Undecaprenyl pyrophosphate synthase (P60472)

Identification
Name:Undecaprenyl pyrophosphate synthase
Synonyms:
  • UPP synthase
  • Di-trans,poly-cis-decaprenylcistransferase
  • Undecaprenyl diphosphate synthase
  • UDS
Gene Name:uppS
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring alkyl or aryl (other than methyl) groups
Specific Function:Generates undecaprenyl pyrophosphate (UPP) from isopentenyl pyrophosphate (IPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958
KEGG Pathways:
  • Terpenoid backbone biosynthesis ec00900
KEGG Reactions:
1.0Thumb+8.0Thumb ↔ 1.0di-trans,poly-cis-Undecaprenyl diphosphate+8.0Thumb+1.0Thumb
1.0Farnesyl pyrophosphate + 8.0Isopentenyl pyrophosphate ↔ 1.0di-trans,poly-cis-Undecaprenyl diphosphate + 8.0Pyrophosphate + 1.0Undecaprenyl diphosphate
ReactionCard
SMPDB Reactions:
1.0Farnesyl pyrophosphate+8.0Isopentenyl pyrophosphate+1.0Thumb+8.0Thumb → 8.0Pyrophosphate+1.0di-trans,octa-cis-undecaprenyl diphosphate
1.0Farnesyl pyrophosphate + 8.0Isopentenyl pyrophosphate + 1.0Farnesyl pyrophosphate + 8.0Isopentenyl pyrophosphate → 8.0Pyrophosphate + 1.0di-trans,octa-cis-undecaprenyl diphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+8.0Thumb → 8.0Thumb+1.0Thumb
1.0Farnesyl pyrophosphate + 8.0Isopentenyl pyrophosphate → 8.0Pyrophosphate + 1.0Undecaprenyl diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00961Farnesyl pyrophosphateMetaboCard
ECMDB01347Isopentenyl pyrophosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01469Undecaprenyl diphosphateMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Gene Properties
Blattner:b0174
Gene OrientationClockwise
Centisome Percentage:4.20
Left Sequence End194903
Right Sequence End195664
Gene Sequence:
>762 bp
GTGATGTTGTCTGCTACTCAACCACTTAGCGAAAAATTGCCAGCGCATGGCTGCCGTCAT
GTTGCGATCATTATGGACGGCAATGGCCGCTGGGCAAAAAAGCAAGGGAAGATTCGTGCC
TTTGGGCATAAAGCCGGGGCAAAATCCGTCCGCCGGGCTGTCTCTTTTGCGGCCAACAAC
GGTATTGAGGCGTTAACGCTGTATGCCTTTAGTAGTGAAAACTGGAACCGACCAGCGCAG
GAAGTCAGTGCGTTAATGGAACTGTTTGTGTGGGCGCTCGATAGCGAAGTAAAAAGTCTG
CACCGACATAACGTGCGTCTGCGTATTATTGGCGATACCAGTCGCTTTAACTCGCGTTTG
CAAGAACGTATTCGTAAATCTGAAGCGCTAACAGCCGGGAATACCGGTCTGACGCTGAAT
ATTGCGGCGAACTACGGTGGACGTTGGGATATAGTCCAGGGAGTCAGGCAACTGGCTGAA
AAGGTGCAGCAAGGAAACCTGCAACCAGATCAGATAGATGAAGAGATGCTAAACCAGCAT
GTCTGTATGCATGAACTGGCCCCTGTAGATTTAGTAATTAGGACTGGGGGGGAGCATCGC
ATTAGTAACTTTTTGCTTTGGCAAATTGCCTATGCCGAACTTTACTTTACAGATGTTCTC
TGGCCCGATTTCGATGAACAAGACTTTGAAGGGGCGTTAAATGCCTTTGCTAATCGAGAG
CGTCGTTTCGGCGGCACCGAGCCCGGTGATGAAACAGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:253
Protein Molecular Weight:28444
Protein Theoretical pI:7
PDB File:1X07
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Undecaprenyl pyrophosphate synthase
MMLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKAGAKSVRRAVSFAANN
GIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRHNVRLRIIGDTSRFNSRL
QERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEKVQQGNLQPDQIDEEMLNQH
VCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDVLWPDFDEQDFEGALNAFANRE
RRFGGTEPGDETA
References
External Links:
ResourceLink
Uniprot ID:P60472
Uniprot Name:UPPS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674367
PDB ID:1X07
Ecogene ID:EG13329
Ecocyc:EG13329
ColiBase:b0174
Kegg Gene:b0174
EchoBASE ID:EB3113
CCDB:UPPS_ECOLI
BacMap:16128167
General Reference:
  • Apfel, C. M., Takacs, B., Fountoulakis, M., Stieger, M., Keck, W. (1999). "Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: cloning, expression, and characterization of the essential uppS gene." J Bacteriol 181:483-492. Pubmed: 9882662
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chang, S. Y., Ko, T. P., Liang, P. H., Wang, A. H. (2003). "Catalytic mechanism revealed by the crystal structure of undecaprenyl pyrophosphate synthase in complex with sulfate, magnesium, and triton." J Biol Chem 278:29298-29307. Pubmed: 12756244
  • Chen, Y. H., Chen, A. P., Chen, C. T., Wang, A. H., Liang, P. H. (2002). "Probing the conformational change of Escherichia coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants." J Biol Chem 277:7369-7376. Pubmed: 11744728
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kato, J., Fujisaki, S., Nakajima, K., Nishimura, Y., Sato, M., Nakano, A. (1999). "The Escherichia coli homologue of yeast RER2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis." J Bacteriol 181:2733-2738. Pubmed: 10217761
  • Ko, T. P., Chen, Y. K., Robinson, H., Tsai, P. C., Gao, Y. G., Chen, A. P., Wang, A. H., Liang, P. H. (2001). "Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis." J Biol Chem 276:47474-47482. Pubmed: 11581264
  • Pan, J. J., Yang, L. W., Liang, P. H. (2000). "Effect of site-directed mutagenesis of the conserved aspartate and glutamate on E. coli undecaprenyl pyrophosphate synthase catalysis." Biochemistry 39:13856-13861. Pubmed: 11076526