQAPKMLPHIPQMRVFIAEDL
GCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK
References
External Links:
ResourceLink
Uniprot ID:P62617
Uniprot Name:ISPF_ECOLI
GenBank Gene ID:AB038256
Genebank Protein ID:7678764
PDB ID:1U43
Ecogene ID:EG11816
Ecocyc:EG11816
ColiBase:b2746
Kegg Gene:b2746
EchoBASE ID:EB1763
CCDB:ISPF_ECOLI
BacMap:16130653
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Herz, S., Wungsintaweekul, J., Schuhr, C. A., Hecht, S., Luttgen, H., Sagner, S., Fellermeier, M., Eisenreich, W., Zenk, M. H., Bacher, A., Rohdich, F. (2000). "Biosynthesis of terpenoids: YgbB protein converts 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate to 2C-methyl-D-erythritol 2,4-cyclodiphosphate." Proc Natl Acad Sci U S A 97:2486-2490. Pubmed: 10694574
  • Kemp, L. E., Bond, C. S., Hunter, W. N. (2002). "Structure of 2C-methyl-D-erythritol 2,4- cyclodiphosphate synthase: an essential enzyme for isoprenoid biosynthesis and target for antimicrobial drug development." Proc Natl Acad Sci U S A 99:6591-6596. Pubmed: 11997478
  • Li, C., Ichikawa, J. K., Ravetto, J. J., Kuo, H. C., Fu, J. C., Clarke, S. (1994). "A new gene involved in stationary-phase survival located at 59 minutes on the Escherichia coli chromosome." J Bacteriol 176:6015-6022. Pubmed: 7928962
  • Richard, S. B., Ferrer, J. L., Bowman, M. E., Lillo, A. M., Tetzlaff, C. N., Cane, D. E., Noel, J. P. (2002). "Structure and mechanism of 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase. An enzyme in the mevalonate-independent isoprenoid biosynthetic pathway." J Biol Chem 277:8667-8672. Pubmed: 11786530
  • Steinbacher, S., Kaiser, J., Wungsintaweekul, J., Hecht, S., Eisenreich, W., Gerhardt, S., Bacher, A., Rohdich, F. (2002). "Structure of 2C-methyl-d-erythritol-2,4-cyclodiphosphate synthase involved in mevalonate-independent biosynthesis of isoprenoids." J Mol Biol 316:79-88. Pubmed: 11829504